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The map label for this gene is kpnIM [H]

Identifier: 218696572

GI number: 218696572

Start: 3349849

End: 3351477

Strand: Direct

Name: kpnIM [H]

Synonym: EC55989_3268

Alternate gene names: 218696572

Gene position: 3349849-3351477 (Clockwise)

Preceding gene: 218696571

Following gene: 218696573

Centisome position: 64.98

GC content: 41.74

Gene sequence:

>1629_bases
ATGTCAACGAAGCAAAAATTAGAACTTAACTGGATTGGTAAGCATAAGCGTCCTCGTCTGGAGCCGCGTATTCTGCTCGA
AGATAAGGAGTTATCTTATGGTGATGCGGATAGTGAGAATCTGCTGATTCATGGGGATAATCTACTGGCGTTGAAGGCAC
TGGAGCAGAAGTATGCGGGGAAGGTGAAGTGTATATTTATCGACCCTCCTTATAATACTGGAAGTGCTTTTACACATTAC
GATGATGGACTTGAACACTCTCTATGGCTTTGCTTGATCAGAGATCGGTTAGAATTATTACGCACTTTATTATCTGAAGA
TGGCTCTATATGGATAACAATTGATGACCACGAAGTTCATTATTTAAAATGTCTTTGTGATGAAATATTCGGAAGGAATA
ACTTTATTTCAAATGTTATTTGGGAAAAATCCGATAGCCCAAGAATGGATGCTGCATTTTTCTCTTCTCGCCATGATCAC
ATATTAGTGTACGCGAAAGATCAGTCAAAATTCAAAGTAAAAAGACAACCACTGTCCCTCAATGATCTACCTAGCCATTA
CGATAAAGTAGATCCAAGCGGCAATCCTTACTACCTAAAACCGCTAAGGGCAATGGGACAAGCAGATAAAAGAGAAGATC
GCCCAACAATGTATTTCCCTCTTGTTGCCCCCGATGGCACAGAAATTTTTCCAAAGCGCCAAGATGGAACTGATGGACGT
TGGCGTTGGGGCGTACAAAAAATAGATCAGGAGAAATGGAGAATTGATTGGTCGAAAGGACGAAATGGTTGGACTCCATA
TTTTCGTGTATATGCTGATAGTTCGTCAGGTAGACCGCCAGAAACGATATGGTTTCATTCTGAAGTTGGAAGTAACAGGA
CATCAAAAGCCGAAATTAAGAAGGTGATTCCTACTATAAGTGCATTTGATACACCTAAGCCGGAAGCTCTTATAAAAAAA
ATACTGGAGATTGCAACTGACAATGGTGATCTTGTTCTAGACTCCTTTGCTGGTTCAGGCACAACAGGGGCCGTAGCACA
AAAGATGGGGCGTCAGTGGATTATGGTTGAATTAGGCGACCACTGTCGGACTCATATTGTTCCGCGTATGAAAAAAGTGA
TTGATGGGAAAGATTTAGGCGGTATTACAAAATCTGTAAGCTGGCAAGGTGGTGGAGGTTTTCGTTATTTGAGTCTAGCA
CCTTCATTGCTTAAAAAAGATACTTGGGGTAATTGGGTAATAAATAAATATTACAATCCTGAAATGCTTGCTGAAGCCAT
GTGCAAACATATGAACTTCACCTATGCACCAAGCCAGACTCAATACTGGAATCACGGTTACAGCACCGAAACTGACCACA
TCTACGTCACTACCGGTTCTTTGGCTTATGAACAACTGAAAGTTCTGAGTGAAGAGGTAGGAACTGAACGTACATTACTG
ATTTGCTGCAAAGCCTTTATGACTGAAGGCGCGGACTTCCCTAACCTGACGCTGGTAAAAATTCCGCGTGCCATTTTGAG
CAAATGCGAATGGGATCAGGACGACTATAGCTTTACGCTAAACGTTCTCTCTGATTCAGAACAGCCTGACGATATTGACT
ACGACGAAGACACCGAAGACGAAGAATAA

Upstream 100 bases:

