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The map label for this gene is yfcG

Identifier: 218700776

GI number: 218700776

Start: 2537906

End: 2538553

Strand: Direct

Name: yfcG

Synonym: ECIAI39_2449

Alternate gene names: 218700776

Gene position: 2537906-2538553 (Clockwise)

Preceding gene: 218700772

Following gene: 218700777

Centisome position: 49.45

GC content: 51.85

Gene sequence:

>648_bases
ATGATCGATCTCTATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGGGCTGGATTATCG
CTTGATAAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGCCCGGAATTTTTGCACATTTCGCCTAACAACAAAATTCCGG
CAATTGTTGATCATTCTCCAGCCGATGGCGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCT
GAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTTGGCGG
ACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATC
AGGTTGAAACTCAGCGTCTGTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGTGGCGAGAACTACAGC
ATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTCGCAATGTATCCGGCAGTCAA
GAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACACGGTGACGAGCGTTCGG
ATAGTTAA

Upstream 100 bases:

>100_bases
CTATAGAGCCTTTTTGTCACTGTCACGTGACGAATACTCACGCAAGTCGCCGCGCTTTCTATACTTGTGGTGTTCAGATG
ATTAGACAACCGGAGCTGCA

Downstream 100 bases:

>100_bases
CAGAAACAGGTTCTCGTGTATTATTTCATTCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATT
CGTATAAAGAACCTTCGTTT

Product: glutathione S-transferase

Products: NA

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFLHISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLA
EKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS

Sequences:

>Translated_215_residues
MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFLHISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLA
EKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS
>Mature_215_residues
MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFLHISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLA
EKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=215, Percent_Identity=98.6046511627907, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI87082195, Length=212, Percent_Identity=40.0943396226415, Blast_Score=152, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324100, Length=233, Percent_Identity=34.3347639484979, Blast_Score=123, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28571670, Length=176, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17864598, Length=205, Percent_Identity=27.8048780487805, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17864592, Length=199, Percent_Identity=25.6281407035176, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17864594, Length=193, Percent_Identity=25.3886010362694, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI85725204, Length=201, Percent_Identity=26.865671641791, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17737923, Length=201, Percent_Identity=26.865671641791, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45549270, Length=199, Percent_Identity=24.6231155778894, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24646249, Length=202, Percent_Identity=27.2277227722772, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17933730, Length=216, Percent_Identity=25.462962962963, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 24449; Mature: 24449

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFLHISPNNKIPAIVDHSP
CEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEEECCC
ADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNH
CCCCCCHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYSIADIACWPWVNAWTRQRIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHCCEEE
AMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS
EECHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCC
>Mature Secondary Structure
MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFLHISPNNKIPAIVDHSP
CEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEEECCC
ADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNH
CCCCCCHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYSIADIACWPWVNAWTRQRIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHCCEEE
AMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS
EECHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]