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The map label for this gene is pflA

Identifier: 218700580

GI number: 218700580

Start: 2304543

End: 2305283

Strand: Direct

Name: pflA

Synonym: ECIAI39_2246

Alternate gene names: 218700580

Gene position: 2304543-2305283 (Clockwise)

Preceding gene: 218700579

Following gene: 218700582

Centisome position: 44.9

GC content: 49.8

Gene sequence:

>741_bases
ATGTCAGTTATTGGTCGCATTCACTCCTTTGAATCCTGTGGAACCGTAGACGGCCCGGGTATTCGCTTTATCACCTTTTT
CCAGGGCTGCCTGATGCGCTGCCTGTATTGTCATAACCGCGACACCTGGGACACGCATGGCGGTAAAGAAGTTACCGTTG
AAGATTTGATGAAGGAAGTGGTGACCTATCGCCACTTTATGAACGCTTCCGGCGGCGGCGTTACCGCATCCGGCGGTGAA
GCAATCCTGCAAGCTGAGTTTGTTCGTGACTGGTTCCGCGCCTGCAAAAAAGAAGGCATTCATACCTGTCTGGATACCAA
CGGTTTTGTTCGTCGTTACGATCCGGTGATTGATGAACTGCTGGAAGTAACCGACCTGGTAATGCTCGATCTCAAACAGA
TGAACGACGAGATCCACCAAAATCTGGTTGGAGTTTCCAACCACCGCACGCTGGAGTTTGCTAAATATCTGGCGAATAAA
AATGTGAAGGTGTGGATCCGCTACGTTGTTGTCCCAGGCTGGTCTGACGATGACGATTCAGCGCATCGCCTCGGTGAATT
TACCCGTGATATGGGCAACGTTGAGAAAATCGAGCTTCTCCCCTACCACGAGCTGGGCAAACACAAATGGGTGGCGATGG
GTGAAGAATACAAACTTGATGGTGTTAAACCACCGAAGAAAGAGACCATGGAACGCGTGAAAGGCATTCTTGAGCAGTAC
GGTCATAAGGTAATGTTCTAA

Upstream 100 bases:

>100_bases
CTATCTATACTTTAAGGTGACTGCCAAAACAGACTCGACGTAGCCTTCGAGCTGCGCACCAACACGGCCTCAGATGGGCC
ACATCTGGAGAAACACCGCA

Downstream 100 bases:

>100_bases
TTTCCCCGTAAAGCGGCAACTTTATTGAGTTGCCGCTTTGTTATTCCGCGCTTCATTCTGCTACAGCGTTTTTAACACAT
CTTATAGTGCATCTACCATA

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGE
AILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETMERVKGILEQY
GHKVMF

Sequences:

>Translated_246_residues
MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGE
AILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETMERVKGILEQY
GHKVMF
>Mature_245_residues
SVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEA
ILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANKN
VKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETMERVKGILEQYG
HKVMF

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=100, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI1790389, Length=274, Percent_Identity=25.9124087591241, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790839, Length=287, Percent_Identity=27.1777003484321, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI226510931, Length=171, Percent_Identity=32.7485380116959, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PFLA_ECO57 (P0A9N6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A99752
- PIR:   G85615
- RefSeq:   NP_286777.1
- RefSeq:   NP_309012.1
- ProteinModelPortal:   P0A9N6
- SMR:   P0A9N6
- EnsemblBacteria:   EBESCT00000025924
- EnsemblBacteria:   EBESCT00000055714
- GeneID:   917728
- GeneID:   958863
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1246
- KEGG:   ecs:ECs0985
- GeneTree:   EBGT00050000009897
- HOGENOM:   HBG554871
- OMA:   ETCGTVD
- ProtClustDB:   PRK11145
- BioCyc:   ECOL83334:ECS0985-MONOMER
- GO:   GO:0005737
- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197
- SMART:   SM00729
- TIGRFAMs:   TIGR02493

Pfam domain/function: PF04055 Radical_SAM

EC number: =1.97.1.4

Molecular weight: Translated: 28205; Mature: 28073

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEV
CCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHH
VTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANKNVKVWIRYVVVPGWSDDDDS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCHH
AHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETMERVKGILEQY
HHHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCEECCCCCCCHHHHHHHHHHHHHHH
GHKVMF
CCCCCC
>Mature Secondary Structure 
SVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEV
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHH
VTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANKNVKVWIRYVVVPGWSDDDDS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCHH
AHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETMERVKGILEQY
HHHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCEECCCCCCCHHHHHHHHHHHHHHH
GHKVMF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796