Click here to switch to the map view.

The map label for this gene is mipA

Identifier: 218695341

GI number: 218695341

Start: 2014159

End: 2014905

Strand: Reverse

Name: mipA

Synonym: EC55989_1951

Alternate gene names: 218695341

Gene position: 2014905-2014159 (Counterclockwise)

Preceding gene: 218695347

Following gene: 218695340

Centisome position: 39.09

GC content: 52.07

Gene sequence:

>747_bases
GTGACCAAACTCAAACTTCTGGCACTTGGAGTGCTTATCGCAACGTCTGCAGGCGTAGCGCACGCTGAAGGTAAATTTTC
CCTGGGCGCAGGCGTAGGTGTCGTTGAACACCCATATAAAGATTACGATACCGATGTTTACCCAGTACCGGTAATCAACT
ATGAAGGCGATAACTTCTGGTTCCGTGGCTTAGGTGGTGGTTACTACCTGTGGAATGACGCAACGGATAAACTTTCAATT
ACCGCTTACTGGTCGCCGCTTTACTTCAAAGCGAAAGACAGTGGCGATCACCAAATGCGTCACCTGGATGACCGTAAGAG
CACCATGATGGCTGGTCTGTCTTATGCTCACTTTACCCAGTACGGTTACCTGCGTACCACCCTGGCTGGCGATACCCTGG
ATAACAGCAACGGCATTGTCTGGGATATGGCCTGGTTGTATCGTTACACCAACGGTGGCCTGACCGTGACTCCGGGTATT
GGTGTGCAGTGGAACAGCGAAAACCAGAACGAATACTATTATGGCGTATCGCGCAAAGAGTCCGCTCGCAGCGGTCTGCG
TGGCTATAACCCGAACGACAGCTGGAGCCCTTACCTGGAGCTGAGCGCCAGCTACAACTTCCTCGGCGACTGGAGTGTTT
ACGGTACCGCGCGCTATACCCGTCTGTCTGATGAAGTTACTGACAGCCCGATGGTGGATAAATCCTGGACTGGCCTGATT
TCTACCGGGATCACCTACAAATTCTGA

Upstream 100 bases:

>100_bases
CAGTTACTTTAAAAAATGAAAACGTAAAAAGGTTGGGTTTCGATGTATTGACGGGTAAACTTTGTCGCCCGCTAAACATT
TGTTTAAGGAATGATTAATT

Downstream 100 bases:

>100_bases
TAATGCATCTTTCCAGGGCATTTTGTGCATAAAAACAGGGCGTCACGCCCTGTTTTGCATTACGGTGGTGCAATACCTGG
GAGGAAACAATGCAACAAAA

Product: scaffolding protein for murein synthesizing machinery

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDTDVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSI
TAYWSPLYFKAKDSGDHQMRHLDDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTARYTRLSDEVTDSPMVDKSWTGLI
STGITYKF

Sequences:

>Translated_248_residues
MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDTDVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSI
TAYWSPLYFKAKDSGDHQMRHLDDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTARYTRLSDEVTDSPMVDKSWTGLI
STGITYKF
>Mature_247_residues
TKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDTDVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSIT
AYWSPLYFKAKDSGDHQMRHLDDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGIG
VQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTARYTRLSDEVTDSPMVDKSWTGLIS
TGITYKF

Specific function: May serve as a scaffold protein required for the formation of a complex with mrcB/ponB and mltA, this complex could play a role in enlargement and septation of the murein sacculus

COG id: COG3713

COG function: function code M; Outer membrane protein V

Gene ontology:

Cell location: Cell outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mipA/ompV family

Homologues:

Organism=Escherichia coli, GI1788082, Length=248, Percent_Identity=100, Blast_Score=505, Evalue=1e-145,
Organism=Escherichia coli, GI1790010, Length=245, Percent_Identity=37.5510204081633, Blast_Score=184, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIPA_ECOLI (P0A908)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   F64938
- RefSeq:   AP_002401.1
- RefSeq:   NP_416296.1
- ProteinModelPortal:   P0A908
- STRING:   P0A908
- PRIDE:   P0A908
- EnsemblBacteria:   EBESCT00000000770
- EnsemblBacteria:   EBESCT00000014625
- GeneID:   946301
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1771
- KEGG:   eco:b1782
- EchoBASE:   EB3265
- EcoGene:   EG13492
- eggNOG:   COG3713
- GeneTree:   EBGT00050000009458
- HOGENOM:   HBG752379
- OMA:   FIGAAYR
- ProtClustDB:   CLSK880199
- BioCyc:   EcoCyc:G6968-MONOMER
- Genevestigator:   P0A908
- InterPro:   IPR010583

Pfam domain/function: PF06629 MipA

EC number: NA

Molecular weight: Translated: 27831; Mature: 27700

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDTDVYPVPVINYEGDNFW
CCCCHHHHHHHHHHCCCCCEECCCCEEECCCCCEECCCCCCCCCCEEEEEEEEECCCCEE
FRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHLDDRKSTMMAGLSYAHFTQ
EEECCCCEEEECCCCCEEEEEEEECEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHH
YGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGIGVQWNSENQNEYYYGVSRKE
CCEEEEEECCCCCCCCCCEEEEEEEEEEEECCCEEECCCCCEEECCCCCCEEEEECCCCH
SARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTARYTRLSDEVTDSPMVDKSWTGLI
HHHHCCCCCCCCCCCCCEEEEEECCCEEECEEEECCEEEEEECCCCCCCCCCCCCCCCEE
STGITYKF
ECCEEECC
>Mature Secondary Structure 
TKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDTDVYPVPVINYEGDNFW
CCCHHHHHHHHHHCCCCCEECCCCEEECCCCCEECCCCCCCCCCEEEEEEEEECCCCEE
FRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHLDDRKSTMMAGLSYAHFTQ
EEECCCCEEEECCCCCEEEEEEEECEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHH
YGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGIGVQWNSENQNEYYYGVSRKE
CCEEEEEECCCCCCCCCCEEEEEEEEEEEECCCEEECCCCCEEECCCCCCEEEEECCCCH
SARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTARYTRLSDEVTDSPMVDKSWTGLI
HHHHCCCCCCCCCCCCCEEEEEECCCEEECEEEECCEEEEEECCCCCCCCCCCCCCCCEE
STGITYKF
ECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 10037771; 10806384