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The map label for this gene is insI

Identifier: 218695568

GI number: 218695568

Start: 2221472

End: 2222623

Strand: Reverse

Name: insI

Synonym: EC55989_2188

Alternate gene names: 218695568

Gene position: 2222623-2221472 (Counterclockwise)

Preceding gene: 218695570

Following gene: 218695567

Centisome position: 43.12

GC content: 45.83

Gene sequence:

>1152_bases
ATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGC
GAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGC
GGGCTGTAGCTCACCTGACACTGTCTGAGCGCGAGGAGATACGAGCTGGTTTGTCAGCCAAAATGAGCATTCGTGCGATA
GCTACTGCGCTGAATCGCAGTCCTTCGACGATCTCACGTGAAGTTCAGCGTAATCGGGGCAGACGCTATTACAAAGCTGT
TGATGCTAATAACCGAGCCAACAGAATGGCGAAAAGGCCAAAACCGTGCTTACTGGATCAAAATTTACCATTGCGAAAGC
TTGTTCTGGAAAAGCTGGAGATGAAATGGTCTCCAGAGCAAATATCAGGATGGTTAAGGCGAACAAAACCACGTCAAAAA
ACGCTGCGAATATCACCTGAGACAATTTATAAAACGCTGTACTTTCGTAGCCGTGAAGCGCTACACCACCTGAATATACA
GCATCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGA
ACGGAACACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCA
GGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGATCATCCTTAGACTCAGGGGCAAAGA
TTCTGTCTTAGTAAATCAGGCTCTTACCGACAAATTCCTGAGTTTACCGTCAGAACTCAGAAAATCACTGACATGGGACA
GAGGAATGGAACTGGCCAGACATCTAGAATTTACTGTCAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCT
TGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATAC
TCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGA
TAATTGAAAGGGGTGTTGCATTGACAGATTGA

Upstream 100 bases:

>100_bases
CGCGTATTTTTCAGCGACTCCTGTGCAAAATACGCTGTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATG
GGGTTGCGGAGGTTTTTTGA

Downstream 100 bases:

>100_bases
ATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATT
CCAGTTCAGCCACCGTGCGG

Product: IS30 transposase; KpLE2 phage-like element

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAI
ATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
GTKNSHIATLVDRKSRYTIILRLRGKDSVLVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSP
WQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD

Sequences:

>Translated_383_residues
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAI
ATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
GTKNSHIATLVDRKSRYTIILRLRGKDSVLVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSP
WQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD
>Mature_383_residues
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAI
ATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
GTKNSHIATLVDRKSRYTIILRLRGKDSVLVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSP
WQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790736, Length=383, Percent_Identity=99.7389033942559, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI1787671, Length=383, Percent_Identity=99.7389033942559, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI1786450, Length=383, Percent_Identity=99.4778067885117, Blast_Score=784, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 44308; Mature: 44308

Theoretical pI: Translated: 11.48; Mature: 11.48

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLT
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHEE
LSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRP
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCC
KPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSREA
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHEEECCHHHHHHHHHHHHHHH
LHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
HHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCHHHCCCCCCCCCCC
GTKNSHIATLVDRKSRYTIILRLRGKDSVLVNQALTDKFLSLPSELRKSLTWDRGMELAR
CCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHH
HLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNN
HHHHEEECCEEEEEECCCCHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
RPRKTLKFKTPKEIIERGVALTD
CCCCCEECCCHHHHHHCCCCCCC
>Mature Secondary Structure
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLT
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHEE
LSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRP
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCC
KPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSREA
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHEEECCHHHHHHHHHHHHHHH
LHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
HHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCHHHCCCCCCCCCCC
GTKNSHIATLVDRKSRYTIILRLRGKDSVLVNQALTDKFLSLPSELRKSLTWDRGMELAR
CCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHH
HLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNN
HHHHEEECCEEEEEECCCCHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
RPRKTLKFKTPKEIIERGVALTD
CCCCCEECCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]