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The map label for this gene is cof [H]

Identifier: 218688310

GI number: 218688310

Start: 488146

End: 488964

Strand: Direct

Name: cof [H]

Synonym: ECED1_0470

Alternate gene names: 218688310

Gene position: 488146-488964 (Clockwise)

Preceding gene: 218688307

Following gene: 218688311

Centisome position: 9.37

GC content: 50.92

Gene sequence:

>819_bases
ATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCTGACCATCATTTAGGTGAGAAAACCCTCTCTAC
TTTGGCGCGGCTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCG
GGGCGCTATCGCTGGATGCATATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGT
GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA
CGATGGTTGGTTTACCGGGAAAGAGAACCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCG
ATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATC
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTATGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG
CAATAAAGGCGCTGTATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTACGCGATTGCATGGCCTTTGGTGATGCGA
TGAACGATCGCGAAATGTTAGGCAGCGTCGGTAGCGGATTTATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTC
CCGCATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCT
ACCTTATTCCCCCGAATAA

Upstream 100 bases:

>100_bases
TATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGT
GTCACAGAGTGGAGAAAGAA

Downstream 100 bases:

>100_bases
CGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGCTTAAACCG
CACAGAATAAATTGTCGCGA

Product: thiamin pyrimidine pyrophosphate hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGELLHR
DDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKENPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
QLYEALGERAHLCFSATDCLEVLPVGCNKGAVLTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE

Sequences:

>Translated_272_residues
MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGELLHR
DDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKENPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
QLYEALGERAHLCFSATDCLEVLPVGCNKGAVLTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
>Mature_271_residues
ARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRD
DLPADVAELVLYQQWDTRASMHIFNDDGWFTGKENPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQIQ
LYEALGERAHLCFSATDCLEVLPVGCNKGAVLTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAELP
HLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081741, Length=272, Percent_Identity=98.8970588235294, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI48994981, Length=260, Percent_Identity=38.4615384615385, Blast_Score=177, Evalue=5e-46,
Organism=Escherichia coli, GI1786982, Length=286, Percent_Identity=27.6223776223776, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI2367265, Length=244, Percent_Identity=26.2295081967213, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 30358; Mature: 30227

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDA
CCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCE
YLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKENPALL
EEEECCCCEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCHHHH
QAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQIQLYEALGERAHLCFSATDCL
HHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHEEEEEHHHHH
EVLPVGCNKGAVLTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
HHEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCCHHHHHHC
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDA
CCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCE
YLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKENPALL
EEEECCCCEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCHHHH
QAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQIQLYEALGERAHLCFSATDCL
HHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHEEEEEHHHHH
EVLPVGCNKGAVLTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
HHEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCCHHHHHHC
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA