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The map label for this gene is dhaH [H]

Identifier: 218689145

GI number: 218689145

Start: 1333425

End: 1334843

Strand: Reverse

Name: dhaH [H]

Synonym: ECED1_1346

Alternate gene names: 218689145

Gene position: 1334843-1333425 (Counterclockwise)

Preceding gene: 218689146

Following gene: 218689144

Centisome position: 25.62

GC content: 50.11

Gene sequence:

>1419_bases
ATGGTAAACCTGGTCATAGTTTCACATAGCAGCCGACTGGGAGAAGGTGTCGGTGAATTAGCCCGTCAGATGTTAATGAG
TGATAGTTGTAAAATCGCCATTGCCGCGGGAATTGACGATCCACATAATCCCATTGGTACCGATGCCGTCAAAGTGATGG
AGGCCATCGAATCTGTTGCCGATGCCGACCATGTGCTGGTCATGATGGATATGGGTAGCGCATTATTGAGTGCTGAAACT
GCGCTGGAATTGCTGGCTCCCGAGATCGCCGCAAAAGTACGTTTGTGTGCTGCGCCGTTGGTCGAAGGTACACTGGCAGC
TACGGTCAGCGCGGCCTCGGGGGCGGATATCGACAAAGTTATCTTTGACGCCATGCATGCGCTGGAAGCCAAACGTGAAC
AACTGGGTTTACCGTCCTCCGACACTGAAATCTCTGACACATGTCCTCCGTACGATGAAGAAGCCCGTTCTCTGTCGGTG
GTCATAAAAAACCGTAACGGCCTGCATGTACGTCCGGCCTCCCGGCTGGTTTATACCTTATCGACATTTAATGCCGATAT
GTTGCTGGAGAAAAACGGCAAATGCGTTACACCGGATAGTATTAACCAGATTGCGTTACTACAAGTTCGCTATAACGATA
CGCTGCGCCTGATTGCGAAAGGACCAGAAGCTGAAGAGGCACTGATCGCTTTCCGTCAGCTGGCTGAAGATAACTTTGGT
GAAACGGAGGAAGTTGCTCCACCTACTCTGCGTCCCGTTCCGCCTGTTTCGGGTAAAGCCTTTTATTATCAACCTGTTTT
ATGTACGGTACAGGCAAAATCAACCCTGACCGTGGAAGAAGAACAAGAACGATTACGCCAGGCCATTGACTTCACGTTAT
TAGATCTGATGACGTTAACAGCGAAAGCAGAAACCTGCGGGCTTGACGATATTGCCGCAATCTTTTCTGGTCACCATACA
CTGTTAGATGATCCGGAACTGCAGGCGGCGGCAAGCGAACTCCTTCAGCATGAACATTGCACGGCGGAATATGCCTGGCA
GCAAGTTCTTAAAGAACTTAGCCAGCAATACCAACAGCTGGATGATGAATATCTACAGGCTCGCTATATCGATGTGGACG
ATCTTCTGCATCGCACCCTGGTCCACCTGACCCAAACGAAAGAAGAACTCCCGCAGTTTAACTCGCCAACTATTCTACTG
GCGGAGAACATTTATCCCTCCACAGTACTGCAACTGGATCCAGCGGTTGTAAAAGGTATCTGCCTTAGCGCCGGAAGTCC
GCTATCCCACAGTGCCCTAATCGCCCGTGAACTGGGAATTGGCTGGATTTGCCAGCAGGGTGAGAAACTGTATGCGATAC
AACCAGAAGAAACGCTAACGCTGGACGTTAAAACGCAACGTTTCAACCGTCAGGGTTAA

Upstream 100 bases:

>100_bases
CTCGGTGAACGCAGTATTGGTCACCAGGATCCCGGCGCGACCTCGGTGATGTTTATGATGCAAATGTTGGCGTTAGCCGC
AAAAGAGTAAGGAATTGGTG

Downstream 100 bases:

>100_bases
ACCACCGTTCGGATGGCATCATTCTGATGTCATCCGGCAATTTACTCTGCACTGATGTTTTTAACTACCTGTTATATTTT
TCACCAAATTATGATGATTT

Product: dihydroxyacetone kinase subunit M

Products: NA

Alternate protein names: Phosphocarrier protein HPr; Protein H; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPHNPIGTDAVKVMEAIESVADADHVLVMMDMGSALLSAET
ALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPDSINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTLTVEEEQERLRQAIDFTLLDLMTLTAKAETCGLDDIAAIFSGHHT
LLDDPELQAAASELLQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILL
AENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG

Sequences:

>Translated_472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPHNPIGTDAVKVMEAIESVADADHVLVMMDMGSALLSAET
ALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPDSINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTLTVEEEQERLRQAIDFTLLDLMTLTAKAETCGLDDIAAIFSGHHT
LLDDPELQAAASELLQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILL
AENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG
>Mature_472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPHNPIGTDAVKVMEAIESVADADHVLVMMDMGSALLSAET
ALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPDSINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTLTVEEEQERLRQAIDFTLLDLMTLTAKAETCGLDDIAAIFSGHHT
LLDDPELQAAASELLQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILL
AENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG

Specific function: Phosphotransferase subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. DhaM serves as the phosphoryl donor. It is phosphorylated by HPr, then it donates its phosphoryl group to dhaL-ADP, which eventually tran

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI226510935, Length=472, Percent_Identity=98.0932203389831, Blast_Score=946, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=187, Percent_Identity=33.1550802139037, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789193, Length=178, Percent_Identity=28.0898876404494, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012844
- InterPro:   IPR008279
- InterPro:   IPR004701
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731 [H]

Pfam domain/function: PF03610 EIIA-man; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 51575; Mature: 51575

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00369 PTS_HPR_HIS ; PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPHNPIGTDAVKVMEAIESVA
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
DADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGADIDKV
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCHHHH
IFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSVVIKNRNGLHVRPASRLVYTL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCEEECCHHHEEEEE
STFNADMLLEKNGKCVTPDSINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ECCCCEEEEECCCCEECCCCCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCCCC
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTLTVEEEQERLRQAIDFTLLDLMTLT
CCCCCCCCCCCCCCCCCCCEEEEECEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHH
AKAETCGLDDIAAIFSGHHTLLDDPELQAAASELLQHEHCTAEYAWQQVLKELSQQYQQL
HHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILLAENIYPSTVLQLDPAVVKGI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHEEECHHHHHHH
CLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG
HHCCCCCCCHHHHHHHHCCCCEEECCCCEEEEECCCCEEEEEEEHHHCCCCC
>Mature Secondary Structure
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPHNPIGTDAVKVMEAIESVA
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
DADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGADIDKV
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCHHHH
IFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSVVIKNRNGLHVRPASRLVYTL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCEEECCHHHEEEEE
STFNADMLLEKNGKCVTPDSINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ECCCCEEEEECCCCEECCCCCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCCCC
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTLTVEEEQERLRQAIDFTLLDLMTLT
CCCCCCCCCCCCCCCCCCCEEEEECEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHH
AKAETCGLDDIAAIFSGHHTLLDDPELQAAASELLQHEHCTAEYAWQQVLKELSQQYQQL
HHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILLAENIYPSTVLQLDPAVVKGI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHEEECHHHHHHH
CLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG
HHCCCCCCCHHHHHHHHCCCCEEECCCCEEEEECCCCEEEEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2671658; 7984428 [H]