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The map label for this gene is ycaN

Identifier: 218700582

GI number: 218700582

Start: 2306055

End: 2306963

Strand: Direct

Name: ycaN

Synonym: ECIAI39_2248

Alternate gene names: 218700582

Gene position: 2306055-2306963 (Clockwise)

Preceding gene: 218700580

Following gene: 218700585

Centisome position: 44.93

GC content: 46.53

Gene sequence:

>909_bases
ATGCGGATGAATATGTCTGACTTTGCCACTTTCTTTGCCGTGGCCCGTAATCAGAGCTTTCGTGCAGCGGGCGATGAGTT
AGGCTTAACCTCGTCCGCCATTAGCCATAATATTAAAACACTGGAACAACGTCTTAAAATTCGCTTATTCAATCGGACTA
CCCGAAGCGTATCGTTAACGGAAGCGGGTTCTAATCTCTATGAACGATTGCGCCCAGCTTTCGACGAAATTCAGATCATG
TTAGATGAGATGAATGATTTTCGTCTGACACCGACAGGTACGCTCAAAATCAATGCCGCCCGGGTAGCTGCACGTATATT
TTTAATGCCTTTACTGGTTGGTTTCACACGTGAATATCCTGATATTAAAGTCGAGCTCACAACTGACGACTCTCTTGTTG
ATATCGTCCAGCAGGGCTTTGACGCTGGCGTTCGACTTAGTGGCATCGTCGAAAAAGATATGATCAGCGTGGCGATCGGC
CCGCCAGTGAAATTATGTGTAGCAGCAACGCCAGAGTACTTTGCAAGATATGGTAAACCCCGCCATCCTCATGATCTGCT
TAATCATCAATGTGTCGTTTTTCGTTATCCCAGCGGCAAACCTTTCCACTGGCAATTTGCAAAGGAGCTGAAAATTGCTG
TCGCAGGAAATATCATTCTGGATGATGTTGATGCTGAACTGGATGCCGTTTTGATGGGTGCTGGTATCGGGTATTTGCTC
TATGAACAGATCAAGGAGTACCTTGATACCGGTCGTTTAGAATGTGTACTGGAAGACTGGATCACAGAGCGTCCAGGCTT
CCAGATCTATTATCCAAACCGCCAGTATATGTCCTGCGGCTTGCGCGCATTTCTCGATTATGTAAAAACGGGTCAGATCT
GCCAGAGTCAGCGTCACCGACCACAATAA

Upstream 100 bases:

>100_bases
TAATTTTACCTCCACGTTAAGTTCTGGAGCGCACTTTACGCAGGCGGCATAATCCTGTAAACGTGATGATTCAGGATGAG
TTAATGAAGGATTTTCACTA

Downstream 100 bases:

>100_bases
TTATTTACTATTCGCTTTACGCGCCAGCAGCGGGAATATCAGCCCTAATCCTACCAGCACAAACGGCGTTGCAACATTCA
GCGCCAACTGGAAGGTCCAC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRMNMSDFATFFAVARNQSFRAAGDELGLTSSAISHNIKTLEQRLKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIM
LDEMNDFRLTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELKIAVAGNIILDDVDAELDAVLMGAGIGYLL
YEQIKEYLDTGRLECVLEDWITERPGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ

Sequences:

>Translated_302_residues
MRMNMSDFATFFAVARNQSFRAAGDELGLTSSAISHNIKTLEQRLKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIM
LDEMNDFRLTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELKIAVAGNIILDDVDAELDAVLMGAGIGYLL
YEQIKEYLDTGRLECVLEDWITERPGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ
>Mature_302_residues
MRMNMSDFATFFAVARNQSFRAAGDELGLTSSAISHNIKTLEQRLKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIM
LDEMNDFRLTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELKIAVAGNIILDDVDAELDAVLMGAGIGYLL
YEQIKEYLDTGRLECVLEDWITERPGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=302, Percent_Identity=97.682119205298, Blast_Score=615, Evalue=1e-178,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=34.2465753424658, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=29.3918918918919, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=28.0276816608997, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1789639, Length=304, Percent_Identity=26.9736842105263, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI145693193, Length=282, Percent_Identity=28.7234042553192, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1789440, Length=298, Percent_Identity=24.496644295302, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788706, Length=279, Percent_Identity=27.5985663082437, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786448, Length=297, Percent_Identity=28.956228956229, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789173, Length=306, Percent_Identity=24.8366013071895, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI145693105, Length=161, Percent_Identity=29.8136645962733, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788748, Length=123, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34311; Mature: 34311

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMNMSDFATFFAVARNQSFRAAGDELGLTSSAISHNIKTLEQRLKIRLFNRTTRSVSLT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEH
EAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARVAARIFLMPLLVGFTREYP
HHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
DIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKP
CEEEEEECCHHHHHHHHHCCCCCCEEECCHHHHHEEEECCCCEEEEEECCHHHHHHCCCC
RHPHDLLNHQCVVFRYPSGKPFHWQFAKELKIAVAGNIILDDVDAELDAVLMGAGIGYLL
CCCHHHHCCCEEEEECCCCCCEEEEHHHHEEEEEECCEEEECCCCHHHHHHHHCCHHHHH
YEQIKEYLDTGRLECVLEDWITERPGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHR
HHHHHHHHCCCCCEEHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHCCC
PQ
CC
>Mature Secondary Structure
MRMNMSDFATFFAVARNQSFRAAGDELGLTSSAISHNIKTLEQRLKIRLFNRTTRSVSLT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEH
EAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARVAARIFLMPLLVGFTREYP
HHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
DIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKP
CEEEEEECCHHHHHHHHHCCCCCCEEECCHHHHHEEEECCCCEEEEEECCHHHHHHCCCC
RHPHDLLNHQCVVFRYPSGKPFHWQFAKELKIAVAGNIILDDVDAELDAVLMGAGIGYLL
CCCHHHHCCCEEEEECCCCCCEEEEHHHHEEEEEECCEEEECCCCHHHHHHHHCCHHHHH
YEQIKEYLDTGRLECVLEDWITERPGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHR
HHHHHHHHCCCCCEEHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHCCC
PQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]