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The map label for this gene is dosC [H]

Identifier: 218549979

GI number: 218549979

Start: 2740351

End: 2741769

Strand: Direct

Name: dosC [H]

Synonym: EFER_2664

Alternate gene names: 218549979

Gene position: 2740351-2741769 (Clockwise)

Preceding gene: 218549978

Following gene: 218549980

Centisome position: 59.72

GC content: 43.76

Gene sequence:

>1419_bases
ATGGCAGGTAGCACAATGGAACAGGTTGAATCCGGTAGCGACGATCTTCTTTTTACAGAATGGCAAACACTCATTAATCA
AACACCAGTTGCCGCACATGAACTGCTGTTGTCATTAAGTGACAAAGAGTTACACCATCTGGTAACTATTTTTTATGATT
ACATGTTGTCGCACACTGAAAGTGCCCTGTTCCTGAATACTGAGCAGGTTAACAGCCGCCTTAGTAGCAGCATGTTTCAC
TGGTTACGTGCAGTACTGGGCAGCTCGCAGCAAGATTTACATGAACTGTTAGCAAAGCAGCATGAAATTGGCATCGTTCA
TGCGCGCATCGGTTTACCTATCGATCTGGTCGCTCGGGCTGCACGAAAAATCAAAGAAGAACTTTATCAAATCCTCGCGC
TGCGAAAAGTGGCTGCCGCACATATCATTACCGACGCCATTTGTTTTAGCGGCCTGGCAATTGATACAGCCATAGAAGCA
ATGACGGCATCTTACTCGCCAAGCGTGCAAAATTCAGTCAATGCCGAAGAGCAATACCGCTTGTTGACTATCTATGACGA
TATAAACGTGGAACGGGAAAGACAAATTCGTTCTCTCACCGACTGGGAAAACCAATTTATCTACAATATTGCGACTGGCT
TACCCATAAAAGACCTGGTCTTCTTGTCTAAATCTGAATTTGGCATGTGGTTTGCTCATAAAGGGAAGCATATTCTGGGT
AAAAGCGTGTTGCTTTCCGGGATGGAAACGTTGATGAAAAAGATCGACCACCATCTGAAACAAGAGCTTCCCCTGCACGC
GCAACTGACCACAGACGAACGTCTGAAGTTATTGCAGGAAATTCGCCAGTTAACGGCGCAAATTCATATTTTACTCTCTT
CCATGTTTGAAGATCTGGTAAAAGAAGAAAACGGTAAAGATTCTCTGACACAACTATTAAATCGGCGCTTTATTCCGACG
ATTATGCGGCGGGAAATCTCTATCGCCCTGCACTCACGTAAACCGTTCGCCCTCGCCATGCTGGATATTGATTACTTCAA
GCAAATTAATGATCGTTATGGGCATAATGCCGGGGATAATACGCTGAAAAATGTCTCTGCGATGATTTATGAACATATTC
GCAGCAGTGATTATGTTTTCCGCTATGGTGGCGAAGAATTTATGATTTTGCTTGTCGAAACAGAATTATCACAAGCCAAA
ATTATTCTCGAAAATCTACGTAACAAAATGGCTGGTTTGCGTATCCAGGCTTCAGCCAGTGAAAGTTTTTCGGTCACGGT
CAGTATTGGCGTCGCCGAATATGATTATCATCCTGACTATAAGCTATTGATAGAAAAGGCAGATAGTGCACTTTATTACG
CCAAAAGTCAGGGCCGAAACCGGGTAGAAGTTTTCCAGCCACATATGACAAACGGCTGA

Upstream 100 bases:

>100_bases
GGATACGCCCGTTTAACGTGCAATTCACAATGCGTTCTTCCGGCAAAATCCTCCTTGACCATTGCGCGAAAAATGCCCGT
AATATCACCGAGATTTATTA

Downstream 100 bases:

>100_bases
TTTTTCGTCATTGTCACTCGAAAGCAGTAGATCCGAACCAGGGATGGTATGATAATTCGTCGGATAACTAACGATAAAAT
CGAGCGGGAGGCACATGCTA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAGSTMEQVESGSDDLLFTEWQTLINQTPVAAHELLLSLSDKELHHLVTIFYDYMLSHTESALFLNTEQVNSRLSSSMFH
WLRAVLGSSQQDLHELLAKQHEIGIVHARIGLPIDLVARAARKIKEELYQILALRKVAAAHIITDAICFSGLAIDTAIEA
MTASYSPSVQNSVNAEEQYRLLTIYDDINVERERQIRSLTDWENQFIYNIATGLPIKDLVFLSKSEFGMWFAHKGKHILG
KSVLLSGMETLMKKIDHHLKQELPLHAQLTTDERLKLLQEIRQLTAQIHILLSSMFEDLVKEENGKDSLTQLLNRRFIPT
IMRREISIALHSRKPFALAMLDIDYFKQINDRYGHNAGDNTLKNVSAMIYEHIRSSDYVFRYGGEEFMILLVETELSQAK
IILENLRNKMAGLRIQASASESFSVTVSIGVAEYDYHPDYKLLIEKADSALYYAKSQGRNRVEVFQPHMTNG