>100_bases
GACCGATCGCGATAATGAACAGGCGCAGCTTTATACACAAATTCAGGCGCAGATCCGTGAACAGGAACGCGCACGAGCAC
GTATTGGATAAAAATAGATT

Downstream 100 bases:

>100_bases
TTATGAGCCAGAATAACGCCGTTAAAATTGTCAATCGAGTTAGCGCACGACTGTCTCTGCGCGATCCGCAGGATGAATCA
TTGTGCATCTTATGTGACGT

Product: DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.KpnI; Adenine-specific methyltransferase KpnI [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MSTKQKLELNWIGKHKRPRLEPRILLEDKELSYGDADSENLLIHGDNLLALKALEQKYAGKVKCIFIDPPYNTGSAFTHY
DDGLEHSLWLCLIRDRLELLRTLLSEDGSIWITIDDHEVHYLKCLCDEIFGRNNFISNVIWEKSDSPRMDAAFFSSRHDH
ILVYAKDQSKFKVKRQPLSLNDLPSHYDKVDPSGNPYYLKPLRAMGQADKREDRPTMYFPLVAPDGTEIFPKRQDGTDGR
WRWGVQKIDQEKWRIDWSKGRNGWTPYFRVYADSSSGRPPETIWFHSEVGSNRTSKAEIKKVIPTISAFDTPKPEALIKK
ILEIATDNGDLVLDSFAGSGTTGAVAQKMGRQWIMVELGDHCRTHIVPRMKKVIDGKDLGGITKSVSWQGGGGFRYLSLA
PSLLKKDTWGNWVINKYYNPEMLAEAMCKHMNFTYAPSQTQYWNHGYSTETDHIYVTTGSLAYEQLKVLSEEVGTERTLL
ICCKAFMTEGADFPNLTLVKIPRAILSKCEWDQDDYSFTLNVLSDSEQPDDIDYDEDTEDEE

Sequences:

>Translated_542_residues
MSTKQKLELNWIGKHKRPRLEPRILLEDKELSYGDADSENLLIHGDNLLALKALEQKYAGKVKCIFIDPPYNTGSAFTHY
DDGLEHSLWLCLIRDRLELLRTLLSEDGSIWITIDDHEVHYLKCLCDEIFGRNNFISNVIWEKSDSPRMDAAFFSSRHDH
ILVYAKDQSKFKVKRQPLSLNDLPSHYDKVDPSGNPYYLKPLRAMGQADKREDRPTMYFPLVAPDGTEIFPKRQDGTDGR
WRWGVQKIDQEKWRIDWSKGRNGWTPYFRVYADSSSGRPPETIWFHSEVGSNRTSKAEIKKVIPTISAFDTPKPEALIKK
ILEIATDNGDLVLDSFAGSGTTGAVAQKMGRQWIMVELGDHCRTHIVPRMKKVIDGKDLGGITKSVSWQGGGGFRYLSLA
PSLLKKDTWGNWVINKYYNPEMLAEAMCKHMNFTYAPSQTQYWNHGYSTETDHIYVTTGSLAYEQLKVLSEEVGTERTLL
ICCKAFMTEGADFPNLTLVKIPRAILSKCEWDQDDYSFTLNVLSDSEQPDDIDYDEDTEDEE
>Mature_541_residues
STKQKLELNWIGKHKRPRLEPRILLEDKELSYGDADSENLLIHGDNLLALKALEQKYAGKVKCIFIDPPYNTGSAFTHYD
DGLEHSLWLCLIRDRLELLRTLLSEDGSIWITIDDHEVHYLKCLCDEIFGRNNFISNVIWEKSDSPRMDAAFFSSRHDHI
LVYAKDQSKFKVKRQPLSLNDLPSHYDKVDPSGNPYYLKPLRAMGQADKREDRPTMYFPLVAPDGTEIFPKRQDGTDGRW
RWGVQKIDQEKWRIDWSKGRNGWTPYFRVYADSSSGRPPETIWFHSEVGSNRTSKAEIKKVIPTISAFDTPKPEALIKKI
LEIATDNGDLVLDSFAGSGTTGAVAQKMGRQWIMVELGDHCRTHIVPRMKKVIDGKDLGGITKSVSWQGGGGFRYLSLAP
SLLKKDTWGNWVINKYYNPEMLAEAMCKHMNFTYAPSQTQYWNHGYSTETDHIYVTTGSLAYEQLKVLSEEVGTERTLLI
CCKAFMTEGADFPNLTLVKIPRAILSKCEWDQDDYSFTLNVLSDSEQPDDIDYDEDTEDEE

Specific function: This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 62108; Mature: 61977

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKQKLELNWIGKHKRPRLEPRILLEDKELSYGDADSENLLIHGDNLLALKALEQKYAG
CCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEHHHHHHHHCC
KVKCIFIDPPYNTGSAFTHYDDGLEHSLWLCLIRDRLELLRTLLSEDGSIWITIDDHEVH
CEEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
YLKCLCDEIFGRNNFISNVIWEKSDSPRMDAAFFSSRHDHILVYAKDQSKFKVKRQPLSL
HHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHCCCCCEEEEEECCCCCEEEECCCCCC
NDLPSHYDKVDPSGNPYYLKPLRAMGQADKREDRPTMYFPLVAPDGTEIFPKRQDGTDGR
CCCCCHHHCCCCCCCCEEECHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
WRWGVQKIDQEKWRIDWSKGRNGWTPYFRVYADSSSGRPPETIWFHSEVGSNRTSKAEIK
EECCHHHHCCHHEEEECCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHH
KVIPTISAFDTPKPEALIKKILEIATDNGDLVLDSFAGSGTTGAVAQKMGRQWIMVELGD
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHCCCEEEEEEECC
HCRTHIVPRMKKVIDGKDLGGITKSVSWQGGGGFRYLSLAPSLLKKDTWGNWVINKYYNP
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEHHHCHHHHHCCCCCCEEEECCCCH
EMLAEAMCKHMNFTYAPSQTQYWNHGYSTETDHIYVTTGSLAYEQLKVLSEEVGTERTLL
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHH
ICCKAFMTEGADFPNLTLVKIPRAILSKCEWDQDDYSFTLNVLSDSEQPDDIDYDEDTED
HHHHHHHHCCCCCCCEEHEECCHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
EE
CC
>Mature Secondary Structure 
STKQKLELNWIGKHKRPRLEPRILLEDKELSYGDADSENLLIHGDNLLALKALEQKYAG
CCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEHHHHHHHHCC
KVKCIFIDPPYNTGSAFTHYDDGLEHSLWLCLIRDRLELLRTLLSEDGSIWITIDDHEVH
CEEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
YLKCLCDEIFGRNNFISNVIWEKSDSPRMDAAFFSSRHDHILVYAKDQSKFKVKRQPLSL
HHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHCCCCCEEEEEECCCCCEEEECCCCCC
NDLPSHYDKVDPSGNPYYLKPLRAMGQADKREDRPTMYFPLVAPDGTEIFPKRQDGTDGR
CCCCCHHHCCCCCCCCEEECHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
WRWGVQKIDQEKWRIDWSKGRNGWTPYFRVYADSSSGRPPETIWFHSEVGSNRTSKAEIK
EECCHHHHCCHHEEEECCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHH
KVIPTISAFDTPKPEALIKKILEIATDNGDLVLDSFAGSGTTGAVAQKMGRQWIMVELGD
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHCCCEEEEEEECC
HCRTHIVPRMKKVIDGKDLGGITKSVSWQGGGGFRYLSLAPSLLKKDTWGNWVINKYYNP
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEHHHCHHHHHCCCCCCEEEECCCCH
EMLAEAMCKHMNFTYAPSQTQYWNHGYSTETDHIYVTTGSLAYEQLKVLSEEVGTERTLL
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHH
ICCKAFMTEGADFPNLTLVKIPRAILSKCEWDQDDYSFTLNVLSDSEQPDDIDYDEDTED
HHHHHHHHCCCCCCCEEHEECCHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1754388 [H]