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The map label for this gene is yaeL

Identifier: 218693641

GI number: 218693641

Start: 195811

End: 197163

Strand: Direct

Name: yaeL

Synonym: EC55989_0170

Alternate gene names: 218693641

Gene position: 195811-197163 (Clockwise)

Preceding gene: 218693640

Following gene: 218693642

Centisome position: 3.8

GC content: 50.78

Gene sequence:

>1353_bases
ATGCTGAGTTTTCTCTGGGATTTGGCTTCGTTCATCGTTGCACTGGGTGTACTTATCACCGTGCATGAATTTGGTCATTT
CTGGGTTGCCCGGCGTTGTAGTGTTCGCGTTGAGCGTTTCTCAATAGGGTTTGGTAAGGCGCTCTGGCGGCGAACTGATA
AGCTCGGCACCGAATATGTTATCGCCCTGATCCCGCTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTC
GTTCCGGAACTCCGCCACCATGCCTTCAATAATAAATCTGTTGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGC
AAACTTCATTTTTGCTATCTTAGCCTACTGGCTGGTTTTTATTATTGGTGTGCCTGGCGTACGTCCGGTGGTTGGTGAAA
TAGCAGCCAATTCGATAGCTGCGGAAGCACAAATTGCACCAGGTACGGAACTAAAAGCCGTAGATGGTATCGAAACGCCT
GATTGGGATGCCGTGCGTTTGCAGTTGGTCGATAAAATTGGCGATGAAAGCACCACCATTACAGTAGCGCCATTTGGCAG
CGACCAACGGCGGGATGTAAAGCTCGATTTACGTCACTGGGCGTTTGAGCCTGATAAAGAAGATCCGGTATCTTCGCTGG
GGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGAAAATGTGCAGCCAAACTCGGCGGCAAGCAAGGCAGGTTTG
CAAGCAGGCGACAGGATCGTTAAAGTCGATGGTCAGCCCTTAACGCAGTGGGTGACCTTTGTGATGCTTGTCCGGGATAA
CCCGGGTAAATCCTTAGCGTTAGAAATCGAAAGGCAGGGGAGTCCCTTGTCTTTGACATTAATCCCGGAGAGTAAACCGG
GTAATGGTAAAGCGATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAG
TATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGTATGCTGGGAAA
ATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAAC
TCGGGGTAGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTA
CTAGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTG
TTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGA

Upstream 100 bases:

>100_bases
ACGGTGGTATTTTAGATCGTATTGATAGCCTGACGGCTGCGGTACCGGTCTTTGCTTGCTTGTTGTTACTGGTATTCAGG
ACGCTTTAACGGAAGGTAAT

Downstream 100 bases:

>100_bases
GAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACC
GTATACGGTGCTGAAGGGTT

Product: zinc metallopeptidase RseP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLSFLWDLASFIVALGVLITVHEFGHFWVARRCSVRVERFSIGFGKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPV
VPELRHHAFNNKSVGQRAAIIAAGPVANFIFAILAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGL
QAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQ
YGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL

Sequences:

>Translated_450_residues
MLSFLWDLASFIVALGVLITVHEFGHFWVARRCSVRVERFSIGFGKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPV
VPELRHHAFNNKSVGQRAAIIAAGPVANFIFAILAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGL
QAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQ
YGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
>Mature_450_residues
MLSFLWDLASFIVALGVLITVHEFGHFWVARRCSVRVERFSIGFGKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPV
VPELRHHAFNNKSVGQRAAIIAAGPVANFIFAILAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGL
QAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQ
YGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL

Specific function: Cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of rseA. Acts on degS-cleaved rseA to release the cytoplasmic domain of rseA. This provides the cell with sigma E (rpoE) activity through the proteolysis of rseA. In vitr

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=450, Percent_Identity=99.5555555555556, Blast_Score=899, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 49068; Mature: 49068

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50106 PDZ ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSFLWDLASFIVALGVLITVHEFGHFWVARRCSVRVERFSIGFGKALWRRTDKLGTEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHCCCEEE
IALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIAAGPVANFIFAILAYWLVF
EEEECCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
IIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETPDWDAVRLQLVDKIGDESTTI
HHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCEE
TVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGL
EEEECCCCCCCCEEEEHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHCCCCCCHHHHCCC
QAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIG
CCCCEEEEECCCCHHHHHHEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEE
FVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSG
EEECCCEEEECCCHHHHHHHCCCCHHEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
PISIAKGAGMTAELGVVYYLPFLALISVNLGIINLFPLPVLDGGHLLFLAIEKIKGGPVS
CEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHCCCCHH
ERVQDFCYRIGSILLVLLMGLALFNDFSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSFLWDLASFIVALGVLITVHEFGHFWVARRCSVRVERFSIGFGKALWRRTDKLGTEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHCCCEEE
IALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIAAGPVANFIFAILAYWLVF
EEEECCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
IIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETPDWDAVRLQLVDKIGDESTTI
HHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCEE
TVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGL
EEEECCCCCCCCEEEEHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHCCCCCCHHHHCCC
QAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIG
CCCCEEEEECCCCHHHHHHEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEE
FVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSG
EEECCCEEEECCCHHHHHHHCCCCHHEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
PISIAKGAGMTAELGVVYYLPFLALISVNLGIINLFPLPVLDGGHLLFLAIEKIKGGPVS
CEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHCCCCHH
ERVQDFCYRIGSILLVLLMGLALFNDFSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]