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The map label for this gene is uspF

Identifier: 218689336

GI number: 218689336

Start: 1530821

End: 1531255

Strand: Reverse

Name: uspF

Synonym: ECED1_1558

Alternate gene names: 218689336

Gene position: 1531255-1530821 (Counterclockwise)

Preceding gene: 218689337

Following gene: 218689335

Centisome position: 29.39

GC content: 48.28

Gene sequence:

>435_bases
ATGAACAGAACGATTCTTGTCCCTATCGATATTTCCGATTCAGAATTAACTCAACGCGTGATTAGCCACGTTGAGGCAGA
GGCAAAGATTGATGATGCAGAGGTTCATTTCCTGACGGTAATACCTTCGCTGCCCTACTATGCCTCTCTGGGTTTAGCCT
ATTCCGCAGAATTACCGGCAATGGATGATCTGAAAGCGGAAGCCAAATCGCAACTGGAAGAGATCATTAAAAAATTTAAA
CTGCCAACCGACAGAGTGCATGTCCATGTTGAAGAAGGCTCGCCCAAAGACCGCATTCTGGAATTGGCGAAGAAGATCCC
CGCTCATATGATCATCATTGCTTCCCATCGACCGGATATCACCACTTATCTGCTCGGTTCTAACGCCGCAGCTGTAGTGC
GTCACGCAGAGTGCTCCGTGCTGGTTGTGCGCTGA

Upstream 100 bases:

>100_bases
TCTTCCTCCTTTGATCTAAATTAAAAATGTGAACTCCGTCATTACACAAAAAGTGTCATCTGGCGTTACACTTTATGCGG
ATACTAAAACAGGAGGTTTT

Downstream 100 bases:

>100_bases
CACTAACGCCCGCACATTGCTGCGGGCTTTTTGATTCATTTCGCAAATGTGCTGACATTTTCCCCTCTAATCATTACAAT
TTAAATTTATGAATTATGCA

Product: stress-induced protein, ATP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFK
LPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR

Sequences:

>Translated_144_residues
MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFK
LPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR
>Mature_144_residues
MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFK
LPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR

Specific function: Unknown

COG id: COG0589

COG function: function code T; Universal stress protein UspA and related nucleotide-binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the universal stress protein A family

Homologues:

Organism=Escherichia coli, GI87081893, Length=144, Percent_Identity=99.3055555555556, Blast_Score=286, Evalue=4e-79,
Organism=Escherichia coli, GI1786824, Length=146, Percent_Identity=39.041095890411, Blast_Score=100, Evalue=4e-23,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): USPF_ECO57 (P0A4P7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E85740
- PIR:   E90878
- RefSeq:   NP_287771.1
- RefSeq:   NP_310024.2
- ProteinModelPortal:   P0A4P7
- SMR:   P0A4P7
- EnsemblBacteria:   EBESCT00000026959
- EnsemblBacteria:   EBESCT00000059753
- GeneID:   917194
- GeneID:   961019
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2335
- KEGG:   ecs:ECs1997
- GeneTree:   EBGT00050000010679
- HOGENOM:   HBG733992
- OMA:   HVVRYAK
- ProtClustDB:   PRK15005
- BioCyc:   ECOL83334:ECS1997-MONOMER
- InterPro:   IPR014729
- InterPro:   IPR006015
- InterPro:   IPR006016
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01438

Pfam domain/function: PF00582 Usp

EC number: NA

Molecular weight: Translated: 15959; Mature: 15959

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPA
CCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHCCEEEECCCCC
MDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDI
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCE
TTYLLGSNAAAVVRHAECSVLVVR
EEEEECCCCEEEEEECCEEEEEEC
>Mature Secondary Structure
MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPA
CCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHCCEEEECCCCC
MDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDI
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCE
TTYLLGSNAAAVVRHAECSVLVVR
EEEEECCCCEEEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796