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The map label for this gene is nemA

Identifier: 218699782

GI number: 218699782

Start: 1479489

End: 1480586

Strand: Reverse

Name: nemA

Synonym: ECIAI39_1406

Alternate gene names: 218699782

Gene position: 1480586-1479489 (Counterclockwise)

Preceding gene: 218699783

Following gene: 218699781

Centisome position: 28.85

GC content: 53.64

Gene sequence:

>1098_bases
ATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGAC
GCGTCTTCGCAGTATTGAACCGGGTGACATCCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTT
TGATTATTAGCGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGGTATGCTGGTGCACCTGGCATCCATAGTCCGGAACAA
ATTGCCGCATGGAAAAAAATTACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTACAGCTGTGGCACACCGGACG
CATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCCGCACTTAGCGCGGGAACACGTACTTCTC
TGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCGGGTATC
GTCAATGATTTCCGTCAGGCCATTTCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGCTCCACTCTGCTCATGGTTA
TTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTC
TGGTGCTGGAAGTGGTCGATGCAGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCGCCAATCGGTACT
TTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGC
TTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAGCCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTT
TCCACGGTCCGATTATCGGCGCAGGTGCATACACGGTAGAAAAAGCCGAAACGCTGATCGGCAAAGGGTTAATTGATGCG
GTGGCATTTGGTCGTGACTGGATTGCAAACCCGGATCTGGTCGCCCGCTTGCAACGCAAAGCTGAGCTTAACCCACAGCG
TGCCGAAAGTTTTTACGGTGGCGGCGCGGAAGGCTACACGGATTACCCGACGCTGTAA

Upstream 100 bases:

>100_bases
TTCCGAGCCACTGGAAAACGCGCTGGCACATGTAAAAACCATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCA
CCAGACGACCGGGAGCCTTT

Downstream 100 bases:

>100_bases
TCCAACATTGCGAGCGGCGCAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATATAAAG
ATGCGTCTTCTTCATACCAT

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQ
IAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
VNDFRQAISNAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDA
VAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL

Sequences:

>Translated_365_residues
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQ
IAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
VNDFRQAISNAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDA
VAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL
>Mature_364_residues
SSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQI
AAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIV
NDFRQAISNAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGTF
QNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAV
AFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=99.7260273972603, Blast_Score=742, Evalue=0.0,
Organism=Escherichia coli, GI1789463, Length=359, Percent_Identity=31.4763231197772, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=356, Percent_Identity=30.3370786516854, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=350, Percent_Identity=28.5714285714286, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=268, Percent_Identity=29.8507462686567, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=351, Percent_Identity=27.0655270655271, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=374, Percent_Identity=28.6096256684492, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=354, Percent_Identity=30.225988700565, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=216, Percent_Identity=31.0185185185185, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=389, Percent_Identity=39.0745501285347, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6321973, Length=389, Percent_Identity=37.2750642673522, Blast_Score=219, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39533; Mature: 39402

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIVNDFRQAISNAREAGFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCEECHHHCCCHHHHHHHHHHHHHHCCCCEEE
LHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
HHCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIG
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
DYPTL
CCCCC
>Mature Secondary Structure 
SSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIVNDFRQAISNAREAGFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCEECHHHCCCHHHHHHHHHHHHHHCCCCEEE
LHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
HHCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIG
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
DYPTL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]