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The map label for this gene is atoS

Identifier: 218700688

GI number: 218700688

Start: 2432134

End: 2433960

Strand: Direct

Name: atoS

Synonym: ECIAI39_2357

Alternate gene names: 218700688

Gene position: 2432134-2433960 (Clockwise)

Preceding gene: 218700686

Following gene: 218700689

Centisome position: 47.39

GC content: 48.66

Gene sequence:

>1827_bases
ATGCATTATATGAAGTGGATTTATCCACGCCGCTTACGCAATCAAATGATTCTGATGGCAATCCTGATGGTCATTGTCCC
AACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCG
TGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCA
TTAAATGCAGAACTTGCCCCCATTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGCGCTGGTTATTACAACAAAAC
GCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTG
GTCGCGAAGTCATGCGTACAAATACCCCTTTGGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGATC
CCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTCGCCGCCAGGCCTGGAA
AATGGATGTGAGGATTATCATTGTGCTCACCGCCGGTTTGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCC
TGAGCGCCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCC
GGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGAT
TATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCGGCAGCAGAAGTCA
TTACTGGCTATCAACGTCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTA
CTGGATACGCTGGAACATGGCACCGAACATGTAGCACTGGAGATCAGTTTTCCAGGCCGTGACCGCACCATTGAACTCAG
TGTCACGACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCA
AAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCTACACTGGGTGAGCTGATGGCTGGCGTGGCGCATGAAGTA
CGTAATCCGCTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGCGACCTAATACATCAGGAATATCT
GTCCGTAGTACTCAAAGAAATTGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTC
AATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTC
GACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATAT
CCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGCATTCGAACCTGGCAATACAGCGACTCACAACAGG
CCATTTCGATAGAGGACAACGGCTGTGGCATTGATCTCTCGCTGCAAAAAAAGATCTTCGATCCCTTTTTCACCACCAAA
GCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTT
GCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGA

Upstream 100 bases:

>100_bases
GCAATGTTCTCTCTTCTCTGGAATATGATACACCGCCGAGAAATCATCACCTTAACCTCTGATAATCGTCATATACCGGA
CAAGACTAGTGGATTTCAGC

Downstream 100 bases:

>100_bases
CTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGA
TTCGAAACACATTGTGCGAA

Product: sensory histidine kinase AtoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVLSEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRA
LNAELAPITENITHAFPGIGAGYYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANIDIITDGLSTLAQNIPTRLPQLP
GEMGQISQSVNNLAQALRETRTLNDLIIENAADGVIAIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPV
LDTLEHGTEHVALEISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGELMAGVAHEV
RNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRPRHSQWQQVSLNALVEETLVLVQTAGVQARV
DFISELDNELSPINADRELLKQVLLNILINAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTK
ASGTGLGLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV

Sequences:

>Translated_608_residues
MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVLSEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRA
LNAELAPITENITHAFPGIGAGYYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANIDIITDGLSTLAQNIPTRLPQLP
GEMGQISQSVNNLAQALRETRTLNDLIIENAADGVIAIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPV
LDTLEHGTEHVALEISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGELMAGVAHEV
RNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRPRHSQWQQVSLNALVEETLVLVQTAGVQARV
DFISELDNELSPINADRELLKQVLLNILINAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTK
ASGTGLGLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV
>Mature_608_residues
MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVLSEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRA
LNAELAPITENITHAFPGIGAGYYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANIDIITDGLSTLAQNIPTRLPQLP
GEMGQISQSVNNLAQALRETRTLNDLIIENAADGVIAIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPV
LDTLEHGTEHVALEISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGELMAGVAHEV
RNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRPRHSQWQQVSLNALVEETLVLVQTAGVQARV
DFISELDNELSPINADRELLKQVLLNILINAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTK
ASGTGLGLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV

Specific function: Member of the two-component regulatory system AtoS/AtoC; may activate AtoC by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=608, Percent_Identity=99.3421052631579, Blast_Score=1225, Evalue=0.0,
Organism=Escherichia coli, GI1790436, Length=242, Percent_Identity=42.1487603305785, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1790300, Length=350, Percent_Identity=28.8571428571429, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1786600, Length=365, Percent_Identity=23.8356164383562, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693157, Length=273, Percent_Identity=29.6703296703297, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI48994928, Length=421, Percent_Identity=25.41567695962, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87081816, Length=267, Percent_Identity=27.7153558052434, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1786912, Length=311, Percent_Identity=27.3311897106109, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789808, Length=217, Percent_Identity=27.1889400921659, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788713, Length=367, Percent_Identity=23.9782016348774, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789149, Length=247, Percent_Identity=25.9109311740891, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788393, Length=264, Percent_Identity=25.3787878787879, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI87082128, Length=260, Percent_Identity=26.1538461538462, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788279, Length=225, Percent_Identity=24.8888888888889, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1790346, Length=234, Percent_Identity=26.9230769230769, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67805; Mature: 67805

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50885 HAMP ; PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVLSEKEKKLSAVVNLLNQ
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHH
ALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAGYYNKTLDAIITYAPSALY
HCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHH
QNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMIPIERNGEILGYIWANELTED
CCCCCEEEECCCCCHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHH
IRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANIDIITDGLSTLAQNIPTRLPQLP
HHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCC
GEMGQISQSVNNLAQALRETRTLNDLIIENAADGVIAIDRQGDVTTMNPAAEVITGYQRH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCHHHHHHCHHHH
ELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALEISFPGRDRTIELSVTTSRIHNTHGEM
HHCCCCHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEHHHHHHHHHHH
IGALVIFSDLTARKETQRRMAQAERLATLGELMAGVAHEVRNPLTAIRGYVQILRQQTSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIHQEYLSVVLKEIDSINKVIQQLLEFSRPRHSQWQQVSLNALVEETLVLVQTAGVQARV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
DFISELDNELSPINADRELLKQVLLNILINAVQAISARGKIRIRTWQYSDSQQAISIEDN
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEECC
GCGIDLSLQKKIFDPFFTTKASGTGLGLALSQRIINAHQGDIRVASLPGYGATFTLILPI
CCCEEEEHHHHHCCHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEE
NPQGNQTV
CCCCCCCC
>Mature Secondary Structure
MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVLSEKEKKLSAVVNLLNQ
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHH
ALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAGYYNKTLDAIITYAPSALY
HCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHH
QNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMIPIERNGEILGYIWANELTED
CCCCCEEEECCCCCHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHH
IRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANIDIITDGLSTLAQNIPTRLPQLP
HHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCC
GEMGQISQSVNNLAQALRETRTLNDLIIENAADGVIAIDRQGDVTTMNPAAEVITGYQRH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCHHHHHHCHHHH
ELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALEISFPGRDRTIELSVTTSRIHNTHGEM
HHCCCCHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEHHHHHHHHHHH
IGALVIFSDLTARKETQRRMAQAERLATLGELMAGVAHEVRNPLTAIRGYVQILRQQTSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIHQEYLSVVLKEIDSINKVIQQLLEFSRPRHSQWQQVSLNALVEETLVLVQTAGVQARV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
DFISELDNELSPINADRELLKQVLLNILINAVQAISARGKIRIRTWQYSDSQQAISIEDN
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEECC
GCGIDLSLQKKIFDPFFTTKASGTGLGLALSQRIINAHQGDIRVASLPGYGATFTLILPI
CCCEEEEHHHHHCCHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEE
NPQGNQTV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8346225; 9097040; 9278503 [H]