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The map label for this gene is fruK

Identifier: 218695782

GI number: 218695782

Start: 2476827

End: 2477765

Strand: Reverse

Name: fruK

Synonym: EC55989_2421

Alternate gene names: 218695782

Gene position: 2477765-2476827 (Counterclockwise)

Preceding gene: 218695783

Following gene: 218695781

Centisome position: 48.07

GC content: 53.14

Gene sequence:

>939_bases
ATGAGCAGACGTGTTGCTACTATCACCCTTAATCCGGCTTATGACCTTGTTGGTTTCTGCCCGGAAATTGAACGCGGCGA
AGTGAACCTGGTGAAAACCACCGGTCTGCATGCGGCGGGTAAAGGCATCAACGTGGCCAAAGTATTAAAAGACCTGGGAA
TTGATGTCACCGTTGGCGGCTTCCTGGGTAAAGACAATCAGGATGGTTTTCAACAACTGTTCAGCGAGCTGGGCATTGCC
AACCGTTTCCAGGTTGTACAGGGGCGCACCCGAATTAACGTTAAGCTGACGGAAAAAGACGGCGAAGTGACCGACTTCAA
CTTCTCGGGTTTTGAAGTCACCCCCGCCGACTGGGAACGCTTTGTGACTGATTCTCTGAGCTGGCTCGGTCAGTTCGATA
TGGTCTGTGTCAGCGGAAGCTTACCGTCAGGCGTCAGCCCGGAAGCGTTCACCGACTGGATGACTCGCCTGCGTAGTCAG
TGTCCTTGCATTATCTTTGATAGTAGCCGTGAAGCGTTAGTAGCAGGTTTGAAAGCGGCACCGTGGCTGGTGAAACCTAA
CCGCCGCGAGTTGGAAATCTGGGCAGGCCGTAAACTGCCTGAAATGAAAGATGTGATTGAAGCTGCACATGCGCTACGTG
AACAAGGTATTGCGCATGTTGTTATTTCACTGGGTGCCGAAGGCGCGCTTTGGGTTAATGCCTCCGGCGAATGGATCGCC
AAACCACCGTCAGTCGATGTCGTAAGCACCGTTGGCGCAGGGGATTCTATGGTTGGTGGCCTGATTTATGGCTTGCTGAT
GCGTGAATCCAGTGAACACACACTGCGTCTGGCGACAGCTGTTGCCGCCCTGGCGGTAAGTCAAAGCAATGTGGGTATTA
CCGATCGTCCGCAGTTGGCCGCAATGATGGCGCGCGTCGACTTACAACCTTTTAACTGA

Upstream 100 bases:

>100_bases
AAGGTCATCGCCTACGCTTTACCGCCCAGGGTGCAGATGCTGAACAGGCGCTGAAAGCCATTGGCGACGCTATCGCTGCT
GGTCTTGGGGAGGGCGCATA

Downstream 100 bases:

>100_bases
CAGCAGGAGAGGCATAATGAAAACGCTGCTGATTATTGACGCTAATCTCGGTCAGGCACGCGCCTATATGGCGAAGACCC
TGCTGGGCGCGGCGGCGCGA

Product: 1-phosphofructokinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGINVAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIA
NRFQVVQGRTRINVKLTEKDGEVTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIAHVVISLGAEGALWVNASGEWIA
KPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN

Sequences:

>Translated_312_residues
MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGINVAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIA
NRFQVVQGRTRINVKLTEKDGEVTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIAHVVISLGAEGALWVNASGEWIA
KPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN
>Mature_311_residues
SRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGINVAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIAN
RFQVVQGRTRINVKLTEKDGEVTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQC
PCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIAHVVISLGAEGALWVNASGEWIAK
PPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN

Specific function: Unknown

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family

Homologues:

Organism=Escherichia coli, GI1788493, Length=312, Percent_Identity=100, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1788017, Length=293, Percent_Identity=33.4470989761092, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): K1PF_ECO57 (P0AEX1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91011
- PIR:   F85855
- RefSeq:   NP_288751.1
- RefSeq:   NP_311087.1
- ProteinModelPortal:   P0AEX1
- SMR:   P0AEX1
- EnsemblBacteria:   EBESCT00000028859
- EnsemblBacteria:   EBESCT00000059676
- GeneID:   916764
- GeneID:   959326
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3426
- KEGG:   ecs:ECs3060
- GeneTree:   EBGT00070000031691
- HOGENOM:   HBG670529
- OMA:   GIDNTAT
- ProtClustDB:   PRK09513
- BioCyc:   ECOL83334:ECS3060-MONOMER
- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583
- TIGRFAMs:   TIGR03168
- TIGRFAMs:   TIGR03828

Pfam domain/function: PF00294 PfkB

EC number: =2.7.1.56

Molecular weight: Translated: 33756; Mature: 33625

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2; PS00464 RIBOSOMAL_L22

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGINVAKVLKDLGIDVTVGG
CCCEEEEEEECCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCEEEECC
FLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGEVTDFNFSGFEVTPADWER
CCCCCCHHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCCCCEEEECCCCEEECHHHHHH
FVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQCPCIIFDSSREALVAGLKAA
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCCC
PWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIAHVVISLGAEGALWVNASGEWIA
CEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCEEE
KPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGITDRPQLA
CCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
AMMARVDLQPFN
HHHHHHCCCCCC
>Mature Secondary Structure 
SRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGINVAKVLKDLGIDVTVGG
CCEEEEEEECCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCEEEECC
FLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGEVTDFNFSGFEVTPADWER
CCCCCCHHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCCCCEEEECCCCEEECHHHHHH
FVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQCPCIIFDSSREALVAGLKAA
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCCC
PWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIAHVVISLGAEGALWVNASGEWIA
CEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCEEE
KPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGITDRPQLA
CCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
AMMARVDLQPFN
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796