BacMap

Definition	Escherichia coli ED1a chromosome, complete genome.
Accession	NC_011745
Length	5,209,548

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The map label for this gene is ytbE [H]

Identifier: 218688182

GI number: 218688182

Start: 353155

End: 353931

Strand: Direct

Name: ytbE [H]

Synonym: ECED1_0339

Alternate gene names: 218688182

Gene position: 353155-353931 (Clockwise)

Preceding gene: 218688181

Following gene: 218688186

Centisome position: 6.78

GC content: 43.37

Gene sequence:

>777_bases
GTGTTGAATGCTATCCGCACGGGTTATCGACTCATCGACACTGCAGCGGTATACGGTAATGAAGATGCCGTCGGTGAAGC
CGTTCGTGAAGCCATATCTGAAGGTTTATGTACCCGAGAAGAGTTATTTATAACGTCTAAATTGTGGGTGCAGGATATGT
TGAATCAGGATATTGCAGCAGCAGGTATTGAAGCATCATTAAAAAAATCGGGGCTAGAATACTTCGATCTCTATTTATTG
CACCAGGCCATGCGCGATTATTTTAGTGCGTGGCGTGCACTCGAAGATGCCTATGAAGAGGGCAAATTAAAAGCGATTGG
GGTTTCCAATTTCTACCCTCATGTTCTGGCGAACTTTTGTGAAACGGTAAGAGTTAAACCGATGGTCAACCAGGTCGAGT
TGCATCCCTATTTTGCCCAACCAGAGGCGCTGGCAACCATGAAGTATTATAACGTGCAGCCTGAAGCATGGGCTCCGTTA
GGTGGTGGACGACATAAACCCTTTGAAAATAATCTGCTTCAGAGTATTGCAGATGCCCATCAAAAATCGATTTCTCAGGT
CATTCTGCGTTGGAATATTCAACGGGGAGTGGTCGTTATTCCGAAATCGACACATCAACAGCGTATCGAAGAAAATTTTG
CTATTTGGGATTTCTCACTGACAGAGAAAGAAATGGCTCAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAATCGGTA
AAACATTTTAATCCTGAGTTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGA

Upstream 100 bases:

>100_bases
ATGTTTTCTTCTCGAGCAACTTCTTTAAACAGATGGTGAACCTCGGGATCAGCGATATCAACACCAAAGATCTATTCAAC
TTTCGCTTTCAGAACAGTCA

Downstream 100 bases:

>100_bases
TATTAATCACTTATTTTACCTGAGACGACAAGAATCTTTTAATAGGGGAGTGATATTGACCTTCACTCTGTCATATCTCC
GGTAATATGGCGTCAGGCTT

Product: putative reductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLNAIRTGYRLIDTAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSGLEYFDLYLL
HQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMVNQVELHPYFAQPEALATMKYYNVQPEAWAPL
GGGRHKPFENNLLQSIADAHQKSISQVILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESV
KHFNPEFVRGCLAVKIHD

Sequences:

>Translated_258_residues
MLNAIRTGYRLIDTAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSGLEYFDLYLL
HQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMVNQVELHPYFAQPEALATMKYYNVQPEAWAPL
GGGRHKPFENNLLQSIADAHQKSISQVILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESV
KHFNPEFVRGCLAVKIHD
>Mature_258_residues
MLNAIRTGYRLIDTAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSGLEYFDLYLL
HQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMVNQVELHPYFAQPEALATMKYYNVQPEAWAPL
GGGRHKPFENNLLQSIADAHQKSISQVILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESV
KHFNPEFVRGCLAVKIHD

