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The map label for this gene is tnaA

Identifier: 218691997

GI number: 218691997

Start: 4326239

End: 4327654

Strand: Direct

Name: tnaA

Synonym: ECED1_4399

Alternate gene names: 218691997

Gene position: 4326239-4327654 (Clockwise)

Preceding gene: 218691996

Following gene: 218691998

Centisome position: 83.04

GC content: 50.85

Gene sequence:

>1416_bases
ATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCG
TGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTGTTTATCGATTTACTGACCGACA
GCGGCACCGGGGCGGTGACGCAGAGTATGCAGGCCGCGATGATGCGCGGCGACGAAGCTTACAGCGGCAGCCGCAGCTAC
TACGCGTTAGCCGAGTCAGTGAAAAATATCTTTGGTTATCAATACACTATTCCGACTCACCAGGGCCGTGGTGCAGAGCA
AATCTATATTCCGGTACTGATTAAAAAACGCGAGCAGGAAAAAGGCCTGGATCGCAGCAAAATGGTGGCGTTCTCTAACT
ATTTCTTTGATACCACGCAGGGCCATAGCCAGATTAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGAT
ACTGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAACGCGGTATTGAAGAAGTTGGCCCGAATAA
CGTGCCGTATATCGTTGCAACCATCACCAGTAACTCCGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGT
ACAGCATCGCGAAGAAATACGATATTCCGGTGGTCATGGACTCCGCGCGCTTTGCCGAAAACGCCTATTTCATCAAGCAG
CGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGCCGATATGCTGGCGATGTC
CGCCAAGAAAGATGCGATGGTGCCGATGGGCGGGCTGCTGTGCATGAAAGACGACAGCTTCTTTGATGTGTATACCGAAT
GCAGAACCCTTTGCGTGGTGCAGGAAGGCTTCCCGACATATGGCGGCCTGGAAGGCGGCGCTATGGAGCGTCTGGCGGTA
GGTCTGTATGACGGCATGAATCTGGACTGGCTGGCTTATCGTATCGCGCAGGTGCAGTATCTGGTCGATGGTCTGGAAGA
GATTGGCGTTGTCTGTCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCAGGTAAACTGTTGCCGCATATCCCGGCAG
ATCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGTATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTG
TTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACATA
TACTCAAACACATATGGACTTCATTATTGAAGCCTTTAAACATGTGAAAGAGAACGCGGCGAATATTAAAGGGTTAACCT
TTACCTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTGAAAGAAGTTTAA

Upstream 100 bases:

>100_bases
TAAATGATGGTGCTTGCATATATATCTGGCGAATTAATGGATACTGCAAATGTAATATTCACAGGGATCACTGTAATTAA
AATAAATGAAGGATTATGTA

Downstream 100 bases:

>100_bases
TTAAAACACTCAGAGTGGCTATAAGGATGTTAGCCACTCTATTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTA
AAGGTGGCATCATGACTGAT

Product: tryptophanase

Products: NA

Alternate protein names: L-tryptophan indole-lyase; TNase

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSY
YALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQ
REAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAV
GLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFL
LGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV

Sequences:

>Translated_471_residues
MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSY
YALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQ
REAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAV
GLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFL
LGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV
>Mature_471_residues
MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSY
YALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQ
REAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAV
GLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFL
LGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family

Homologues:

Organism=Escherichia coli, GI87082323, Length=471, Percent_Identity=100, Blast_Score=982, Evalue=0.0,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TNAA_ECO24 (A7ZTR3)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001465192.1
- ProteinModelPortal:   A7ZTR3
- SMR:   A7ZTR3
- STRING:   A7ZTR3
- EnsemblBacteria:   EBESCT00000021377
- GeneID:   5587073
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4217
- eggNOG:   COG3033
- GeneTree:   EBGT00050000011364
- HOGENOM:   HBG297784
- OMA:   IYFSARK
- ProtClustDB:   PRK13238
- BioCyc:   ECOL331111:ECE24377A_4217-MONOMER
- HAMAP:   MF_00544
- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- InterPro:   IPR013440
- InterPro:   IPR018176
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF001386
- TIGRFAMs:   TIGR02617

Pfam domain/function: PF01212 Beta_elim_lyase; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.1.99.1

Molecular weight: Translated: 52774; Mature: 52774

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00853 BETA_ELIM_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDVFIDLLTDSGTGAVT
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCHHH
QSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEEEEECCHHH
KGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDFKGNFDLEGLERGI
CCCCHHHEEEEHHCEEECCCCCCCCCCEEEEEEEEEHHHCCCCEEEECCCCCHHHHHHHH
EEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQ
HHHCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCEEECCHHHHCCEEEEEC
REAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEEE
QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAA
ECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
FVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAELLRL
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHE
TIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure
MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDVFIDLLTDSGTGAVT
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCHHH
QSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEEEEECCHHH
KGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDFKGNFDLEGLERGI
CCCCHHHEEEEHHCEEECCCCCCCCCCEEEEEEEEEHHHCCCCEEEECCCCCHHHHHHHH
EEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQ
HHHCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCEEECCHHHHCCEEEEEC
REAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEEE
QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAA
ECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
FVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAELLRL
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHE
TIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA