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The map label for this gene is phoB

Identifier: 218688261

GI number: 218688261

Start: 438128

End: 438817

Strand: Direct

Name: phoB

Synonym: ECED1_0421

Alternate gene names: 218688261

Gene position: 438128-438817 (Clockwise)

Preceding gene: 218688257

Following gene: 218688262

Centisome position: 8.41

GC content: 53.77

Gene sequence:

>690_bases
ATGGCGAGACGTATTCTGGTCGTAGAAGATGAAGCTCCAATTCGCGAAATGGTCTGCTTCGTGCTCGAACAAAATGGCTT
TCAGCCGGTCGAAGCGGAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACT
GGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTG
ATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGTGCGGATGACTATATCACCAAGCCGTT
TTCTCCGAAGGAGCTGGTGGCGCGAATTAAAGCGGTAATGCGCCGTATATCGCCGATGGCGGTGGAAGAGGTGATTGAGA
TGCAGGGGCTAAGTCTCGATCCGACATCTCACCGGGTGATGGCGGGCGAAGAACCGCTGGAGATGGGGCCAACTGAATTT
AAACTGCTGCACTTTTTTATGACGCATCCCGAGCGCGTGTACAGTCGCGAGCAACTGTTAAATCACGTATGGGGAACCAA
CGTGTATGTGGAAGATCGTACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCA
TGGTGCAGACCGTGCGCGGTACAGGGTATCGTTTTTCAACCCGCTTTTAA

Upstream 100 bases:

>100_bases
GAAAGGATGTGCGACGAGCTTTTCATAAATCTGTCATAAATCTGACGCATAATGACGTCGCATTAATGATCGCAACCTAT
TTATTACAACAGGGCAAATC

Downstream 100 bases:

>100_bases
GGCCTTGCTCATCGGACGTAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAA
AAGGCTGGTGCTGGAGCTGC

Product: transcriptional regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVV
MLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF

Sequences:

>Translated_229_residues
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVV
MLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF
>Mature_228_residues
ARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVM
LTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEFK
LLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=100, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=40.1785714285714, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=37.5545851528384, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=36.7713004484305, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=36.6515837104072, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=35.8744394618834, Blast_Score=136, Evalue=9e-34,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=34.0909090909091, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32.8888888888889, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=39.3013100436681, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=34.2222222222222, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=32.7272727272727, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=32.1739130434783, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=27.9661016949153, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=28.6885245901639, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=36.697247706422, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHOB_ECO57 (P0AFJ6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A99685
- PIR:   E85535
- RefSeq:   NP_286137.1
- RefSeq:   NP_308476.1
- ProteinModelPortal:   P0AFJ6
- EnsemblBacteria:   EBESCT00000027202
- EnsemblBacteria:   EBESCT00000059255
- GeneID:   914551
- GeneID:   957349
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0497
- KEGG:   ecs:ECs0449
- GeneTree:   EBGT00050000008651
- HOGENOM:   HBG753323
- OMA:   QDEFTRH
- ProtClustDB:   PRK10161
- BioCyc:   ECOL83334:ECS0449-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00448
- SMART:   SM00862
- TIGRFAMs:   TIGR02154

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 26434; Mature: 26303

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGS
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
GIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCC
>Mature Secondary Structure 
ARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGS
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
GIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796