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The map label for this gene is entE [H]

Identifier: 218688420

GI number: 218688420

Start: 614710

End: 616320

Strand: Direct

Name: entE [H]

Synonym: ECED1_0591

Alternate gene names: 218688420

Gene position: 614710-616320 (Clockwise)

Preceding gene: 218688419

Following gene: 218688421

Centisome position: 11.8

GC content: 56.49

Gene sequence:

>1611_bases
ATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCACT
GACCGACATTCTGACTCGCCACGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGGCAGTTGAGTTATCGGGAGC
TGAATCAGGCGGCGGATAACCTCGCCTGTAGTTTGCGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTG
GGTAACGTCGCTGAACTTTACATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGCTGTTCAGCCA
TCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCGGCGTTACTGATTGCCGATCGCCAACATGCGCTATTTA
GCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCCATTCGCGTGGTGCAGCTGCTCAACGACAGCGGTGAG
CATAACTTGCAGGATGCGATTAACCATCCGGCTGAGGATTTTACTGCCACGCCATCTCCTGCTGATGAAGTGGCCTATTT
CCAGCTTTCCGGCGGCACCACCGGCACTCCGAAACTGATCCCGCGCACTCATAACGACTACTACTACAGCGTGCGTCGTA
GCGTCGAGATTTGTCAGTTCACACAACAGACGCGCTACCTGTGCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCA
CCGGGATCGTTGGGGGTATTTCTCGCTGGCGGCACTGTCGTTCTGGCTGCCGACCCCAGCGCCACGCTTTGCTTCCCATT
GATTGAAAAACATCAGGTGAACGTCACCGCGCTGGTGCCGCCAGCAGTCAGCCTGTGGTTGCAGGCACTGGCTGAAGGCG
AAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCCCGTCTTTCTGCCACGCTTGCGGCGCGTATT
CCCGCTGAGATTGGCTGCCAGTTGCAGCAGGTGTTTGGCATGGCGGAAGGGCTGGTGAACTACACCCGTCTTGATGATAG
TGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGCCCGGACGATGAAGTATGGGTTGCCGATGCCGAAGGAAATC
CACTGCCGCAAGGGGAAGTTGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGTGGCTATTACAAAAGCCCGCAGCAC
AATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACTGT
GCAGGGGCGCGAGAAAGATCAGATCAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCC
ATCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGTGCTTATCTGGTGGTA
AAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATATAAATTACCGGATCGCGT
GGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCA
CATCAGCCTGA

Upstream 100 bases:

>100_bases
GGGGATTGTGCCTGCGTCCTCACCGTTGGGTGAGTGGCGCGAAACGGGCGTCAAACTTTCTACCATGTTGAACGTTTTTG
GATTGCATTAAGGAGCGAGG

Downstream 100 bases:

>100_bases
AGGAGAGAACACGATGGCTATTCCAAAATTACAGGCTTACGCACTGCCGGAGTCTCACGATATTCCGCACAATAAAGTCG
ACTGGGCCTTTGAACCGCAA

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEYKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRTSA

Sequences:

>Translated_536_residues
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEYKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRTSA
>Mature_535_residues
SIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQLG
NVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGEH
NLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSSP
GSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIP
AEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHN
ASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVVK
EPLRAVQVRRFLREQGIAEYKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRTSA

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=505, Percent_Identity=24.3564356435644, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI122937307, Length=510, Percent_Identity=24.1176470588235, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI42544132, Length=522, Percent_Identity=22.9885057471264, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI157311624, Length=518, Percent_Identity=23.7451737451737, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI157311622, Length=518, Percent_Identity=23.7451737451737, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI58082049, Length=518, Percent_Identity=24.1312741312741, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI156151445, Length=189, Percent_Identity=31.2169312169312, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI187761345, Length=402, Percent_Identity=24.8756218905473, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI187761343, Length=402, Percent_Identity=24.8756218905473, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI115511026, Length=511, Percent_Identity=22.7005870841487, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI28416953, Length=528, Percent_Identity=21.5909090909091, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI57165412, Length=382, Percent_Identity=24.3455497382199, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI57165410, Length=382, Percent_Identity=23.5602094240838, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI8923896, Length=547, Percent_Identity=20.109689213894, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI253970489, Length=373, Percent_Identity=22.5201072386059, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=99.2537313432836, Blast_Score=1101, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=534, Percent_Identity=31.2734082397004, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI221142682, Length=491, Percent_Identity=26.2729124236253, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788107, Length=537, Percent_Identity=25.1396648044693, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1790505, Length=541, Percent_Identity=24.2144177449168, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786801, Length=562, Percent_Identity=25.0889679715303, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786529, Length=529, Percent_Identity=23.4404536862004, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789201, Length=360, Percent_Identity=22.5, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=534, Percent_Identity=27.1535580524345, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=547, Percent_Identity=26.691042047532, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=504, Percent_Identity=23.8095238095238, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=525, Percent_Identity=24.1904761904762, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=531, Percent_Identity=24.105461393597, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994703, Length=519, Percent_Identity=23.6994219653179, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71994694, Length=519, Percent_Identity=23.6994219653179, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71994690, Length=519, Percent_Identity=23.6994219653179, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=505, Percent_Identity=24.1584158415842, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=27.3743016759777, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=369, Percent_Identity=23.3062330623306, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=532, Percent_Identity=22.5563909774436, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560140, Length=518, Percent_Identity=24.1312741312741, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=539, Percent_Identity=22.6345083487941, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71982997, Length=539, Percent_Identity=22.6345083487941, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=539, Percent_Identity=22.6345083487941, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=540, Percent_Identity=21.8518518518519, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24581924, Length=525, Percent_Identity=23.8095238095238, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922652, Length=497, Percent_Identity=24.3460764587525, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24656500, Length=534, Percent_Identity=24.9063670411985, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=23.1499051233397, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21356947, Length=390, Percent_Identity=24.3589743589744, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18859661, Length=368, Percent_Identity=24.4565217391304, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648260, Length=272, Percent_Identity=26.8382352941176, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667955, Length=564, Percent_Identity=22.6950354609929, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI62472339, Length=540, Percent_Identity=22.5925925925926, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356441, Length=502, Percent_Identity=21.5139442231076, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648253, Length=512, Percent_Identity=22.0703125, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24667959, Length=374, Percent_Identity=23.2620320855615, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648255, Length=500, Percent_Identity=22.6, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20130357, Length=448, Percent_Identity=22.7678571428571, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21358303, Length=499, Percent_Identity=22.4448897795591, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281365686, Length=329, Percent_Identity=25.2279635258359, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 59087; Mature: 58955

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADN
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
HHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGEHNLQDAINHPAEDFTATPSP
CEEEEECCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCCHHHCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALAEGESRAQLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHCCCCEEEEECCHHHHHHHHHHHCCCHHHHHH
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
HHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
PDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEECCCCEEECCCEEEEC
PEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
CCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEE
KEPLRAVQVRRFLREQGIAEYKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRTSA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADN
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
HHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGEHNLQDAINHPAEDFTATPSP
CEEEEECCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCCHHHCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALAEGESRAQLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHCCCCEEEEECCHHHHHHHHHHHCCCHHHHHH
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
HHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
PDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEECCCCEEECCCEEEEC
PEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
CCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEE
KEPLRAVQVRRFLREQGIAEYKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRTSA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]