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The map label for this gene is fliJ

Identifier: 218695547

GI number: 218695547

Start: 2203985

End: 2204428

Strand: Direct

Name: fliJ

Synonym: EC55989_2162

Alternate gene names: 218695547

Gene position: 2203985-2204428 (Clockwise)

Preceding gene: 218695546

Following gene: 218695548

Centisome position: 42.76

GC content: 52.03

Gene sequence:

>444_bases
ATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGA
AATGCGTCGCGGATGTCAGCAGGCGGAAGAACAGCTCAAAATGCTGATCGATTATCAGAATGAATATCGCAATAACCTCA
ATAGCGATATGAGTGCCGGGATGACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGACGTTGGAAAAAGCCATT
ACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGCTGGCGAGAAAAGAAACAACG
TTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGA
TGGATGAGTTCGCCCAGCGCGCCGCCATGAGGAAACCTGAATGA

Upstream 100 bases:

>100_bases
TTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCAT
AACCCAGGAGATAACGGCAG

Downstream 100 bases:

>100_bases
TTCGCTTAGCGCCCTTGATTACCGCCGACGTTGACACCACCACATTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGAT
TTTCTCGCGTTGTTGAGCGA

Product: flagellar biosynthesis chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAI
TQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE

Sequences:

>Translated_147_residues
MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAI
TQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE
>Mature_146_residues
AEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAIT
QHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE

Specific function: The fliJ protein is a flagellar protein that affects chemotactic events. Mutations in fliJ results in failure to respond to chemotactic stimuli [H]

COG id: COG2882

COG function: function code NUO; Flagellar biosynthesis chaperone

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliJ family [H]

Homologues:

Organism=Escherichia coli, GI1788252, Length=147, Percent_Identity=99.3197278911565, Blast_Score=296, Evalue=4e-82,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018006
- InterPro:   IPR012823 [H]

Pfam domain/function: PF02050 FliJ [H]

EC number: NA

Molecular weight: Translated: 17325; Mature: 17194

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
MTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQST
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALLAENRLDQKKMDEFAQRAAMRKPE
HHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
MTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQST
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALLAENRLDQKKMDEFAQRAAMRKPE
HHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8631687 [H]