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The map label for this gene is fruB

Identifier: 218695783

GI number: 218695783

Start: 2477765

End: 2478895

Strand: Reverse

Name: fruB

Synonym: EC55989_2422

Alternate gene names: 218695783

Gene position: 2478895-2477765 (Counterclockwise)

Preceding gene: 218695785

Following gene: 218695782

Centisome position: 48.09

GC content: 55.79

Gene sequence:

>1131_bases
ATGTTCCAGTTATCCGTACAGGACATCCATCCGGGCGAAAAGGCCGGAGACAAAGAAGAGGCGATTCGCCAGGTCGCTGC
GGCGCTGGTGCAGGCCGGTAATGTAGCAGAAGGCTACGTCAATGGCATGCTGGCGCGCGAACAGCAAACCTCAACGTTCC
TCGGCAATGGTATTGCTATTCCACACGGCACTACCGACACCCGCGATCAGGTGCTGAAAACCGGCGTTCAGGTATTTCAG
TTCCCGGAAGGCGTCACCTGGGGTGACGGTCAGGTAGCGTACGTGGCAATCGGTATTGCTGCCAGCTCGGATGAACATCT
GGGCCTGCTACGCCAGCTGACCCGCGTACTGAGCGATGATTCCGTTGCTGAACAACTGAAGTCAGCAACAACAGCAGAAG
AACTTCGCGCATTGCTGATGGGCGAAAAGCAGAGTGAGCAGCTGAAGCTCGACAACGAAATGCTGACGCTGGATATCGCC
GCCAGCGATCTGCTGACTCTTCAGGCGCTGAACGCTGCGCGTCTGAAAGAGGCGAGGGCAGTTGACGCCACTTTCGTCAC
CAAAGCCATCAATGAACAACCGCTGAACCTCGGACAGGGTATCTGGCTGAGCGATAGCGCCGAAGGCAATCTGCGTAGCG
CGATTGCGGTAAGCCGTGCGGCAAATGCTTTTGATGTGGACGGCGAAACGGCAGCCATGCTGGTGAGTGTGGCGATGAAT
GACGATCAGCCCATCGCGGTTCTTAAGCGTCTCGCTGATTTATTGCTCGACAATAAAGCTGACCGCTTGCTGAAAGCAGA
TGCGGCAACGTTGCTGGCGCTGCTGACCAGCGATGATGCGCCGACCGACGACGTGTTAAGCGCGGAGTTTGTGGTGCGCA
ATGAACACGGCCTGCATGCTCGTCCAGGTACCATGCTGGTCAATACCATTAAACAATTTAACAGTGATATTACCGTGACA
AACCTTGATGGTACCGGCAAACCGGCAAACGGACGTAGTCTGATGAAAGTTGTGGCACTTGGCGTTAAGAAAGGTCATCG
CCTACGCTTTACCGCCCAGGGTGCAGATGCTGAACAGGCGCTGAAAGCCATTGGCGACGCTATCGCTGCTGGTCTTGGGG
AGGGCGCATAA

Upstream 100 bases:

>100_bases
TGAAACGTTTCAGCGCGATCTTGTCTTTAACCCTAAGCCAGGTTGGCGCTTTTTTTCTCATAGAGGCTGAATCGTTTCAA
TTCAGCAAGAGAGGAGAACT

Downstream 100 bases:

>100_bases
TGAGCAGACGTGTTGCTACTATCACCCTTAATCCGGCTTATGACCTTGTTGGTTTCTGCCCGGAAATTGAACGCGGCGAA
GTGAACCTGGTGAAAACCAC

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPEGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTRVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMN
DDQPIAVLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVT
NLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA

Sequences:

>Translated_376_residues
MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPEGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTRVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMN
DDQPIAVLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVT
NLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA
>Mature_376_residues
MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPEGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTRVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMN
DDQPIAVLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVT
NLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=376, Percent_Identity=99.2021276595745, Blast_Score=741, Evalue=0.0,
Organism=Escherichia coli, GI1790027, Length=145, Percent_Identity=46.2068965517241, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 39738; Mature: 39738

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAI
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEC
PHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTRVLSDD
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCC
SVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIAASDLLTLQALNAARLKEARA
HHHHHHHHCCCHHHHHHHHHCCCCCHHEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHH
VDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMN
HHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHEEEEEEECC
DDQPIAVLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCC
RPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGADAEQA
CCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHH
LKAIGDAIAAGLGEGA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAI
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEC
PHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTRVLSDD
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCC
SVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIAASDLLTLQALNAARLKEARA
HHHHHHHHCCCHHHHHHHHHCCCCCHHEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHH
VDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMN
HHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHEEEEEEECC
DDQPIAVLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCC
RPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGADAEQA
CCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHH
LKAIGDAIAAGLGEGA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]