Click here to switch to the map view.

The map label for this gene is tiaE

Identifier: 218691844

GI number: 218691844

Start: 4159930

End: 4160904

Strand: Direct

Name: tiaE

Synonym: ECED1_4238

Alternate gene names: 218691844

Gene position: 4159930-4160904 (Clockwise)

Preceding gene: 218691843

Following gene: 218691846

Centisome position: 79.85

GC content: 51.59

Gene sequence:

>975_bases
ATGAAGCCGTCCGTTATCCTCTACAAAGCCTTACCTGATGATTTACTGCAACGCCTGCAAGAACATTTCACCGTTCACCA
GGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGCTTGCTGGGTTCAAATG
AGAATGTTGATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTT
GATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACACCAACCGTATTAACAGAAACCGTCGCCGATACGCT
GATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATGGACCGCGAGCA
TAGGCCCGGACTGGTACGGCACTGACGTTCACCATAAAACACTGGGCATTGTCGGGATGGGACGGATCGGCATGGCGCTG
GCACAACGTGCGCACTTTGGCTTCAACATGCCCATCCTCTATAACGCGCGTCGCCACCATAAAGAAGCAGAAGAACGCTT
CAATGCCCGCTACTGCGATTTGGATACTCTGTTACAAGAGTCAGATTTCGTTTGCCTGATCCTGCCGTTAACGGATGAGA
CGCATCATCTATTTGGCGCAGAACAATTCGCCAAAATGAAATCCTCCGCCATTTTCATTAATGCCGGACGTGGCCCGGTG
GTTGATGAAAATGCACTGATTGCTGCATTGCAGAAAGGGGAAATTCACGCCGCCGGGCTGGATGTCTTCGAACAAGAGCC
ACTGTCCGTAGATTCGCCGTTGCTCTCAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACTCATGAGACGC
GTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCG
CACGTCGCGGACTAA

Upstream 100 bases:

>100_bases
CTTTCATGTCAGGTGATGCAGAAATCACCTGGCATTCTTCAGTTTTCCTTCATCATATTTCAGGCTAAGGTGATCGCCTT
ATCAGTGAATGGAGAGAAGC

Downstream 100 bases:

>100_bases
GCCGCGACTGCGTGGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAG
GCCTGATTAAACGCCTGATG

Product: 2-oxo-carboxylic acid reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNF
DVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNP
HVAD

Sequences:

>Translated_324_residues
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNF
DVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNP
HVAD
>Mature_324_residues
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNF
DVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNP
HVAD

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily

Homologues:

Organism=Homo sapiens, GI6912396, Length=273, Percent_Identity=42.1245421245421, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI23308577, Length=312, Percent_Identity=30.7692307692308, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI145580578, Length=268, Percent_Identity=32.089552238806, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI4557499, Length=268, Percent_Identity=32.089552238806, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI145580575, Length=274, Percent_Identity=32.4817518248175, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI61743967, Length=270, Percent_Identity=32.2222222222222, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4557497, Length=270, Percent_Identity=32.2222222222222, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI87082289, Length=324, Percent_Identity=99.6913580246914, Blast_Score=669, Evalue=0.0,
Organism=Escherichia coli, GI1787645, Length=269, Percent_Identity=27.5092936802974, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789279, Length=249, Percent_Identity=30.9236947791165, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081824, Length=258, Percent_Identity=27.5193798449612, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=250, Percent_Identity=33.6, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=274, Percent_Identity=30.2919708029197, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=243, Percent_Identity=38.6831275720165, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320925, Length=256, Percent_Identity=32.421875, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=248, Percent_Identity=31.4516129032258, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6325144, Length=198, Percent_Identity=31.3131313131313, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=240, Percent_Identity=27.0833333333333, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6321253, Length=279, Percent_Identity=24.7311827956989, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45551003, Length=312, Percent_Identity=39.1025641025641, Blast_Score=204, Evalue=8e-53,
Organism=Drosophila melanogaster, GI45552429, Length=312, Percent_Identity=39.1025641025641, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24585514, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI28574282, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI28574284, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI28574286, Length=315, Percent_Identity=37.7777777777778, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28571528, Length=261, Percent_Identity=41.7624521072797, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24585516, Length=247, Percent_Identity=36.0323886639676, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24646446, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646448, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646452, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646450, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI62472511, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921140, Length=251, Percent_Identity=30.6772908366534, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GHRB_ECO24 (A7ZTA0)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001465029.1
- ProteinModelPortal:   A7ZTA0
- SMR:   A7ZTA0
- STRING:   A7ZTA0
- EnsemblBacteria:   EBESCT00000019757
- GeneID:   5590663
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4049
- eggNOG:   COG1052
- GeneTree:   EBGT00050000009079
- HOGENOM:   HBG731446
- OMA:   WIKGAGL
- ProtClustDB:   PRK15409
- BioCyc:   ECOL331111:ECE24377A_4049-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01667
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.79; =1.1.1.81

Molecular weight: Translated: 35397; Mature: 35397

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 237-237 ACT_SITE 266-266 ACT_SITE 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAAL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCCCCHHHH
LEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVE
HHHCCHHCCCEEEEECCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNARRHH
HHHHHHCCCEEECCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHCCCCCCEEECCHHHH
KEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
HHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHHCCCEEEEECCCCCC
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAAC
CCCCHHEEEHHCCCEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AVDNLIDALQGKVEKNCVNPHVAD
HHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAAL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCCCCHHHH
LEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVE
HHHCCHHCCCEEEEECCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNARRHH
HHHHHHCCCEEECCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHCCCCCCEEECCHHHH
KEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
HHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHHCCCEEEEECCCCCC
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAAC
CCCCHHEEEHHCCCEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AVDNLIDALQGKVEKNCVNPHVAD
HHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA