Click here to switch to the map view.

The map label for this gene is ygcM

Identifier: 218696364

GI number: 218696364

Start: 3108202

End: 3108567

Strand: Direct

Name: ygcM

Synonym: EC55989_3039

Alternate gene names: 218696364

Gene position: 3108202-3108567 (Clockwise)

Preceding gene: 218696351

Following gene: 218696365

Centisome position: 60.3

GC content: 49.18

Gene sequence:

>366_bases
ATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATG
TGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCG
ATTTCGCTGAACTGAAAGCGGCGTTTAAGCCAACTTATGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTG
GAAAACCCAACCAGCGAAGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGAT
GGTAAAAGAAACCTGCACCGCTGGTTGTATCTATCGCGGCGAATGA

Upstream 100 bases:

>100_bases
AGATAAATCAGCGATTTTGCGCAACAAAGTCGTTTTAGATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTT
TATCGTCCTGTAGAGAAATT

Downstream 100 bases:

>100_bases
TAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATG
GAAGACGACTGCGACATTAT

Product: 6-pyruvoyl tetrahydrobiopterin synthase (PTPS)

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD

Number of amino acids: Translated: 121; Mature: 121

Protein sequence:

>121_residues
MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEVDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGL
ENPTSEVLAKWIWDQVKPVVPLLSAVMVKETCTAGCIYRGE

Sequences:

>Translated_121_residues
MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEVDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGL
ENPTSEVLAKWIWDQVKPVVPLLSAVMVKETCTAGCIYRGE
>Mature_121_residues
MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEVDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGL
ENPTSEVLAKWIWDQVKPVVPLLSAVMVKETCTAGCIYRGE

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily

Homologues:

Organism=Escherichia coli, GI1789124, Length=121, Percent_Identity=100, Blast_Score=254, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUED_ECO57 (P65871)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91081
- PIR:   E85926
- RefSeq:   NP_289315.1
- RefSeq:   NP_311647.1
- ProteinModelPortal:   P65871
- SMR:   P65871
- EnsemblBacteria:   EBESCT00000027243
- EnsemblBacteria:   EBESCT00000058938
- GeneID:   914658
- GeneID:   961174
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4075
- KEGG:   ecs:ECs3620
- GeneTree:   EBGT00050000009585
- HOGENOM:   HBG727155
- OMA:   IHETCTS
- ProtClustDB:   CLSK880481
- BioCyc:   ECOL83334:ECS3620-MONOMER
- InterPro:   IPR007116
- InterPro:   IPR007115
- PANTHER:   PTHR12589
- TIGRFAMs:   TIGR00039

Pfam domain/function: PF01242 PTPS

EC number: 4.2.3.12

Molecular weight: Translated: 13773; Mature: 13773

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: ACT_SITE 27-27 ACT_SITE 71-71 ACT_SITE 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEVDPHTGWIIDFAELKA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCEEEEEEEEECCCCCCCCEEEEHHHHHH
AFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLLSAVMVKETCTAGCIYRG
HHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
E
C
>Mature Secondary Structure
MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEVDPHTGWIIDFAELKA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCEEEEEEEEECCCCCCCCEEEEHHHHHH
AFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLLSAVMVKETCTAGCIYRG
HHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796