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The map label for this gene is metE [H]

Identifier: 218692105

GI number: 218692105

Start: 4450497

End: 4452758

Strand: Direct

Name: metE [H]

Synonym: ECED1_4514

Alternate gene names: 218692105

Gene position: 4450497-4452758 (Clockwise)

Preceding gene: 218692103

Following gene: 218692106

Centisome position: 85.43

GC content: 55.08

Gene sequence:

>2262_bases
ATGACAATTCTTAATCACACCCTCGGTTTCCCTCGCGTTGGCCTGCGTCGCGAGCTGAAAAAAGCGCAAGAGAGTTATTG
GGCGGGGAACTCCACGCGTGAAGAACTGCTGGCGGTAGGGCGTGAATTGCGTGCTCGTCACTGGGATCAACAAAAGCAAG
CGGGTATCGACCTGCTGCCGGTGGGCGATTTTGCCTGGTACGATCATGTACTGACCACCAGTCTGCTGCTGGGTAATGTT
CCGCCACGTCATCAGAACAACGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCACCGACTGG
CGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCC
AACAGTTCAAACTGACCTGGACGCAGCTGCTGGAGGAAGTGGACGAGGCGCTGGCGCTGGGCCACAAGGTGAAACCTGTG
CTGCTGGGGCCAGTTACCTACCTGTGGCTGGGTAAAGTGAAAGGTGAACAGTTTGATCGCCTGAGCCTGCTGAACGACAT
TCTGCCGGTTTATCAGCAAGTGCTGGCAGAACTGGCGAAACGCGGCATCGAGTGGGTACAGATTGATGAACCCGCGCTGG
TACTGGAACTGCCGCAGGCGTGGCTGGACGCATACAAACCCGCTTACGACGCGCTCCAGGGACAGGTGAAACTGCTGCTG
ACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTCGATCTCGT
ACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGTCTGCCTTCTGACTGGCTGCTGTCTGCGGGTCTTATCAATGGTC
GTAACGTCTGGCGCGCCGATCTTACCGAGAAATATGCGCAAATTAAGGACATTGTCGGCAAACGTGATTTGTGGGTGGCA
TCTTCCTGCTCATTGCTGCACAGCCCTATCGACCTGAGCGTGGAAACGCGTCTTGATGCAGAAGTGAAAAGCTGGTTTGC
CTTCGCCCTGCAAAAATGTCATGAACTGGCATTGCTGCGCGATGCGTTGAACAGTGGTGATACGGCAGCTCTGGCAGAGT
GGAGCGCTCCGATTCAGGCGCGTCGTCACTCTACTCGTGTACATAATCCGGCAGTAGAAAAGCGTCTGGCGGCGATCACC
GCTCAGGACAGTCAGCGTGCGAATGTCTATGAAGTGCGTGCTGAAGCCCAGCGTGCGCGTTTTAAACTGCCCGCGTGGCC
GACCACCACGATTGGTTCCTTCCCGCAAACCACGGAGATTCGTACCCTGCGTCTGGATTTTAAAAAGGGTAATCTCGACG
CCAATAACTACCGCACGGGCATTGCGGAACATATCAAGCAGGCCATTGTTGAGCAGGAACGTTTGGGACTGGATGTGCTG
GTACATGGCGAGGCCGAGCGTAATGACATGGTGGAATACTTTGGCGAGCATCTGGATGGCTTTGTCTTTACGCAAAACGG
TTGGGTACAGAGCTACGGTTCCCGCTGCGTGAAGCCACCGATTGTTATTGGTGACGTTAGCCGCCCGGCACCGATTACCG
TGGAGTGGGCAAAATATGCGCAATCCCTGACTGATAAACCGGTGAAAGGGATGTTGACCGGCCCGGTGACTATTCTCTGC
TGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAAATTGCGCTGGCGCTGCGTGATGAAGTCGCGGA
CCTGGAAGCCGCTGGAATTGGCATCATTCAGATTGACGAACCGGCATTGCGCGAAGGTTTACCACTGCGTCGCAGCGACT
GGGATGCCTATCTCCAGTGGGGCGTGGAGGCTTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACT
CACATGTGTTACTGCGAGTTCAACGACATCATGTATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTC
GCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTC
ACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGTCTG
TGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGC
GCAGAATTTGCGTCGGGGGTAA

Upstream 100 bases:

>100_bases
GCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCCGGACGGCTAAATACTAAA
AATTCCATAAGGGGCATATA

Downstream 100 bases:

>100_bases
AATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGGCGTCTTCCCAGCATACTGCGTAAACCATTCCA
GATATCAGTTCATACCCTGT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 753; Mature: 752

Protein sequence:

>753_residues
MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNV
PPRHQNNDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL
TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVA
SSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAIT
AQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL
VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILC
WSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHT
HMCYCEFNDIMYSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL
WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG

Sequences:

>Translated_753_residues
MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNV
PPRHQNNDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL
TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVA
SSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAIT
AQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL
VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILC
WSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHT
HMCYCEFNDIMYSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL
WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG
>Mature_752_residues
TILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVP
PRHQNNDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPVL
LGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLLT
TYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVAS
SCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITA
QDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVLV
HGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCW
SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTH
MCYCEFNDIMYSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLW
VNPDCGLKTRGWPETRAALANMVQAAQNLRRG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=753, Percent_Identity=99.203187250996, Blast_Score=1534, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=761, Percent_Identity=49.1458607095926, Blast_Score=713, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 84711; Mature: 84580

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEE
VGDFAWYDHVLTTSLLLGNVPPRHQNNDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFN
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC
TNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPVLLGPVTYLWLGKVKGEQFDR
CCCEEECHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHCCCCCHHHH
LSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHCHHHHHHHHHHHHHH
TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRAD
HHHHCCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHCCHHHHHHCCCCCCCCCCHHH
LTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLR
HHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHH
DALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVRAEAQRAR
HHHCCCCCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
FKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL
EECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYA
EECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCCEEEHHHHHH
QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE
HHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
PALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMYSIAALDAD
HHHHCCCCCCCCCHHHHHHHCCHHEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCE
VITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL
EEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE
WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEE
VGDFAWYDHVLTTSLLLGNVPPRHQNNDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFN
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC
TNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPVLLGPVTYLWLGKVKGEQFDR
CCCEEECHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHCCCCCHHHH
LSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHCHHHHHHHHHHHHHH
TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRAD
HHHHCCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHCCHHHHHHCCCCCCCCCCHHH
LTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLR
HHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHH
DALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVRAEAQRAR
HHHCCCCCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
FKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL
EECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYA
EECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCCEEEHHHHHH
QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE
HHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
PALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMYSIAALDAD
HHHHCCCCCCCCCHHHHHHHCCHHEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCE
VITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL
EEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE
WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA