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The map label for this gene is entB [H]

Identifier: 218688421

GI number: 218688421

Start: 616334

End: 617191

Strand: Direct

Name: entB [H]

Synonym: ECED1_0592

Alternate gene names: 218688421

Gene position: 616334-617191 (Clockwise)

Preceding gene: 218688420

Following gene: 218688422

Centisome position: 11.83

GC content: 55.13

Gene sequence:

>858_bases
ATGGCTATTCCAAAATTACAGGCTTACGCACTGCCGGAGTCTCACGATATTCCGCACAATAAAGTCGACTGGGCCTTTGA
ACCGCAACGTGCCGCGTTGTTAATCCATGATATGCAGGACTATTTTGTCAGCTTCTGGGGTGAGAACTGCCCGATGATGG
AGCAGGTGATTGCGAATATTGCTGCGCTACGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCG
AAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGCCAGGCCTGACCCGTTCGCCGGAACAGCAAAA
GGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCCCCGC
TGGAGCAAATGCTCAAAGAGAGTGGACGTAACCAGCTGATTATTACCGGGGTATATGCCCACATTGGCTGTATGACCACC
GCAACGGACGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGATGCGCTGGCCGATTTCAGCCGTGACGAGCATTT
GATGTCGCTGAAATATGTGGCCGGACGTTCTGGTCGGGTGGTGATGACTGAAGAATTACTGCCAGCACCTGTCCCCGCCA
GCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGAC
TACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGCGATATCGACTTTGTCATGCT
GGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAA

Upstream 100 bases:

>100_bases
TGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCACATC
AGCCTGAAGGAGAGAACACG

Downstream 100 bases:

>100_bases
TGGATTTCAGCGGTAAAAATGTCTGGGTAACTGGCGCGGGGAAAGGTATCGGCTATGCCACGGCGCTGGCATTTGTTGAG
GCGGGGGCGAAAGTTACAGG

Product: isochorismatase

Products: NA

Alternate protein names: 2,3 dihydro-2,3 dihydroxybenzoate synthase; Enterobactin synthase component B; Enterochelin synthase B; Isochorismate lyase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MAIPKLQAYALPESHDIPHNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQP
KEQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAALREVILPLLDESDEPFDDDNLID
YGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREVK

Sequences:

>Translated_285_residues
MAIPKLQAYALPESHDIPHNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQP
KEQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAALREVILPLLDESDEPFDDDNLID
YGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREVK
>Mature_284_residues
AIPKLQAYALPESHDIPHNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPK
EQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTTA
TDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAALREVILPLLDESDEPFDDDNLIDY
GLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREVK

Specific function: Required for production of 2,3-DHB. Also serves as an aryl carrier protein and plays a role in enterobactin assembly [H]

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=285, Percent_Identity=99.2982456140351, Blast_Score=588, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009081
- InterPro:   IPR016291
- InterPro:   IPR000868
- InterPro:   IPR006163 [H]

Pfam domain/function: PF00857 Isochorismatase; PF00550 PP-binding [H]

EC number: =3.3.2.1 [H]

Molecular weight: Translated: 32550; Mature: 32418

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPKLQAYALPESHDIPHNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANI
CCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTV
HHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEE
LVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTTATDAFMRDIKPFMVADALAD
EEEEEHHHHHCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAALREVILPLLDESDEPFDDDNLID
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
YGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREVK
HCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AIPKLQAYALPESHDIPHNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANI
CCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTV
HHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEE
LVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTTATDAFMRDIKPFMVADALAD
EEEEEHHHHHCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAALREVILPLLDESDEPFDDDNLID
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
YGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREVK
HCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]