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The map label for this gene is srfJ

Identifier: 218688155

GI number: 218688155

Start: 323372

End: 324715

Strand: Direct

Name: srfJ

Synonym: ECED1_0305

Alternate gene names: NA

Gene position: 323372-324715 (Clockwise)

Preceding gene: 218688154

Following gene: 218688156

Centisome position: 6.21

GC content: 53.2

Gene sequence:

>1344_bases
ATGAAAGGCAGACTCATCTCTTCCGATCCGTATCGTCAGCAATTCCTTGTTGAGCGTGCGGTCTCTTTTTCGCATCGTCA
GCGTGATTGCAGTGAATTAATCAGCGTCTTACCGCGCCACACGTTACAGCAGATTGACGGATTCGGCGGCAGCTTTACCG
AAGGTGCGGGCGTGGTATTCAACAGCATGAGCGAAAAGACGAAGGCGCAATTTCTTTCCCTTTATTTTTCTGATCAGGAA
CATAATTACACTCTGGCGCGGATGCCAATTCAGAGCTGTGATTTTTCCCTGGGCAATTACGCGTATGTCGATTCCAGCGC
TGACCTGCAGCAGGGACGGCTCTCCTTTTCCCGCGATGAAGCGCATTTAATACCGCTGATTTCCGGGGCGTTGCGGTTAA
ATCCACACATGAAGCTGATGGCTTCGCCGTGGAGTCCGCCGGCGTTTATGAAAACTAATAACGATATGAACGGTGGCGGC
AAGCTGCGGCGCGAATGCTACGCCGACTGGGCCGATATCATTATCAACTACCTGCTGGAATACCGCCGCCACGGCATTAA
TGTGCAGGCGCTCTCCGTGCAGAATGAGCCGGTGGCGGTAAAAACCTGGGACTCCTGTCTGTATAGCGTGGAAGAGGAGA
CAGCCTTTGCCGTGCAGTATCTGCGTCCGCGCCTCGCCCGGCAGGGTATGGATGAGATGGAGATCTATATCTGGGATCAC
GATAAAGATGGCCTGGTAGACTGGGCTGAACGCGCCTTTGCTGACGAAGCTAATTATAAGGGAATTAACGGGCTGGCATT
CCACTGGTATACCGGCGACCATTTTTCGCAAATACAGTATCTGGCCCAGTGCCTGCCGGATAAAAAACTCCTGTTTTCCG
AAGGCTGTGTGCCAATGGAGAGCGATGCCGGTAGCCAGATTCGCCACTGGCATACCTATCTCCATGACATGATTGGTAAT
TTCAAATCGGGTTGTAGCGGGTTTATCGACTGGAATCTGCTGTTGAACAGTGAGGGTGGGCCGAATCATCAGGGTAATCT
GTGTGAAGCGCCCATTCAATACGATGCGCAAAACGACGTGCTGCGGCGTAACCACTCCTGGTATGGTATTGGCCACTTCT
GCCGCTATGTGCGTCCGGGGGCGAGGGTCATGCTTTCTTCAAGTTACGATAATCTTCTGGAAGAGGTGGGATTTGTGAAT
CCCGACGGCGAGCGTGTGCTGGTGGTGTATAACCGCGATGTCCAGGAAAGGCGTTGCCGGGTGCTGGATGGCGATAAAGA
GATCGCGTTAACGCTGCCGCCGTCAGGCGCCAGTACGTTGCTATGGCGGCAGGAGTCGATCTGA

Upstream 100 bases:

>100_bases
TAAACCTTCCGCGTATTAAAACCTTCTGCCGGATAGCGATGCTTAAGCGTCCTATCCGGCATATCACAATTTATTATCTT
CTTTTATCCGGAACTTCCCT

Downstream 100 bases:

