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The map label for this gene is ybaS [H]

Identifier: 218550130

GI number: 218550130

Start: 2885195

End: 2886142

Strand: Direct

Name: ybaS [H]

Synonym: EFER_2818

Alternate gene names: 218550130

Gene position: 2885195-2886142 (Clockwise)

Preceding gene: 218550129

Following gene: 218550131

Centisome position: 62.88

GC content: 50.74

Gene sequence:

>948_bases
ATGGCTACTGAACATGACAAATTACAACAAGCAGTCAAAAAAGCATGGCAACAATATTCAACACTACAAGGCGGCACAAA
CGCCAGCTATATTCCTTATCTTGCCAGTGTTCCGGCAAATCTCTCGGCATTAGCCATTGTTACCGCTGACGGCGACATCA
TTACGCAGGGAGACACCGAATATCGTTTCGCTCTGGAATCCATTTCTAAAATCTGCACCCTTGCACTGGCATTGGAAGAT
GTCGGCCCGGAAGAAGTGCATAACAAGATTGGTGCCGATCCAACTGGCTTACCGTTTAACTCGGTTATTGCTCTGGAACT
GCATCAGGGTAAGCCGCTGTCACCATTGGTCAATGCCGGCGCTATGTCTACCGTCAGCCTGGTGAATGCGCGGGATCGTG
AAAACCGTTGGTCACGCATTCTTCATATGCAGCAGCAACTTACCGGAGCACCAGTGGCACTGTCTGACGAAGTGAATCAA
TCGGAACAAACAACTAATTTCCATAACCGCGCAATTGCCTGGCTGCTGTATTCCGCTAACACACTTTACTGCGATCCCAT
GGAAGCCTGCGACGTTTACACCCGCCAGTGTTCAACGCTTATTAATACCATTGAACTGGCAACCATTGGGGCAACCTTTG
CTGCCGGAGGAGTCAATCCCCTGAGCAAACAACGGGTAGTGAAAGAGGAAAATGTGCCTTTTATTCTGGCGGAGATGATG
ATGGAAGGAATGTATGGCAGCTCTGGCGACTGGGCTTATACCGTTGGATTACCTGCAAAAAGCGGTGTCGGCGGCGGTAT
TCTGGCTGTCGTCCCCGGAGTAATGGGAATTGCGGCCTTTGCCCCGCCCCTGGATGACGCTGGCAATAGCGTTCGTGGGC
AAAAAATGGTGGCAGCCGTTGCTCAGGAACTGGGCTATAACCTGTTTCGTGGTCATCAGACTCGCTGA

Upstream 100 bases:

>100_bases
TAGCCTGAAATAAAACGGTCCTTTCGTACAGGCTATATATTTACATCCAATTATTCACATCCAGATAAAAAGCTGCCTTT
TATTAGAAAGGAGAAAATTA

Downstream 100 bases:

>100_bases
TTAATTCCCGCTTTTTAGGCCAGATGCAAAGATTTAAGAATCATCCCATACCCGTGCCAACTATGCTTAATGTTCAAAAA
ACGAGCGCGGCGCGGATATA

Product: glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MATEHDKLQQAVKKAWQQYSTLQGGTNASYIPYLASVPANLSALAIVTADGDIITQGDTEYRFALESISKICTLALALED
VGPEEVHNKIGADPTGLPFNSVIALELHQGKPLSPLVNAGAMSTVSLVNARDRENRWSRILHMQQQLTGAPVALSDEVNQ
SEQTTNFHNRAIAWLLYSANTLYCDPMEACDVYTRQCSTLINTIELATIGATFAAGGVNPLSKQRVVKEENVPFILAEMM
MEGMYGSSGDWAYTVGLPAKSGVGGGILAVVPGVMGIAAFAPPLDDAGNSVRGQKMVAAVAQELGYNLFRGHQTR

Sequences:

>Translated_315_residues
MATEHDKLQQAVKKAWQQYSTLQGGTNASYIPYLASVPANLSALAIVTADGDIITQGDTEYRFALESISKICTLALALED
VGPEEVHNKIGADPTGLPFNSVIALELHQGKPLSPLVNAGAMSTVSLVNARDRENRWSRILHMQQQLTGAPVALSDEVNQ
SEQTTNFHNRAIAWLLYSANTLYCDPMEACDVYTRQCSTLINTIELATIGATFAAGGVNPLSKQRVVKEENVPFILAEMM
MEGMYGSSGDWAYTVGLPAKSGVGGGILAVVPGVMGIAAFAPPLDDAGNSVRGQKMVAAVAQELGYNLFRGHQTR
>Mature_314_residues
ATEHDKLQQAVKKAWQQYSTLQGGTNASYIPYLASVPANLSALAIVTADGDIITQGDTEYRFALESISKICTLALALEDV
GPEEVHNKIGADPTGLPFNSVIALELHQGKPLSPLVNAGAMSTVSLVNARDRENRWSRILHMQQQLTGAPVALSDEVNQS
EQTTNFHNRAIAWLLYSANTLYCDPMEACDVYTRQCSTLINTIELATIGATFAAGGVNPLSKQRVVKEENVPFILAEMMM
EGMYGSSGDWAYTVGLPAKSGVGGGILAVVPGVMGIAAFAPPLDDAGNSVRGQKMVAAVAQELGYNLFRGHQTR

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutaminase family [H]

Homologues:

Organism=Homo sapiens, GI156104878, Length=296, Percent_Identity=37.1621621621622, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI20336214, Length=295, Percent_Identity=35.5932203389831, Blast_Score=168, Evalue=7e-42,
Organism=Escherichia coli, GI1786693, Length=308, Percent_Identity=76.6233766233766, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1787804, Length=286, Percent_Identity=37.4125874125874, Blast_Score=190, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17507019, Length=314, Percent_Identity=35.3503184713376, Blast_Score=184, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17532727, Length=304, Percent_Identity=32.5657894736842, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI193204073, Length=315, Percent_Identity=32.0634920634921, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI193204075, Length=317, Percent_Identity=31.8611987381703, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI281363241, Length=312, Percent_Identity=33.6538461538462, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI281363239, Length=312, Percent_Identity=33.6538461538462, Blast_Score=170, Evalue=9e-43,
Organism=Drosophila melanogaster, GI24653164, Length=311, Percent_Identity=33.1189710610932, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24653162, Length=312, Percent_Identity=33.6538461538462, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI116008307, Length=312, Percent_Identity=33.6538461538462, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24653156, Length=312, Percent_Identity=33.6538461538462, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24653158, Length=311, Percent_Identity=33.1189710610932, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24653166, Length=312, Percent_Identity=33.6538461538462, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015868 [H]

Pfam domain/function: PF04960 Glutaminase [H]

EC number: =3.5.1.2 [H]

Molecular weight: Translated: 33714; Mature: 33582

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEHDKLQQAVKKAWQQYSTLQGGTNASYIPYLASVPANLSALAIVTADGDIITQGDTE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCEEEEEEEECCCEEECCCCH
YRFALESISKICTLALALEDVGPEEVHNKIGADPTGLPFNSVIALELHQGKPLSPLVNAG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHCCC
AMSTVSLVNARDRENRWSRILHMQQQLTGAPVALSDEVNQSEQTTNFHNRAIAWLLYSAN
CHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHEEEEEEEECC
TLYCDPMEACDVYTRQCSTLINTIELATIGATFAAGGVNPLSKQRVVKEENVPFILAEMM
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHH
MEGMYGSSGDWAYTVGLPAKSGVGGGILAVVPGVMGIAAFAPPLDDAGNSVRGQKMVAAV
HHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AQELGYNLFRGHQTR
HHHHCCHHHCCCCCC
>Mature Secondary Structure 
ATEHDKLQQAVKKAWQQYSTLQGGTNASYIPYLASVPANLSALAIVTADGDIITQGDTE
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCEEEEEEEECCCEEECCCCH
YRFALESISKICTLALALEDVGPEEVHNKIGADPTGLPFNSVIALELHQGKPLSPLVNAG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHCCC
AMSTVSLVNARDRENRWSRILHMQQQLTGAPVALSDEVNQSEQTTNFHNRAIAWLLYSAN
CHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHEEEEEEEECC
TLYCDPMEACDVYTRQCSTLINTIELATIGATFAAGGVNPLSKQRVVKEENVPFILAEMM
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHH
MEGMYGSSGDWAYTVGLPAKSGVGGGILAVVPGVMGIAAFAPPLDDAGNSVRGQKMVAAV
HHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AQELGYNLFRGHQTR
HHHHCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]