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The map label for this gene is tktB

Identifier: 218700921

GI number: 218700921

Start: 2696407

End: 2698410

Strand: Direct

Name: tktB

Synonym: ECIAI39_2602

Alternate gene names: 218700921

Gene position: 2696407-2698410 (Clockwise)

Preceding gene: 218700920

Following gene: 218700925

Centisome position: 52.54

GC content: 53.89

Gene sequence:

>2004_bases
ATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGG
CGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAATCCTACCGACCCAACCTGGT
ATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTGCATCTGACCGGTTACGAC
CTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAAATCGGCTATACGCCTGG
TGTTGAAACCACCACCGGTCCACTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCAATAGCAGAGCGTACACTGGCGG
CGCAGTTTAACCAGCCAGATCATGAAATTGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGT
ATTTCCCACGAAGTCTGTTCGCTGGCGGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTC
CATCGACGGTGAAACCGAAGGCTGGTTTACCGACGATACAGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCACG
AGATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATT
ATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGA
AGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGG
ATGCCCGCGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTCATCCTCAACTG
GCAGAAGAGTTTACCCGTCGGATGAGCGGTGGTTTACCGAAGGACTGGGAGAAAACGACTCAGAAATATATCAATGAGTT
GCAGGCGAATCCGGCGAAAATCGCTACCCGTAAGGCTTCGCAAAATACGCTTAACGCTTACGGGCCGATGCTGCCTGAGT
TGCTCGGCGGTTCGGCGGATCTGGCTCCCAGCAACCTGACCATCTGGAAAGGTTCTGTTTCGCTGAAGGAAGATCCGGCG
GGCAACTACATTCACTACGGAGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATTGCGCACCACGGCGGCTTTGT
GCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGC
AGATTATGGTTTATACCCACGACTCAATTGGCTTAGGCGAAGATGGTCCTACGCACCAGGCTGTTGAGCAACTGGCCAGT
CTGCGCTTAACGCCAAATTTCAGCACCTGGCGACCGTGCGATCAGGTGGAAGCAGCGGTGGGCTGGAAGCTGGCGGTTGA
GCGCCACAACGGACCGACGGCACTGATCCTCTCCAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAG
AGATTGCTCGTGGCGGTTATGTACTGAAAGACAGCGGCGGTAAGCCAGATATTATTTTGATTGCCACCGGTTCAGAGATG
GAAATCACCCTGCAAGCGGCAGAGAAATTAGCGGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATAT
TTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCAAATGTTTCTGCCCGCGTGGCAGTAGAAGCGGGTA
TTGCCGATTACTGGTACAAATATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACAGGTTATGGGGAATCTGCTCCGGCG
GATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTACTGGGAGTGAAAGGTGC
CTGA

Upstream 100 bases:

>100_bases
GTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATA
AACCAGCCACGGAGTGTTAT

Downstream 100 bases:

>100_bases
TGGTGATCGCCGGATGCTGATTGTCGGATGTGACGCTGGCGCGTCTTATCCGGTCTACATGTCCCCGCCATTTTGTTTAA
CGGGTGATCCACAACGTGGG

Product: transketolase

Products: NA

Alternate protein names: TK 2 [H]

Number of amino acids: Translated: 667; Mature: 666

Protein sequence:

>667_residues
MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYD
LPLEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLI
ICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQL
AEEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPA
GNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLAS
LRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEM
EITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVSARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPA
DKLFPFFGFTAENIVAKAHKVLGVKGA

Sequences:

>Translated_667_residues
MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYD
LPLEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLI
ICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQL
AEEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPA
GNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLAS
LRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEM
EITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVSARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPA
DKLFPFFGFTAENIVAKAHKVLGVKGA
>Mature_666_residues
SRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDL
PLEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEGI
SHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLII
CRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLA
EEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPAG
NYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLASL
RLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEME
ITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVSARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPAD
KLFPFFGFTAENIVAKAHKVLGVKGA

Specific function: Unknown

COG id: COG0021

COG function: function code G; Transketolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI205277463, Length=579, Percent_Identity=29.1882556131261, Blast_Score=173, Evalue=6e-43,
Organism=Homo sapiens, GI4507521, Length=579, Percent_Identity=29.1882556131261, Blast_Score=173, Evalue=6e-43,
Organism=Homo sapiens, GI133778974, Length=678, Percent_Identity=26.8436578171091, Blast_Score=172, Evalue=8e-43,
Organism=Escherichia coli, GI1788808, Length=667, Percent_Identity=99.8500749625187, Blast_Score=1380, Evalue=0.0,
Organism=Escherichia coli, GI48994911, Length=661, Percent_Identity=75.4916792738275, Blast_Score=1078, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17539652, Length=671, Percent_Identity=25.6333830104322, Blast_Score=160, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325331, Length=666, Percent_Identity=48.3483483483483, Blast_Score=596, Evalue=1e-171,
Organism=Saccharomyces cerevisiae, GI6319593, Length=668, Percent_Identity=45.059880239521, Blast_Score=548, Evalue=1e-157,
Organism=Drosophila melanogaster, GI45551847, Length=597, Percent_Identity=27.8056951423786, Blast_Score=165, Evalue=8e-41,
Organism=Drosophila melanogaster, GI45550715, Length=597, Percent_Identity=27.8056951423786, Blast_Score=165, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24645119, Length=557, Percent_Identity=27.4685816876122, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24666278, Length=294, Percent_Identity=32.6530612244898, Blast_Score=129, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005478
- InterPro:   IPR020826
- InterPro:   IPR005476
- InterPro:   IPR005474 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C; PF00456 Transketolase_N [H]

EC number: =2.2.1.1 [H]

Molecular weight: Translated: 73059; Mature: 72928

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00801 TRANSKETOLASE_1 ; PS00802 TRANSKETOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
LSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLA
EECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
NAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEGISHEVCSLAGTLGLGKLIGF
HHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHE
YDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLI
ECCCCEEECCCCCCCCCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEE
ICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKEIYHAWDAREKGE
EEEHHHCCCCCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHH
KAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKAS
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCC
QNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPAGNYIHYGVREFGMTAIANGI
CCHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
AHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLAS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHH
LRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGY
EECCCCCCCCCCHHHHHHHHCEEEEEEECCCCEEEEEECHHHHHHHCCHHHHHHHHHCCE
VLKDSGGKPDIILIATGSEMEITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVL
EEECCCCCCCEEEEEECCCEEEEHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHC
PSNVSARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHK
CCCCCEEEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCHHHCCCHHHHHHHHHH
VLGVKGA
HHCCCCC
>Mature Secondary Structure 
SRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFI
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
LSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLA
EECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
NAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEGISHEVCSLAGTLGLGKLIGF
HHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHE
YDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLI
ECCCCEEECCCCCCCCCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEE
ICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKEIYHAWDAREKGE
EEEHHHCCCCCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHH
KAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKAS
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCC
QNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPAGNYIHYGVREFGMTAIANGI
CCHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
AHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLAS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHH
LRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGY
EECCCCCCCCCCHHHHHHHHCEEEEEEECCCCEEEEEECHHHHHHHCCHHHHHHHHHCCE
VLKDSGGKPDIILIATGSEMEITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVL
EEECCCCCCCEEEEEECCCEEEEHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHC
PSNVSARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHK
CCCCCEEEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCHHHCCCHHHHHHHHHH
VLGVKGA
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8396116; 9205837; 9278503 [H]