>100_bases
ATGATGAAGCTGGGATTTAATGAAGCGACCTGTATGCGAAACTCCACGCTGGCACAGGATGTTGTGTTGGCGGAACGTTT
TGGCTATGACTACATTGAAA

Product: putative glucosylceramidase

Products: NA

Alternate protein names: Glucan Endo-1 6-Beta-Glucosidase; Glycoside Hydrolase Family; O-Glycosyl Hydrolase Family; Glycoside Hydrolase Family Protein; O-Glycosyl Hydrolase; Glycosyl Hydrolase; Ricin B Lectin; O-Glycosyl Hydrolase Family Protein; Glycoside Hydrolase; Lysosomal Glucosyl Ceramidase-Like Protein; Glycosy Hydrolase Family Protein; Glucan EndO-1 6-Beta-Glucosidase; Beta-Glycosidase; Helix-Turn-Helix AraC Type; Glycosyl Hydrolase Family; LPXTG-Motif Cell Wall Anchor Domain Protein; Glycosyl Hydrolases Family; Endo-1 6-Beta-D-Glucanase

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKGRLISSDPYRQQFLVERAVSFSHRQRDCSELISVLPRHTLQQIDGFGGSFTEGAGVVFNSMSEKTKAQFLSLYFSDQE
HNYTLARMPIQSCDFSLGNYAYVDSSADLQQGRLSFSRDEAHLIPLISGALRLNPHMKLMASPWSPPAFMKTNNDMNGGG
KLRRECYADWADIIINYLLEYRRHGINVQALSVQNEPVAVKTWDSCLYSVEEETAFAVQYLRPRLARQGMDEMEIYIWDH
DKDGLVDWAERAFADEANYKGINGLAFHWYTGDHFSQIQYLAQCLPDKKLLFSEGCVPMESDAGSQIRHWHTYLHDMIGN
FKSGCSGFIDWNLLLNSEGGPNHQGNLCEAPIQYDAQNDVLRRNHSWYGIGHFCRYVRPGARVMLSSSYDNLLEEVGFVN
PDGERVLVVYNRDVQERRCRVLDGDKEIALTLPPSGASTLLWRQESI

Sequences:

>Translated_447_residues
MKGRLISSDPYRQQFLVERAVSFSHRQRDCSELISVLPRHTLQQIDGFGGSFTEGAGVVFNSMSEKTKAQFLSLYFSDQE
HNYTLARMPIQSCDFSLGNYAYVDSSADLQQGRLSFSRDEAHLIPLISGALRLNPHMKLMASPWSPPAFMKTNNDMNGGG
KLRRECYADWADIIINYLLEYRRHGINVQALSVQNEPVAVKTWDSCLYSVEEETAFAVQYLRPRLARQGMDEMEIYIWDH
DKDGLVDWAERAFADEANYKGINGLAFHWYTGDHFSQIQYLAQCLPDKKLLFSEGCVPMESDAGSQIRHWHTYLHDMIGN
FKSGCSGFIDWNLLLNSEGGPNHQGNLCEAPIQYDAQNDVLRRNHSWYGIGHFCRYVRPGARVMLSSSYDNLLEEVGFVN
PDGERVLVVYNRDVQERRCRVLDGDKEIALTLPPSGASTLLWRQESI
>Mature_447_residues
MKGRLISSDPYRQQFLVERAVSFSHRQRDCSELISVLPRHTLQQIDGFGGSFTEGAGVVFNSMSEKTKAQFLSLYFSDQE
HNYTLARMPIQSCDFSLGNYAYVDSSADLQQGRLSFSRDEAHLIPLISGALRLNPHMKLMASPWSPPAFMKTNNDMNGGG
KLRRECYADWADIIINYLLEYRRHGINVQALSVQNEPVAVKTWDSCLYSVEEETAFAVQYLRPRLARQGMDEMEIYIWDH
DKDGLVDWAERAFADEANYKGINGLAFHWYTGDHFSQIQYLAQCLPDKKLLFSEGCVPMESDAGSQIRHWHTYLHDMIGN
FKSGCSGFIDWNLLLNSEGGPNHQGNLCEAPIQYDAQNDVLRRNHSWYGIGHFCRYVRPGARVMLSSSYDNLLEEVGFVN
PDGERVLVVYNRDVQERRCRVLDGDKEIALTLPPSGASTLLWRQESI

