Click here to switch to the map view.

The map label for this gene is hyfF

Identifier: 218696113

GI number: 218696113

Start: 2830738

End: 2832318

Strand: Direct

Name: hyfF

Synonym: EC55989_2771

Alternate gene names: 218696113

Gene position: 2830738-2832318 (Clockwise)

Preceding gene: 218696112

Following gene: 218696114

Centisome position: 54.91

GC content: 54.21

Gene sequence:

>1581_bases
ATGAGTTATTCTGTGATGTTCGCTTTACTCCTGCTCACGCCGCTGCTTTTTTCGCTGCTCTGTTTTGCCTGCCGGAAACG
GGGGCTCTCTGCGACTTGTACGGTGACTGTATTACATAGCTTAGGGATCACACTGCTGCTGATTCTGGCACTCTGGGTGG
TCCAAACTGCCGCTGATGCAGGAGAAATATTCGCTGCGGGACTGTGGCTTCATATTGATGGTCTGGGCGGTTTGTTCCTC
GCCATTCTTGGTGTGATTGGCTTTCTCACCGGTGTTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCT
TTCCCCCGTTACGCTGTGCGATTACTACGGTTTCTTCCATCTGTTTTTGTTTACCATGCTGCTGGTTGTTACCAGCAATA
ACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGTTCA
TCGCTGGAAGCAGCATGGAAGTACATCATTATTTGTACTGTTGGTGTCGCTTTTGGTCTGTTCGGTACCGTGCTGGTATA
CGCCAACGTCGCCAGCGTTATGCCGCAGGCAGAAATGGCGATATTCTGGAGCGAGGTTCTTAAGCAATCGTCCTTGCTTG
ACCCAACATTAATGCTGTTGGCCTTTGTGTTTGTGCTAATTGGCTTTGGCACCAAAACCGGGCTATTTCCCATGCACGCC
TGGCTGCCGGATGCTCACAGTGAAGCACCGAGTCCGGTCAGCGCCCTGCTCTCCGCCGTATTGCTGAACTGCGCGCTGTT
GGTGCTAATTCGCTATTACATCATTATCTGCCAGGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCA
TGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAG
AACATGGGGCTGGTCGCGGTGGCGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACACCTTAAACCA
CAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCT
GTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTC
AACATTTTTCTTAGCGAATTTATGACCGTTACCGCAGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATT
GCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACC
GGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCA
CAACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGACCTGCCTGCACAACGTAGCAC
CTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAA

Upstream 100 bases:

>100_bases
ATCTTCGCCGTCATTGTGATGGTGTTACTGGCAAGAAAAATATGGCGTACCCACGGCACGCTGGACGTGAACAACTTGAC
CGCGCTGAAGGGATAATGAG

Downstream 100 bases:

>100_bases
TTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGAC
AAACGCCTGAACAGGTCACC

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFL
AILGVIGFLTGVYSIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVE
NMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPF
NIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Sequences:

>Translated_526_residues
MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFL
AILGVIGFLTGVYSIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVE
NMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPF
NIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
>Mature_525_residues
SYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLA
ILGVIGFLTGVYSIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSS
LEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAW
LPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVEN
MGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFN
IFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQ
PVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=416, Percent_Identity=27.4038461538462, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI251831116, Length=283, Percent_Identity=25.7950530035336, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788831, Length=526, Percent_Identity=98.4790874524715, Blast_Score=1027, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=357, Percent_Identity=30.5322128851541, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1788829, Length=433, Percent_Identity=29.3302540415704, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1788613, Length=433, Percent_Identity=27.9445727482679, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788614, Length=439, Percent_Identity=28.0182232346241, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI2367154, Length=194, Percent_Identity=36.0824742268041, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693160, Length=205, Percent_Identity=31.7073170731707, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 56532; Mature: 56401

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
GEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEVAHGELSPVTLCDYYGFFH
HHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGL
HHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHH
FGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLL
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
IIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
GEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEVAHGELSPVTLCDYYGFFH
HHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGL
HHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHH
FGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLL
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
IIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]