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The map label for this gene is ddg

Identifier: 218700847

GI number: 218700847

Start: 2617442

End: 2618362

Strand: Direct

Name: ddg

Synonym: ECIAI39_2522

Alternate gene names: 218700847

Gene position: 2617442-2618362 (Clockwise)

Preceding gene: 218700844

Following gene: 218700849

Centisome position: 51.0

GC content: 51.25

Gene sequence:

>921_bases
ATGTTTCCAAAATGCAAATTTTCCCGCGAGTTTCTGCATCCTCGCTACTGGCTCACGTGGTTTGGGCTTGGTGTACTCTG
GCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATCGGCACAATGGCGCGACCATTCCTGAAAC
GTCGTGAATCTATCGCCCGTAAAAACCTGGAACTTTGCTTCCCGCAGCATTCTGCGGAAGAACGCGAGAAGATGATTGCC
GAAAACTTTCGTTCACTCGGCATGGCGCTGGTAGAAACCGGCATGGCATGGTTCTGGCCCGACAGTCGCGTACGTAAATG
GTTTGATGTGGAAGGGCTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTA
TGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTTTGTCAGCCAATGATGGCCACCTATCGTCCACATAATAATCAGCTG
ATGGAATGGGTGCAGACCCGTGGTCGCATGCGCTCTAACAAAGCGATGATCGGCAGAAATAATCTGCGCGGCATTGTCGG
TGCACTGAAGAAAGGTGAAGCGGTATGGTTTGCTCCCGATCAGGATTATGGTCGTAAAGGCAGCTCCTTCGCGCCGTTCT
TTGCGGTGGAAAATGTTGCCACAACCAATGGTACGTATGTCCTTTCACGTCTCTCTGGCGCAGCCATGTTAACCGTAACG
ATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTACATCACCCCAGAGATGGAAGGCTACCCGGCAGATGAAAATCA
AGCCGCTGCCTATATGAACAAGATTATCGAAAAAGAGATCATGCGCGCACCGGAGCAGTACCTATGGATCCACCGTCGCT
TTAAAACGCGCCCGGTGGGAGAATCGTCGCTATACATTTAA

Upstream 100 bases:

>100_bases
CAGGAACCATTGTCGTACATGATGGCCTAATCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATT
CGTATCTACAGAAGGTAACT

Downstream 100 bases:

>100_bases
CAAACGGCTCTTACTCTTTCCCTCCTCAAATCAGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTT
CCTTCGCTGAATCTCTAATT

Product: lipid A biosynthesis palmitoleoyl acyltransferase

Products: KDO2-(palmitoleoyl)-lipid IVA [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGTMARPFLKRRESIARKNLELCFPQHSAEEREKMIA
ENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVENVATTNGTYVLSRLSGAAMLTVT
MVRKADYSGYRLYITPEMEGYPADENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRPVGESSLYI

Sequences:

>Translated_306_residues
MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGTMARPFLKRRESIARKNLELCFPQHSAEEREKMIA
ENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVENVATTNGTYVLSRLSGAAMLTVT
MVRKADYSGYRLYITPEMEGYPADENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRPVGESSLYI
>Mature_306_residues
MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGTMARPFLKRRESIARKNLELCFPQHSAEEREKMIA
ENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVENVATTNGTYVLSRLSGAAMLTVT
MVRKADYSGYRLYITPEMEGYPADENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRPVGESSLYI

Specific function: Unknown

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI87082094, Length=306, Percent_Identity=98.6928104575163, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1787292, Length=301, Percent_Identity=54.8172757475083, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1788161, Length=307, Percent_Identity=26.3843648208469, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: NA

Molecular weight: Translated: 35510; Mature: 35510

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGTMARPFLKRRESIAR
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQ
CCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCCHHHHHHHH
MQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQLMEWVQTRGRMRSNKAMIGRN
HHCCCEEEEEEEEEEHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCC
NLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVENVATTNGTYVLSRLSGAAMLTVT
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHEECCCCCCCCCEEEEHHCCCHHHHHH
MVRKADYSGYRLYITPEMEGYPADENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRPVG
HHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
ESSLYI
CCCCCC
>Mature Secondary Structure
MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGTMARPFLKRRESIAR
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQ
CCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCCHHHHHHHH
MQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQLMEWVQTRGRMRSNKAMIGRN
HHCCCEEEEEEEEEEHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCC
NLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVENVATTNGTYVLSRLSGAAMLTVT
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHEECCCCCCCCCEEEEHHCCCHHHHHH
MVRKADYSGYRLYITPEMEGYPADENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRPVG
HHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
ESSLYI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; palmitoleoyl-ACP [C]

Specific reaction: KDO2-lipid IVA + palmitoleoyl-ACP = KDO2-(palmitoleoyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]