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The map label for this gene is tnaB [H]

Identifier: 218691998

GI number: 218691998

Start: 4327746

End: 4328993

Strand: Direct

Name: tnaB [H]

Synonym: ECED1_4400

Alternate gene names: 218691998

Gene position: 4327746-4328993 (Clockwise)

Preceding gene: 218691997

Following gene: 218691999

Centisome position: 83.07

GC content: 45.91

Gene sequence:

>1248_bases
ATGACTGATCAATCAGAAAAAAAACACTCTGCATTTTGGGGTGTTATGGTTATAGCCGGTACAGTAATTGGCGGTGGGAT
GTTTGCTTTACCTGTTGACCTTGCCGGAGCCTGGTTTTTTTGGGGCGCTTTTATTCTTATTATCGCCTGGTTTTCAATGT
TACATTCAGGGCTATTATTATTAGAAGCAAACTTAAATTATCCCGTCGGTTCCAGTTTTAACACCATCACCAAAGATTTA
ATCGGTAACACCTGGAACATTATCAGCGGTATTACCGTTGCCTTCGTTCTCTATATCCTCACTTATGCCTATATCTCTGC
TAATGGTGCGATCATTAGTGAAACGATATCAATGAATTTGGGCTATCACGCTAATCCACGTATTGTCGGGATCTGCACTG
CCATTTTCGTTGCCAGCGTATTGTGGATAAGTTCGTTAGCCGCCAGTCGTATTACCTCATTGTTCCTTGGGCTGAAGATT
ATCTCCTTTGTAATTGTTTTTGGTTCTTTCTTCTTCCAGGTCGATTACTCCATTCTGCGCGATTCCACCAGCACCACTGC
GGGGACGTCTTACTTCCCGTATATCTTTATGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCA
GCCTGATTATTTGCTATGGCAAAAAGAAAGACAAACTAATCAAAAGCGTGGTCTTCGGTTCGCTGCTGGCGCTGGTGATT
TATCTCTTCTGGCTCTATTGCACGATGGGGAATATCCCGCGAGAAAGCTTTAAGGCGATAATCTCCTCAGGCGGCAACGT
TGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGGATTATCGAGTTTTGCCTGCTGGTGTTCTCCAACTTAG
CTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTATCTGGCGGATCTGTTTAAGATTGATAACTCCCAC
GGCGGGCGTTTCAAAACCGTGCTGTTAACCTTCCTGCCACCTGCTTTGTTGTATCTGATCTTCCCGAACGGCTTCATTTA
CGGGATCGGCGGTGCCGGACTGTGTGCCACCATCTGGGCGGTAATAATTCCTGCTGTACTGGCAATCAAAGCCCGCAAGA
AGTTTCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGCATAACCGTAATT
TTATGCTGGTTCGGTAACGTCTTTAACGTGTTACCTAAATTTGGCTAA

Upstream 100 bases:

>100_bases
GAAGTTTAATTAAAACACTCAGAGTGGCTATAAGGATGTTAGCCACTCTATTACCCTACATCCTCAATAACAAAAATAGC
CTTCCTCTAAAGGTGGCATC

Downstream 100 bases:

>100_bases
ATCCTTCAGGAAGCCAGCCATTCGCTGGCTTCTTGCCTGTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATTC
TCCTTCACCACGTATGCTTT

Product: tryptophan permease TnaB

Products: L-tryptophan [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDL
IGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKKKDKLIKSVVFGSLLALVI
YLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSH
GGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVI
LCWFGNVFNVLPKFG

Sequences:

>Translated_415_residues
MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDL
IGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKKKDKLIKSVVFGSLLALVI
YLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSH
GGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVI
LCWFGNVFNVLPKFG
>Mature_414_residues
TDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLI
GNTWNIISGITVAFVLYILTYAYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKII
SFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKKKDKLIKSVVFGSLLALVIY
LFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHG
GRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVIL
CWFGNVFNVLPKFG

Specific function: Involved in tryptophan transport across the cytoplasmic membrane. Plays a role in transporting tryptophan which is to be used catabolically [H]

COG id: COG0814

COG function: function code E; Amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid/polyamine transporter 2 family. Mtr/TnaB/TyrP permease subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790145, Length=415, Percent_Identity=98.7951807228916, Blast_Score=815, Evalue=0.0,
Organism=Escherichia coli, GI1789552, Length=388, Percent_Identity=52.8350515463918, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1788218, Length=411, Percent_Identity=33.0900243309002, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002091
- InterPro:   IPR013061
- InterPro:   IPR013059
- InterPro:   IPR018227 [H]

Pfam domain/function: PF03222 Trp_Tyr_perm [H]

EC number: NA

Molecular weight: Translated: 45229; Mature: 45098

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00594 AROMATIC_AA_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLL
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNL
EEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCC
GYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKIISFVIVFGSFFFQVDYSILR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKKKDKLIKSVVFGSLLALVI
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
YLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFF
HHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
VIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLL
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNL
EEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCC
GYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKIISFVIVFGSFFFQVDYSILR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKKKDKLIKSVVFGSLLALVI
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
YLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFF
HHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
VIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-tryptophan [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-tryptophan [Periplasm] + Proton [Periplasm] = L-tryptophan [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]