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI5174695, Length=261, Percent_Identity=39.0804597701149, Blast_Score=171, Evalue=5e-43,
Organism=Homo sapiens, GI223468663, Length=260, Percent_Identity=37.3076923076923, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI24497577, Length=259, Percent_Identity=35.5212355212355, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI5174391, Length=259, Percent_Identity=35.5212355212355, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI310109922, Length=240, Percent_Identity=37.0833333333333, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI24497583, Length=261, Percent_Identity=35.2490421455939, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI310109920, Length=261, Percent_Identity=34.8659003831418, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI24497585, Length=264, Percent_Identity=36.3636363636364, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI5453543, Length=261, Percent_Identity=34.8659003831418, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI45446745, Length=261, Percent_Identity=34.8659003831418, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI4503285, Length=261, Percent_Identity=34.8659003831418, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI300116271, Length=241, Percent_Identity=38.5892116182573, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI300116273, Length=244, Percent_Identity=37.2950819672131, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI291291012, Length=272, Percent_Identity=34.9264705882353, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI93277124, Length=273, Percent_Identity=33.6996336996337, Blast_Score=147, Evalue=9e-36,
Organism=Homo sapiens, GI4502049, Length=260, Percent_Identity=34.2307692307692, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI310109924, Length=150, Percent_Identity=35.3333333333333, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI310109926, Length=78, Percent_Identity=44.8717948717949, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI207028673, Length=78, Percent_Identity=44.8717948717949, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI87082198, Length=234, Percent_Identity=41.025641025641, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1786400, Length=252, Percent_Identity=31.7460317460317, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788081, Length=242, Percent_Identity=31.8181818181818, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI48994888, Length=256, Percent_Identity=26.171875, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17550248, Length=256, Percent_Identity=37.109375, Blast_Score=156, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17537077, Length=255, Percent_Identity=37.2549019607843, Blast_Score=154, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17564128, Length=263, Percent_Identity=35.361216730038, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17537075, Length=258, Percent_Identity=36.4341085271318, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71998625, Length=235, Percent_Identity=34.468085106383, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17566692, Length=253, Percent_Identity=33.201581027668, Blast_Score=136, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17561298, Length=245, Percent_Identity=31.4285714285714, Blast_Score=127, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17538386, Length=243, Percent_Identity=29.2181069958848, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17537079, Length=268, Percent_Identity=29.1044776119403, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17552492, Length=243, Percent_Identity=29.2181069958848, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17562292, Length=247, Percent_Identity=26.7206477732794, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17561300, Length=243, Percent_Identity=28.3950617283951, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321896, Length=274, Percent_Identity=34.6715328467153, Blast_Score=161, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6324694, Length=275, Percent_Identity=31.2727272727273, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320576, Length=262, Percent_Identity=32.4427480916031, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6322556, Length=237, Percent_Identity=34.5991561181435, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319625, Length=284, Percent_Identity=30.9859154929577, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320079, Length=238, Percent_Identity=33.1932773109244, Blast_Score=116, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24662789, Length=264, Percent_Identity=37.8787878787879, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24657054, Length=272, Percent_Identity=37.5, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI21356425, Length=263, Percent_Identity=36.5019011406844, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24663317, Length=270, Percent_Identity=35.1851851851852, Blast_Score=150, Evalue=8e-37,
Organism=Drosophila melanogaster, GI20129731, Length=280, Percent_Identity=33.9285714285714, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24644950, Length=266, Percent_Identity=33.4586466165414, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24662785, Length=259, Percent_Identity=35.5212355212355, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24662781, Length=259, Percent_Identity=35.5212355212355, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI221378297, Length=286, Percent_Identity=31.1188811188811, Blast_Score=137, Evalue=6e-33,
Organism=Drosophila melanogaster, GI45553081, Length=260, Percent_Identity=29.6153846153846, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI281366140, Length=255, Percent_Identity=29.8039215686275, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 29255; Mature: 29255

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNAIRTGYRLIDTAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAA
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHH
AGIEASLKKSGLEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFC
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
ETVRVKPMVNQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAH
HHHCCCCCHHHEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QKSISQVILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESV
HHHHHHHHHHEECCCCEEEECCCHHHHHHHCCCEEEEECCCHHHHHHHHCCCHHHHCCHH
KHFNPEFVRGCLAVKIHD
HHCCHHHHHCEEEEEECC
>Mature Secondary Structure
MLNAIRTGYRLIDTAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAA
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHH
AGIEASLKKSGLEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFC
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
ETVRVKPMVNQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAH
HHHCCCCCHHHEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QKSISQVILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESV
HHHHHHHHHHEECCCCEEEECCCHHHHHHHCCCEEEEECCCHHHHHHHHCCCHHHHCCHH
KHFNPEFVRGCLAVKIHD
HHCCHHHHHCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]