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The map label for this gene is epsK [H]

Identifier: 218696666

GI number: 218696666

Start: 3452212

End: 3453189

Strand: Reverse

Name: epsK [H]

Synonym: EC55989_3371

Alternate gene names: 218696666

Gene position: 3453189-3452212 (Counterclockwise)

Preceding gene: 218696667

Following gene: 218696665

Centisome position: 66.99

GC content: 56.03

Gene sequence:

>978_bases
ATGATCACCTCACCACCAAAACGCGGAATGGCACTGGTCGTGGTGCTGGTATTGCTGGCAGTTATGATGCTGGTAACCAT
CACGCTTTCCGGGCGGATGCAGCAGCAACTGGGAAGAACGCGCAGCCAGCAGGAGTACCAGCAGGCGCTGTGGTACAGCG
CCAGTGCAGAAAGCCTGGCGCTGAGCGCGCTCAGTCTGAGCCTGAAAAATGAAAAGCGCGTGCATCTGGAACAGCCGTGG
GCTTCCGGCCCTCGTTTTTTCCCACTGCCGCAGGGGCAAATCGCCGTCACTCTGCGTGACGCACAGGCCTGCTTTAACCT
GAATGCCCTCGCTCAGCCCACAACGGCGTCGCGTCCGCTCGCGGTACAACAACTGATTGCCCTGATCACGCGCCTGGATG
TGCCTGCTTATCGGGCCGAACTGATAGCCGAAAGCCTGTGGGAGTTTATTGATGAAGACCGCAGCGTGCAGACGCGTCTG
GGCCGTGAAGACAGCGAATATCTCGCCCGCTCGGTGCCTTTCTACGCCGCCAATCAACCGCTGGCCGATATCAGCGAGAT
GCGCGTGGTGCAGGGAATGGACGCCGGGCTTTATCAAAAACTGAAACCGCTGGTCTGTGCGCTGCCGATGACCCGCCAGC
AAATCAACATCAATACTTTAGACGTCACGCAAAGTGTGATTCTTGAGGCGCTGTTTGACCCGTGGTTAAGCCCTGTTCAG
GCGCGGGCGTTATTACAACAACGTCCGGCGAAGGGCTGGGAAGATGTCGATCAGTTTCTTGCACAGCCGCTACTTGCTGA
CGTCGATGAGCGTACTAAAAAACAGCTAAAAACCGTCCTGAGCGTGGACAGCAATTACTTCTGGCTGCGTTCAGATATCA
CCGTGAATGAGATTGAACTGACGATGAACTCGTTAATTGTCCGCATGGGCCCACAACACTTTTCGGTTCTCTGGCATCAG
ACAGGAGAAAGTGAGTGA

Upstream 100 bases:

>100_bases
CGTCAGTACAGGCGATCCCTGTGGCGGTACGCATGACCCTGCATTCGCCGCAATGGGGCGAGATTGAACGCATCTGGTTG
TTACGCGGGCCGCAATAATC

Downstream 100 bases:

>100_bases
GTTCCATCCTTGAGATTTTTTTCCCGCTTTGCGCCGCTGATCCCATCCATTGGCAGCGCCGTACACCCGATGTGGAGCAC
GGTATCTGGTCTGACGTTGC

Product: putative type II secretion protein (GspK-like)

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLEQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE

Sequences:

>Translated_325_residues
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLEQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE
>Mature_325_residues
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLEQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=285, Percent_Identity=35.0877192982456, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 36601; Mature: 36601

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
LSALSLSLKNEKRVHLEQPWASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPL
HHHHHHHHCCCCCEEECCCCCCCCEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCH
AVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQP
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCC
LADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININTLDVTQSVILEALFDPWLSPVQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHH
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
TMNSLIVRMGPQHFSVLWHQTGESE
HHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
LSALSLSLKNEKRVHLEQPWASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPL
HHHHHHHHCCCCCEEECCCCCCCCEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCH
AVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQP
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCC
LADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININTLDVTQSVILEALFDPWLSPVQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHH
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
TMNSLIVRMGPQHFSVLWHQTGESE
HHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]