Specific function: Unknown

COG id: COG5520

COG function: function code M; O-Glycosyl hydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI284807150, Length=432, Percent_Identity=30.787037037037, Blast_Score=211, Evalue=2e-54,
Organism=Homo sapiens, GI54607043, Length=432, Percent_Identity=30.787037037037, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI54607045, Length=432, Percent_Identity=30.787037037037, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI54607047, Length=432, Percent_Identity=30.787037037037, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI284807152, Length=382, Percent_Identity=31.151832460733, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17539758, Length=429, Percent_Identity=29.3706293706294, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17539756, Length=437, Percent_Identity=28.8329519450801, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17542884, Length=440, Percent_Identity=27.7272727272727, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25151335, Length=367, Percent_Identity=30.7901907356948, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI115532470, Length=423, Percent_Identity=27.8959810874704, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI193204210, Length=391, Percent_Identity=28.9002557544757, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI115532472, Length=423, Percent_Identity=27.8959810874704, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21355305, Length=456, Percent_Identity=26.0964912280702, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI161078544, Length=443, Percent_Identity=26.410835214447, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 50983; Mature: 50983

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGRLISSDPYRQQFLVERAVSFSHRQRDCSELISVLPRHTLQQIDGFGGSFTEGAGVVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
NSMSEKTKAQFLSLYFSDQEHNYTLARMPIQSCDFSLGNYAYVDSSADLQQGRLSFSRDE
HHHHHHHHHHHHHEEECCCCCCEEEEECCHHHCCCCCCCEEEECCCCCHHHCCCCCCCCC
AHLIPLISGALRLNPHMKLMASPWSPPAFMKTNNDMNGGGKLRRECYADWADIIINYLLE
CEEEHHHHCCEEECCCEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YRRHGINVQALSVQNEPVAVKTWDSCLYSVEEETAFAVQYLRPRLARQGMDEMEIYIWDH
HHHCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
DKDGLVDWAERAFADEANYKGINGLAFHWYTGDHFSQIQYLAQCLPDKKLLFSEGCVPME
CCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHCCCCCCC
SDAGSQIRHWHTYLHDMIGNFKSGCSGFIDWNLLLNSEGGPNHQGNLCEAPIQYDAQNDV
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHH
LRRNHSWYGIGHFCRYVRPGARVMLSSSYDNLLEEVGFVNPDGERVLVVYNRDVQERRCR
HHCCCCEEEHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHE
VLDGDKEIALTLPPSGASTLLWRQESI
EECCCCEEEEEECCCCCCEEEEECCCC
>Mature Secondary Structure
MKGRLISSDPYRQQFLVERAVSFSHRQRDCSELISVLPRHTLQQIDGFGGSFTEGAGVVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
NSMSEKTKAQFLSLYFSDQEHNYTLARMPIQSCDFSLGNYAYVDSSADLQQGRLSFSRDE
HHHHHHHHHHHHHEEECCCCCCEEEEECCHHHCCCCCCCEEEECCCCCHHHCCCCCCCCC
AHLIPLISGALRLNPHMKLMASPWSPPAFMKTNNDMNGGGKLRRECYADWADIIINYLLE
CEEEHHHHCCEEECCCEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YRRHGINVQALSVQNEPVAVKTWDSCLYSVEEETAFAVQYLRPRLARQGMDEMEIYIWDH
HHHCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
DKDGLVDWAERAFADEANYKGINGLAFHWYTGDHFSQIQYLAQCLPDKKLLFSEGCVPME
CCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHCCCCCCC
SDAGSQIRHWHTYLHDMIGNFKSGCSGFIDWNLLLNSEGGPNHQGNLCEAPIQYDAQNDV
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHH
LRRNHSWYGIGHFCRYVRPGARVMLSSSYDNLLEEVGFVNPDGERVLVVYNRDVQERRCR
HHCCCCEEEHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHE
VLDGDKEIALTLPPSGASTLLWRQESI
EECCCCEEEEEECCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA