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The map label for this gene is lspA

Identifier: 218687901

GI number: 218687901

Start: 26446

End: 26940

Strand: Direct

Name: lspA

Synonym: ECED1_0024

Alternate gene names: 218687901

Gene position: 26446-26940 (Clockwise)

Preceding gene: 218687900

Following gene: 218687902

Centisome position: 0.51

GC content: 52.32

Gene sequence:

>495_bases
ATGAGTCAATCGATCTGTTCAACAGGGCTACGCTGGCTGTGGCTGGTGGTAGTCGTGCTGATTATCGATCTGGGCAGCAA
ATACCTGATCCTCCAGAACTTTGCTCTGGGGGATACGGTCCCGCTGTTCCCGTCGCTTAATCTGCATTATGCGCGTAACT
ATGGCGCAGCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGC
GTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGG
CGCACTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTATGTCGGCGACTGGCACT
TCGCCACCTTCAACCTTGCCGATACTGCCATTTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAA
GCGAAAAAACAATAA

Upstream 100 bases:

>100_bases
ACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAACGTCGCCGGTGACGGT
GAAAAACGTAAGTTTGCCTG

Downstream 100 bases:

>100_bases
TAAACCCTGCCGGATACGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGTCGCATCCGGCAAAA
ATCCTCAAAATATAAGAGCA

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFPSLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGIS
VILAVMMYRSKATQKLNNIAYALIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
AKKQ

Sequences:

>Translated_164_residues
MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFPSLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGIS
VILAVMMYRSKATQKLNNIAYALIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
AKKQ
>Mature_163_residues
SQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFPSLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISV
ILAVMMYRSKATQKLNNIAYALIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSKA
KKQ

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=164, Percent_Identity=99.390243902439, Blast_Score=330, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_ECO24 (A7ZHB6)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461195.1
- STRING:   A7ZHB6
- EnsemblBacteria:   EBESCT00000021639
- GeneID:   5586401
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0027
- eggNOG:   COG0597
- GeneTree:   EBGT00050000010478
- HOGENOM:   HBG724422
- OMA:   ADSSGWQ
- ProtClustDB:   PRK00376
- BioCyc:   ECOL331111:ECE24377A_0027-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 18129; Mature: 17997

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 114-114 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

HASH(0x1b5232ec)-; HASH(0x1e2f01c0)-; HASH(0x1e4738b0)-; HASH(0x95782b4)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFPSLNLHYARNYGAAFSF
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHH
LADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYALIIGGALGNLFDRLWHGF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSKAKKQ
HHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure 
SQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFPSLNLHYARNYGAAFSF
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHH
LADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYALIIGGALGNLFDRLWHGF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSKAKKQ
HHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA