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The map label for this gene is yfkN [H]

Identifier: 218692226

GI number: 218692226

Start: 4584713

End: 4586257

Strand: Reverse

Name: yfkN [H]

Synonym: ECED1_4645

Alternate gene names: 218692226

Gene position: 4586257-4584713 (Counterclockwise)

Preceding gene: 218692231

Following gene: 218692225

Centisome position: 88.04

GC content: 43.95

Gene sequence:

>1545_bases
GTGGTCAAATTCATGGTTGCCTATTGTGACAGCATCAAACGGCATAGTATTCATAATATCGATAATACCGGAAAACGCGA
CATTGGTGGCTTTGCCAATATATCCACGTTAGTAAAACAAGAGAAAGCCAAAAATAAAGCCACCTTTTATTTTGATGCTG
GAGACTATTTCACCGGGCCATATATCAGTAGCCTGACAAAGGGCGAAGCAATAATTGATATTATGAATACCATGCCTTTT
GACGCAGTATCAATCGGTAATCATGAGTTTGACCATGGCTGGGATAATGCCCTCCGACAATTAAGCAAAGCGAATTTCCC
TGTTTTATTAGGCAATGTTTATCATAAAGAGAGTGAGAAACCCTTCTGGAACAAGCCCTACACCATCCTGGAAAAAGATG
GCATCAAGATTGGTATTGTTGGGCTACACGGCGTATTTGCATTTAATGACACTGTCTCGGAGCTCTCTCTCCAGGGACTC
GATAATGACAATAATAACCGATTTGATAAATCTTCCGCGACCCTCAAGAATCAGGGGATTGAAGCACGTGATGAAGTGAA
GTATTTGCAGCATTATATCGACGAGTTACGTGATAAGGTTGACCTCACCGTGGCGCTGGTTCATGAAGGCGTACCGGCGC
GTCAGTCCAGCATTGGCAATACAGATGTTAGACGTGCGCTGGATAAAGATATTCAAACCGCAAGCAAGGTGAAAGGATTG
GATATTCTGATTACCGGGCATGCCCACGTCGGAACGCCAGAACCAATCAAAGTCGGCAACACCTTAATCCTTTCGACTGA
CAGTGGCGGTATCGATATTGGTAAATTAATCCTTGATGTCAACCCTACAGCTCGTACTCATAAAGTGAAGAGCTTTGAGC
TGAAAACGGTTTATGCCGATGAATGGATACCCGATCCAACCACACAAAAAGTTATCAATGGCTGGAATAAAAAACTGGTG
GATATTGTACGCCAACCAGTCGGTGAATCATCGATTGCATTAACTCGTGCCTACGGTGAGTCATCGCAACTTGGCAACCT
GTTTACCGATGCAATGCTTGTCGCTGCACCGACTGCACAAATTGCGTTGATAAACTCCGGCAGTTTACGTGCTGATATAA
ATGCAGGCACGATTACCTTTGGTGACATTACCAGTACATTCCCCTTCAAGAATGAACTTACTGAAATGGATCTCAGCGGT
AAGGATCTGCGTAACCTACTGGAGCACGGGGCATCGCTTACCAATGGCATCCTGCAAATGTCAAAAGGCGCAGAAATGCG
CTATACCCTGCAGAAACCAGTAGGTCAGCGCATTGTATCTTTTAAAATTAATGGTGAAGAGATCGTTGATACGAATATTT
ACCATGTTGCGACTACGACTTTCCTAGCACTTGGGGGCGATGGTTTTCTGGCATTCAAAGAAGGGAAAAATGTCCAGGTC
CGCGCCGGAAATAACATGTCCAATGTCGTAATCGATTATTTTAAGAAAGGCCACAAGATTACTCCTGCACAGGTAAATGA
AATGCGGGTAGAAGTAAGCAAATAA

Upstream 100 bases:

>100_bases
GCTTATCCCAGAATGATTTACTGCTGTTCTGATAAAAAATATTGCCCTGCACAATAGGGAATTTTGCCTGGCTCAACTGT
AATAATGTGTTGTCCCAGCC

Downstream 100 bases:

>100_bases
CAAAAACTTATAATACCATTCATTCACTGGATGACATTATTTCTCTCTAAATCAACAATATATAAGAGATATATTTATGT
ATATAAAAAAGCACTGGATA

Product: putative 5'-nucleotidase/2',3'-cyclic phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MVKFMVAYCDSIKRHSIHNIDNTGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNTMPF
DAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEKPFWNKPYTILEKDGIKIGIVGLHGVFAFNDTVSELSLQGL
DNDNNNRFDKSSATLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGL
DILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQKVINGWNKKLV
DIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINAGTITFGDITSTFPFKNELTEMDLSG
KDLRNLLEHGASLTNGILQMSKGAEMRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQV
RAGNNMSNVVIDYFKKGHKITPAQVNEMRVEVSK

Sequences:

>Translated_514_residues
MVKFMVAYCDSIKRHSIHNIDNTGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNTMPF
DAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEKPFWNKPYTILEKDGIKIGIVGLHGVFAFNDTVSELSLQGL
DNDNNNRFDKSSATLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGL
DILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQKVINGWNKKLV
DIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINAGTITFGDITSTFPFKNELTEMDLSG
KDLRNLLEHGASLTNGILQMSKGAEMRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQV
RAGNNMSNVVIDYFKKGHKITPAQVNEMRVEVSK
>Mature_514_residues
MVKFMVAYCDSIKRHSIHNIDNTGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNTMPF
DAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEKPFWNKPYTILEKDGIKIGIVGLHGVFAFNDTVSELSLQGL
DNDNNNRFDKSSATLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGL
DILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQKVINGWNKKLV
DIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINAGTITFGDITSTFPFKNELTEMDLSG
KDLRNLLEHGASLTNGILQMSKGAEMRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQV
RAGNNMSNVVIDYFKKGHKITPAQVNEMRVEVSK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=540, Percent_Identity=26.1111111111111, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1790658, Length=582, Percent_Identity=21.6494845360825, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786687, Length=119, Percent_Identity=36.9747899159664, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24652512, Length=476, Percent_Identity=25, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19921980, Length=476, Percent_Identity=25, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI161076508, Length=476, Percent_Identity=25, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24641187, Length=512, Percent_Identity=25.5859375, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI221329836, Length=507, Percent_Identity=24.8520710059172, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922446, Length=542, Percent_Identity=25.4612546125461, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24654424, Length=542, Percent_Identity=25.4612546125461, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28573524, Length=545, Percent_Identity=24.5871559633028, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19922444, Length=545, Percent_Identity=24.2201834862385, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 56591; Mature: 56591

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKFMVAYCDSIKRHSIHNIDNTGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGP
CCEEEEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
YISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEK
HHHHHCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEECCHHHCCCCC
PFWNKPYTILEKDGIKIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGI
CCCCCCCEEEECCCCEEEEEEEEEEEEECCCHHHHEEECCCCCCCCCCCCCCCHHHHCCC
EARDEVKYLQHYIDELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGL
CHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCE
DILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYAD
EEEEECCCCCCCCCCEECCCEEEEEECCCCCEEEEEEEECCCCCCCEEEEEEEEEEEEEC
EWIPDPTTQKVINGWNKKLVDIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQ
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHEECCCEE
IALINSGSLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQM
EEEEECCCEEEECCCCEEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHH
SKGAEMRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQV
CCCCCEEEEHHCCCCCEEEEEEECCCEEECCCEEEEEEEEEEEECCCEEEEEECCCEEEE
RAGNNMSNVVIDYFKKGHKITPAQVNEMRVEVSK
EECCCHHHHHHHHHHCCCEECCCCCCEEEEEECC
>Mature Secondary Structure
MVKFMVAYCDSIKRHSIHNIDNTGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGP
CCEEEEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
YISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEK
HHHHHCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEECCHHHCCCCC
PFWNKPYTILEKDGIKIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGI
CCCCCCCEEEECCCCEEEEEEEEEEEEECCCHHHHEEECCCCCCCCCCCCCCCHHHHCCC
EARDEVKYLQHYIDELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGL
CHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCE
DILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYAD
EEEEECCCCCCCCCCEECCCEEEEEECCCCCEEEEEEEECCCCCCCEEEEEEEEEEEEEC
EWIPDPTTQKVINGWNKKLVDIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQ
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHEECCCEE
IALINSGSLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQM
EEEEECCCEEEECCCCEEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHH
SKGAEMRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQV
CCCCCEEEEHHCCCCCEEEEEEECCCEEECCCEEEEEEEEEEEECCCEEEEEECCCEEEE
RAGNNMSNVVIDYFKKGHKITPAQVNEMRVEVSK
EECCCHHHHHHHHHHCCCEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]