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The map label for this gene is yadI [H]

Identifier: 218688007

GI number: 218688007

Start: 150469

End: 150909

Strand: Direct

Name: yadI [H]

Synonym: ECED1_0134

Alternate gene names: 218688007

Gene position: 150469-150909 (Clockwise)

Preceding gene: 218688006

Following gene: 218688008

Centisome position: 2.89

GC content: 48.75

Gene sequence:

>441_bases
ATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACT
TCTTCAGTGCCGGGCCGTGAATTTCTGTCGCGGATTAACCTCTAATATGCTCAGCCGCATGATGTGCGATGCCTTGCATG
AAACGGACTCTGGTGAGGGCGTTATCTTTTTAACCGATATAGCCGGAGCGCCACCGTACCGCGTGGCTTCATTATTAAGC
CACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCAT
GACCAGTTCAGCGTTTCGCGAGCATATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAA
ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAA

Upstream 100 bases:

>100_bases
ATTTCCTCTCCTCTGGATTTGGGGGAGAGGAGTTTTGACGGCTATCACCCTTTATCAACAATGGTCAGGGTAGACTGATT
TTCGGCTAAGGAGGAAGGCG

Downstream 100 bases:

>100_bases
CCCGCGATTCTGATGACGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGTACAAACAAGCTGT
TATTCTCCTGCTGATGCTGT

Product: putative PTS Enzyme IIA

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFCRGLTSNMLSRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLS
HKHSRCEVISGVTLPLIEQMMACRETMTSSAFREHIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY

Sequences:

>Translated_146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFCRGLTSNMLSRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLS
HKHSRCEVISGVTLPLIEQMMACRETMTSSAFREHIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY
>Mature_146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFCRGLTSNMLSRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLS
HKHSRCEVISGVTLPLIEQMMACRETMTSSAFREHIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1786321, Length=146, Percent_Identity=96.5753424657534, Blast_Score=295, Evalue=6e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701 [H]

Pfam domain/function: PF03610 EIIA-man [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16424; Mature: 16424

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFCRGLTSNMLSRMMCDALHETDSGEG
CCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMACRETMTSSAFREHIVELG
EEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
APEVSSLWHQQQKNPPFVLKHNLYEY
CCHHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFCRGLTSNMLSRMMCDALHETDSGEG
CCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMACRETMTSSAFREHIVELG
EEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
APEVSSLWHQQQKNPPFVLKHNLYEY
CCHHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503 [H]