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The map label for this gene is lpfB

Identifier: 218697262

GI number: 218697262

Start: 4081164

End: 4081850

Strand: Reverse

Name: lpfB

Synonym: EC55989_4001

Alternate gene names: 218697262

Gene position: 4081850-4081164 (Counterclockwise)

Preceding gene: 218697263

Following gene: 218697261

Centisome position: 79.18

GC content: 41.05

Gene sequence:

>687_bases
ATGAAACGTATTAGCTTTGCTGTGCTGGCACTTTTAATGACCGCACAACAGTGTTTTGCTGGTGGCGTAGCCTTAAGCAC
GACGCGTGTTATTTATGATGGCGGCAAGAAAGAAGCGTCGCTGACCGTTGAAAACCATAATAAAAATGAAGAGTTTTTGA
TCCAGTCATGGATTGATGATGTGAATGGCAATAAAAAAACACCGTTTATTATTACACCGCCATTATTCAAACTTGATCCT
GATAAAAATAACGTCTTACGTATCGTCAATATTGAAGATACCTTACCTAAAGATCGTGAATCCGTTTACTGGATTAATGT
AAAGGCCATTCCTGCGAAAAGTGCTGAAAATGAAAATAAAAACGTCTTACAGATAGCCGTTCGCACCCGTTTGAAGTTGT
TCTATCGTCCAAAAGGTTTGCCGGGAAGCAACCTCGAGGCCATGAAACAGTTACGTTTCCAGCGTCAAAATAACCAAATT
GTGGTTAATAACGACTCCGCTTATAACCTGACCTTTAATCAGTTTTCCATTAATGGACAAAAGATTGAACGGGCGGGAAT
GGTTCTCGCGAAAGGAAAGTTAAACATTAACCTTCCAGCCGGTACAGGCAACGCGCACGAAGTGAAATACAGCATTATTA
ATGATTTCGGTGTTGCTGGCGAAATGCTGACGAAGCCGATTAACTGA

Upstream 100 bases:

>100_bases
ACCTGACTTACAACTAATCGATCCTGACGGAACGATCATCTCTCAATGGCAACAGAGACTGTGTTGCCATTATTCACACC
AATGACTCAGGGACCGAATC

Downstream 100 bases:

>100_bases
TCCTTTTACAAGGCGCTTTTACTATGCATTGGATGCATTTACCTCTCTATCACTACAGAGCGCATTTTTCCTTCTCTCTG
CTGGCATTAACGATTGCCAG

Product: Chaperone protein lpfB precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVENHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDP
DKNNVLRIVNIEDTLPKDRESVYWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGVAGEMLTKPIN

Sequences:

>Translated_228_residues
MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVENHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDP
DKNNVLRIVNIEDTLPKDRESVYWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGVAGEMLTKPIN
>Mature_228_residues
MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVENHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDP
DKNNVLRIVNIEDTLPKDRESVYWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGVAGEMLTKPIN

Specific function: Required for the biogenesis of long polar fimbria; binds and interact with lpfA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1789532, Length=231, Percent_Identity=37.2294372294372, Blast_Score=172, Evalue=1e-44,
Organism=Escherichia coli, GI1790771, Length=229, Percent_Identity=38.8646288209607, Blast_Score=162, Evalue=1e-41,
Organism=Escherichia coli, GI1786743, Length=219, Percent_Identity=38.3561643835616, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1787171, Length=217, Percent_Identity=36.405529953917, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788428, Length=219, Percent_Identity=30.5936073059361, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786333, Length=235, Percent_Identity=33.1914893617021, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87081806, Length=220, Percent_Identity=31.3636363636364, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1786936, Length=217, Percent_Identity=29.4930875576037, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI87082203, Length=223, Percent_Identity=32.2869955156951, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788677, Length=202, Percent_Identity=32.6732673267327, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 25517; Mature: 25517

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVENHNKNEEFLIQSWIDD
CCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
VNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESVYWINVKAIPAKSAENENK
CCCCCCCCEEECCCCEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCC
NVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQIVVNNDSAYNLTFNQFSINGQ
CEEEEEEEHHEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCC
KIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGVAGEMLTKPIN
EEHCCCEEEEECEEEEEECCCCCCCCEEEEEEECCCCCCCHHHCCCCC
>Mature Secondary Structure
MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVENHNKNEEFLIQSWIDD
CCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
VNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESVYWINVKAIPAKSAENENK
CCCCCCCCEEECCCCEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCC
NVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQIVVNNDSAYNLTFNQFSINGQ
CEEEEEEEHHEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCC
KIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGVAGEMLTKPIN
EEHCCCEEEEECEEEEEECCCCCCCCEEEEEEECCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7721701; 11677609 [H]