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The map label for this gene is dctB [H]

Identifier: 218692344

GI number: 218692344

Start: 4738634

End: 4740454

Strand: Reverse

Name: dctB [H]

Synonym: ECED1_4779

Alternate gene names: 218692344

Gene position: 4740454-4738634 (Counterclockwise)

Preceding gene: 218692348

Following gene: 218692343

Centisome position: 91.0

GC content: 40.58

Gene sequence:

>1821_bases
ATGAAAATATCGTGGAATTATATATTTAAGAACAAATGGCGATTTCACATTACAAGCATTTCACTTTTTCTTATCATGCT
CGCGGTTTCAATCGCTTTTTTGCACTTGCGTTTTAATACCTTGTCCGGTACCGATAAAATGCGGCTTGAAATGTATAAGT
CCACATTATATTCCACCATCGAGCAATTTTATGTTTTACCCTATATGCTCTCAACAGACCATATCATCCGTCAGGCGGTA
ATTACGCCTGACGATATGACGTCCAGCGAACTCAATCAACGAATTGCACATTTCAATACTCAACTCAAAACCGCAGCAAT
ATTTATTCTGGATACCCAAGGTAAGGCCATCGCTTCTAGCAACTGGCAGGACCCCGGCAGCTATGTAGGGCAAAATTATA
GCTATCGCCCCTATTATAAACACGCCATGTCTGGCTTAAATGGACGCTTTTACGGTATTGGTAGCACTACAAATACACCG
GGATTCTTCCTCTCTACAAGTATAAAAGATAAAGGAAAAATTGTCGGTGTTGTAGTAGTAAAAATAAGTCTTAATGAAAT
TGAAAAAGCATGGTCCGAAGGTCCTGAAAACATTATCGTTAATGATGAACATAGGATTATATTTTTAAGTTCAAAATCGC
CATGGCGAATGAGAACGCTGCAACCGTTACCTGTTCAGGCAAAACAAAAACTTCAATCTACCCGTCAATATAGCCTGGAC
AATCTTTTACCTGCGGATTATTACCCTTGTTTTACCGTTAGCAATTTTACTTTCCTGAAAGATAAAAAAGAACAATTCTG
CTTATTCCCGCAGTATTATACGCAACAAATCGCCATTCCAGAGTTTAACTGGAAAATGACAATTATGGTCCCCTTAGATA
ACCTGTACTGGTCATGGGCTATTTCGTTAGTCATTACACTAATTATTTACCTGCTGTTTTTGTTATTTATTAAATACTGG
AGAATGCGATCTCATGCACAACAATTATTAACACTGGCGAATGAAACATTAGAAAAACAGGTTAAAGAGCGTACATCTGT
CCTGGAATTGATCAATCAAAAATTAATACAGGAGATAAAAGAGCGCAGTCAGGCTGAACAAGTATTACAAATTACGCGTA
GTGAACTGGCAGAGTCCAGCAAACTGGCGGCGCTTGGACAGATGGCAACCGAAATTGCCCATGAACAAAATCAACCGTTA
GCCGCCATTCACGCACTTACTGATAACGCGCGTACTATGCTAAAAAAAGAGATGTATCCGCAGGTTGAACAGAATCTGAA
ACACATTATTTCAGTGATTGAGCGGATGACGCAGCTCATTTCCGAACTTAAAGCATTTGCCTCGCGCCATCGCGTACCTA
AAGGTTCTGCCGATGTCATCAAAGTGATGTATAGCGCCGTGGCGTTACTTAATCACAGCATGGAGAAAAATAACATTGAG
CGACGAATAAAAGCCCCATCCATGCCGTTATTTGTCAATTGCGATGAGCTCGGTCTTGAACAGATATTCAGTAATTTAAT
TAGCAACGCCTTAGATTCTATGGAAGGTAGCTCTTACAAACGACTGGATATCGCCATTCGCCAGGCAAATAACAAAGTTA
TTATTACCATTAAAGACAGCGGTGGCGGTTTTGCACCTGAAGTTGTCGATCGCATATTTGAACCATTTTTTACCACTAAA
CGTAGAGGAATGGGGTTGGGACTGGCAATAGTCAGCGAAATTGTCCGAAATTCGAACGGCGCACTCCACGCCAGTAATCA
TCCTGAAGGCGGCGCAGTAATGACATTAACCTGGCCTGAATGGGGAGAAGAACATGAATAA

Upstream 100 bases:

>100_bases
TTCTGCTTACACATTCACACAATGGCACTCTTTTGGTTCTCGCAAACATTGTTCAGGGAATATACTGATGATAAATATAA
ATGCATTCAATGGATGATGA

Downstream 100 bases:

>100_bases
TAAATCCAGTGAAATTACTATAGTTTATATTGAAGATAGTGACGATGTACGTTTTGCCTGTGAACAAACGCTCACGCTGG
CTGGTTATCGTGTTATTTCC

Product: putative C4-dicarboxylate transport sensor protein (dctB-like)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGTDKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAV
ITPDDMTSSELNQRIAHFNTQLKTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWSEGPENIIVNDEHRIIFLSSKSPWRMRTLQPLPVQAKQKLQSTRQYSLD
NLLPADYYPCFTVSNFTFLKDKKEQFCLFPQYYTQQIAIPEFNWKMTIMVPLDNLYWSWAISLVITLIIYLLFLLFIKYW
RMRSHAQQLLTLANETLEKQVKERTSVLELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEIAHEQNQPL
AAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRHRVPKGSADVIKVMYSAVALLNHSMEKNNIE
RRIKAPSMPLFVNCDELGLEQIFSNLISNALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTK
RRGMGLGLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEEHE

Sequences:

>Translated_606_residues
MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGTDKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAV
ITPDDMTSSELNQRIAHFNTQLKTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWSEGPENIIVNDEHRIIFLSSKSPWRMRTLQPLPVQAKQKLQSTRQYSLD
NLLPADYYPCFTVSNFTFLKDKKEQFCLFPQYYTQQIAIPEFNWKMTIMVPLDNLYWSWAISLVITLIIYLLFLLFIKYW
RMRSHAQQLLTLANETLEKQVKERTSVLELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEIAHEQNQPL
AAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRHRVPKGSADVIKVMYSAVALLNHSMEKNNIE
RRIKAPSMPLFVNCDELGLEQIFSNLISNALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTK
RRGMGLGLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEEHE
>Mature_606_residues
MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGTDKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAV
ITPDDMTSSELNQRIAHFNTQLKTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWSEGPENIIVNDEHRIIFLSSKSPWRMRTLQPLPVQAKQKLQSTRQYSLD
NLLPADYYPCFTVSNFTFLKDKKEQFCLFPQYYTQQIAIPEFNWKMTIMVPLDNLYWSWAISLVITLIIYLLFLLFIKYW
RMRSHAQQLLTLANETLEKQVKERTSVLELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEIAHEQNQPL
AAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRHRVPKGSADVIKVMYSAVALLNHSMEKNNIE
RRIKAPSMPLFVNCDELGLEQIFSNLISNALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTK
RRGMGLGLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEEHE

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=26.8398268398268, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=26.3829787234043, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787894, Length=315, Percent_Identity=26.3492063492064, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI145693157, Length=267, Percent_Identity=24.7191011235955, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081816, Length=292, Percent_Identity=26.3698630136986, Blast_Score=70, Evalue=6e-13,
Organism=Escherichia coli, GI1788393, Length=222, Percent_Identity=27.027027027027, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=219, Percent_Identity=28.7671232876712, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1790346, Length=223, Percent_Identity=24.6636771300448, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 69394; Mature: 69394

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGTDKMRLEMYKSTLYSTI
CEEEEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHH
EQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQLKTAAIFILDTQGKAIASS
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEECCCCEECCC
NWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTPGFFLSTSIKDKGKIVGVVVV
CCCCCHHHHCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCCCEEEEEEE
KISLNEIEKAWSEGPENIIVNDEHRIIFLSSKSPWRMRTLQPLPVQAKQKLQSTRQYSLD
EEEHHHHHHHHHCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NLLPADYYPCFTVSNFTFLKDKKEQFCLFPQYYTQQIAIPEFNWKMTIMVPLDNLYWSWA
CCCCCCCCCCEEECCEEEECCCCHHEEECCHHHHCEEECCCCCEEEEEEEECCHHHHHHH
ISLVITLIIYLLFLLFIKYWRMRSHAQQLLTLANETLEKQVKERTSVLELINQKLIQEIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERSQAEQVLQITRSELAESSKLAALGQMATEIAHEQNQPLAAIHALTDNARTMLKKEMYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCH
QVEQNLKHIISVIERMTQLISELKAFASRHRVPKGSADVIKVMYSAVALLNHSMEKNNIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRIKAPSMPLFVNCDELGLEQIFSNLISNALDSMEGSSYKRLDIAIRQANNKVIITIKDS
HHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCEEEEEEECC
GGGFAPEVVDRIFEPFFTTKRRGMGLGLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPE
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEECCCCCCCCEEEEEECCC
WGEEHE
CCCCCC
>Mature Secondary Structure
MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGTDKMRLEMYKSTLYSTI
CEEEEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHH
EQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQLKTAAIFILDTQGKAIASS
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEECCCCEECCC
NWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTPGFFLSTSIKDKGKIVGVVVV
CCCCCHHHHCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCCCEEEEEEE
KISLNEIEKAWSEGPENIIVNDEHRIIFLSSKSPWRMRTLQPLPVQAKQKLQSTRQYSLD
EEEHHHHHHHHHCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NLLPADYYPCFTVSNFTFLKDKKEQFCLFPQYYTQQIAIPEFNWKMTIMVPLDNLYWSWA
CCCCCCCCCCEEECCEEEECCCCHHEEECCHHHHCEEECCCCCEEEEEEEECCHHHHHHH
ISLVITLIIYLLFLLFIKYWRMRSHAQQLLTLANETLEKQVKERTSVLELINQKLIQEIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERSQAEQVLQITRSELAESSKLAALGQMATEIAHEQNQPLAAIHALTDNARTMLKKEMYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCH
QVEQNLKHIISVIERMTQLISELKAFASRHRVPKGSADVIKVMYSAVALLNHSMEKNNIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRIKAPSMPLFVNCDELGLEQIFSNLISNALDSMEGSSYKRLDIAIRQANNKVIITIKDS
HHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCEEEEEEECC
GGGFAPEVVDRIFEPFFTTKRRGMGLGLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPE
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEECCCCCCCCEEEEEECCC
WGEEHE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]