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The map label for this gene is thiI [H]

Identifier: 218688286

GI number: 218688286

Start: 462271

End: 463719

Strand: Direct

Name: thiI [H]

Synonym: ECED1_0446

Alternate gene names: 218688286

Gene position: 462271-463719 (Clockwise)

Preceding gene: 218688281

Following gene: 218688289

Centisome position: 8.87

GC content: 52.24

Gene sequence:

>1449_bases
ATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTAC
CGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCG
CAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCCCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAA
GACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAATATCGCGATCAACTGGAAGGCAAAACCTT
CTGCGTACGCGTGAAGCGCCGCGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATC
AGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGAAGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTGCTG
CTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGTTGTCGCTCATTTCCGGTGG
TTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCG
CGGCGCATGAAATTGGCGTACGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTC
GCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTAT
GATGGTGCGTGCCGCGTCCAAAGTGGCAGAACGTTACGGCGTACAGGCGCTGGTTACCGGTGAAGCGCTCGGCCAGGTGT
CCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGATACGCTGATCCTGCGTCCGCTGATCTCTTACGAC
AAAGAGCACATCATCAACCTGGCTCGCCAGATTGGCACCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGAT
TTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATA
AAGTGGTGGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACC
GTTAATGGCTTCGGCCCGAACGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGA
AGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAGCAAAACCTGGCTGT
TGTGGTGTGAGCGCGGGGTGATGAGCCGCCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTGTAT
CGCCCGTAA

Upstream 100 bases:

>100_bases
TTAGTGGTATACTTCCGCGCCTGGATGCAGCCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTT
GCCAACGAACCATTGCCGCC

Downstream 100 bases:

>100_bases
TATGTGGTTGTAACGTCGCATCTGGTCAGATGCGACGTTTACCGCATCCGACACTACTCGTAATAATTATAAATCCCTGC
CGCCATCACCAGTTGTGATG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVE
DVPFTDMHDIFEKALVQYRDQLEGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFV
AINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYD
KEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTEQEVVEVET
VNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFNNVKVY
RP

Sequences:

>Translated_482_residues
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVE
DVPFTDMHDIFEKALVQYRDQLEGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFV
AINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYD
KEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTEQEVVEVET
VNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFNNVKVY
RP
>Mature_482_residues
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVE
DVPFTDMHDIFEKALVQYRDQLEGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFV
AINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYD
KEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTEQEVVEVET
VNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFNNVKVY
RP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=482, Percent_Identity=99.5850622406639, Blast_Score=984, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 54961; Mature: 54961

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS51165 THUMP ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQR
CCCCEEECCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCE
LAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQLEGKTFCVRVKRRGKHDFS
EHHHHHHHHCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
SIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLLLIKGRYEGIGGFPIGTQEDV
CCCHHHHHCCCHHHHHHHCEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFV
HHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
AINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTN
EEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
LRLIDNVSDTLILRPLISYDKEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAVKSKI
HHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EAEEEKFDFSILDKVVEEANNVDIREIAQQTEQEVVEVETVNGFGPNDVILDIRSIDEQE
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCC
DKPLKVEGIDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFNNVKVY
CCCEEEECCEEEECCHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEE
RP
CC
>Mature Secondary Structure
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQR
CCCCEEECCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCE
LAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQLEGKTFCVRVKRRGKHDFS
EHHHHHHHHCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
SIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLLLIKGRYEGIGGFPIGTQEDV
CCCHHHHHCCCHHHHHHHCEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFV
HHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
AINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTN
EEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
LRLIDNVSDTLILRPLISYDKEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAVKSKI
HHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EAEEEKFDFSILDKVVEEANNVDIREIAQQTEQEVVEVETVNGFGPNDVILDIRSIDEQE
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCC
DKPLKVEGIDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFNNVKVY
CCCEEEECCEEEECCHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEE
RP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA