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The map label for this gene is hyfG

Identifier: 218696114

GI number: 218696114

Start: 2832356

End: 2834023

Strand: Direct

Name: hyfG

Synonym: EC55989_2772

Alternate gene names: 218696114

Gene position: 2832356-2834023 (Clockwise)

Preceding gene: 218696113

Following gene: 218696115

Centisome position: 54.95

GC content: 54.62

Gene sequence:

>1668_bases
CTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAA
TCTGCTGCCTGACGTTGTACATTATCTTTATTATCAACATGATGGCTGGCTTCCAGTCCTGTTTGGCAACGACGAGCGGA
CACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATCGTGGTGAAGGCACTG
GTCGATGCCGACAGTCGGGAGTTTCCGTCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGA
TATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGAAGATATGCATC
CGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACAGATTCCGAAACGTATGCGTTTATCAATGAG
GGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGT
GGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGA
TGGGCTACAACGAAGTGACCTTCTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAAC
TCGGTTGAAAATGCACTGGGGATTGAGGTGTCGCAACGAGCGCATACCATTCGCTCGATTCTGCTGGAAGTCGAACGGCT
ACACAGTCATTTGCTCAACCTTGGCCTCTCCTGCCATTTTGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGC
GGGAAAAGTCGATGACGATGGCGGAATTGCTGACCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGC
CGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGA
GATGCTGCTTGCTATGCCGAATATGGAACAACGCACTCAGGGCATTGGCATTCTCGACCGACAAATCGCCCGTGATTATA
GCCCTGTAGGGCCGCTGATCCGCGGCAGTGGTTTTGCCCGTGATTTGCGCTTTGATCACCCCTACGCCGACTACGGCAAT
ATTCCAAAAACACTGTTTACCTTCACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTC
GCTGGCAATGCTGGAATTTGCCCTCGACAACATGCCGGATACCCCGCTGCTGACCGAAGGCTTTAGCTATAAACCTCACG
CATTCGCGCTGGGCTTTGTTGAAGCGCCACGCGGTGAAGACGTGCACTGGAGCATGCTCGGTGATAACCAAAAATTGTTC
CGCTGGCGCTGTCGTGCCGCCACCTACGCCAACTGGCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGC
ACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGT
CAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAA

Upstream 100 bases:

>100_bases
ACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTG
GCGAAGCGATTCTCGCCGCC

Downstream 100 bases:

>100_bases
GGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGA
GGTCAGCCCAGGCTTTCGCG

Product: hydrogenase 4, subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKTQFPGAVLDEERQTPEQVTITVKINLLPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKAL
VDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYAFINE
GNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTN
SVENALGIEVSQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGGVR
RDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGN
IPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLF
RWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK

Sequences:

>Translated_555_residues
MKTQFPGAVLDEERQTPEQVTITVKINLLPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKAL
VDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYAFINE
GNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTN
SVENALGIEVSQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGGVR
RDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGN
IPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLF
RWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK
>Mature_555_residues
MKTQFPGAVLDEERQTPEQVTITVKINLLPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKAL
VDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYAFINE
GNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTN
SVENALGIEVSQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGGVR
RDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGN
IPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLF
RWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=381, Percent_Identity=26.246719160105, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI260898743, Length=373, Percent_Identity=26.8096514745308, Blast_Score=107, Evalue=4e-23,
Organism=Escherichia coli, GI1788832, Length=555, Percent_Identity=96.036036036036, Blast_Score=1097, Evalue=0.0,
Organism=Escherichia coli, GI1789076, Length=556, Percent_Identity=72.4820143884892, Blast_Score=876, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=493, Percent_Identity=27.5862068965517, Blast_Score=160, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17555284, Length=392, Percent_Identity=25.765306122449, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17568379, Length=392, Percent_Identity=25.2551020408163, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI221459469, Length=386, Percent_Identity=26.4248704663212, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24638644, Length=380, Percent_Identity=27.6315789473684, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 63451; Mature: 63451

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQFPGAVLDEERQTPEQVTITVKINLLPDVVHYLYYQHDGWLPVLFGNDERTLNGHYA
CCCCCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHEECCCCEEEEECCCCCEECCCEE
VYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQR
EEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCCCC
RLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEP
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCEEECCCCC
GHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTN
CEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHC
SVENALGIEVSQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTM
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHH
AELLTGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQ
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHC
GIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVR
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
VKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCHHHH
RWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYK
HEEHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCHHCCCCCEEEEEEHHHCCCCCCHH
EIERYGIDRNRSPLK
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MKTQFPGAVLDEERQTPEQVTITVKINLLPDVVHYLYYQHDGWLPVLFGNDERTLNGHYA
CCCCCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHEECCCCEEEEECCCCCEECCCEE
VYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQR
EEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCCCC
RLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEP
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCEEECCCCC
GHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTN
CEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHC
SVENALGIEVSQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTM
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHH
AELLTGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQ
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHC
GIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVR
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
VKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCHHHH
RWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYK
HEEHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCHHCCCCCEEEEEEHHHCCCCCCHH
EIERYGIDRNRSPLK
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]