Sequences:

>Translated_472_residues
MAGSTMEQVESGSDDLLFTEWQTLINQTPVAAHELLLSLSDKELHHLVTIFYDYMLSHTESALFLNTEQVNSRLSSSMFH
WLRAVLGSSQQDLHELLAKQHEIGIVHARIGLPIDLVARAARKIKEELYQILALRKVAAAHIITDAICFSGLAIDTAIEA
MTASYSPSVQNSVNAEEQYRLLTIYDDINVERERQIRSLTDWENQFIYNIATGLPIKDLVFLSKSEFGMWFAHKGKHILG
KSVLLSGMETLMKKIDHHLKQELPLHAQLTTDERLKLLQEIRQLTAQIHILLSSMFEDLVKEENGKDSLTQLLNRRFIPT
IMRREISIALHSRKPFALAMLDIDYFKQINDRYGHNAGDNTLKNVSAMIYEHIRSSDYVFRYGGEEFMILLVETELSQAK
IILENLRNKMAGLRIQASASESFSVTVSIGVAEYDYHPDYKLLIEKADSALYYAKSQGRNRVEVFQPHMTNG
>Mature_471_residues
AGSTMEQVESGSDDLLFTEWQTLINQTPVAAHELLLSLSDKELHHLVTIFYDYMLSHTESALFLNTEQVNSRLSSSMFHW
LRAVLGSSQQDLHELLAKQHEIGIVHARIGLPIDLVARAARKIKEELYQILALRKVAAAHIITDAICFSGLAIDTAIEAM
TASYSPSVQNSVNAEEQYRLLTIYDDINVERERQIRSLTDWENQFIYNIATGLPIKDLVFLSKSEFGMWFAHKGKHILGK
SVLLSGMETLMKKIDHHLKQELPLHAQLTTDERLKLLQEIRQLTAQIHILLSSMFEDLVKEENGKDSLTQLLNRRFIPTI
MRREISIALHSRKPFALAMLDIDYFKQINDRYGHNAGDNTLKNVSAMIYEHIRSSDYVFRYGGEEFMILLVETELSQAKI
ILENLRNKMAGLRIQASASESFSVTVSIGVAEYDYHPDYKLLIEKADSALYYAKSQGRNRVEVFQPHMTNG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=453, Percent_Identity=36.4238410596026, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI87082007, Length=184, Percent_Identity=33.1521739130435, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87081881, Length=156, Percent_Identity=39.7435897435897, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787262, Length=201, Percent_Identity=32.3383084577114, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786584, Length=160, Percent_Identity=31.875, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=30.9090909090909, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788381, Length=163, Percent_Identity=29.4478527607362, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=33.9285714285714, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=32.7160493827161, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081977, Length=164, Percent_Identity=29.2682926829268, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081974, Length=111, Percent_Identity=32.4324324324324, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 53840; Mature: 53708

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSTMEQVESGSDDLLFTEWQTLINQTPVAAHELLLSLSDKELHHLVTIFYDYMLSHTE
CCCCCHHHHHCCCCCEEHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
SALFLNTEQVNSRLSSSMFHWLRAVLGSSQQDLHELLAKQHEIGIVHARIGLPIDLVARA
CEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHH
ARKIKEELYQILALRKVAAAHIITDAICFSGLAIDTAIEAMTASYSPSVQNSVNAEEQYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
LLTIYDDINVERERQIRSLTDWENQFIYNIATGLPIKDLVFLSKSEFGMWFAHKGKHILG
EEEEEECCCCHHHHHHHHHHHHHHHEEEEHHCCCCHHHHHEECCCCCCEEEECCCCHHHH
KSVLLSGMETLMKKIDHHLKQELPLHAQLTTDERLKLLQEIRQLTAQIHILLSSMFEDLV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEENGKDSLTQLLNRRFIPTIMRREISIALHSRKPFALAMLDIDYFKQINDRYGHNAGDN
HCCCCHHHHHHHHHHCCCHHHHHHHHHEEEECCCCEEEEEECHHHHHHHHHHCCCCCCCH
TLKNVSAMIYEHIRSSDYVFRYGGEEFMILLVETELSQAKIILENLRNKMAGLRIQASAS
HHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHCCEEEECCC
ESFSVTVSIGVAEYDYHPDYKLLIEKADSALYYAKSQGRNRVEVFQPHMTNG
CCEEEEEEEEEEECCCCCCHHHHEECCCCCEEEEHHCCCCCEEEECCCCCCC
>Mature Secondary Structure 
AGSTMEQVESGSDDLLFTEWQTLINQTPVAAHELLLSLSDKELHHLVTIFYDYMLSHTE
CCCCHHHHHCCCCCEEHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
SALFLNTEQVNSRLSSSMFHWLRAVLGSSQQDLHELLAKQHEIGIVHARIGLPIDLVARA
CEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHH
ARKIKEELYQILALRKVAAAHIITDAICFSGLAIDTAIEAMTASYSPSVQNSVNAEEQYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
LLTIYDDINVERERQIRSLTDWENQFIYNIATGLPIKDLVFLSKSEFGMWFAHKGKHILG
EEEEEECCCCHHHHHHHHHHHHHHHEEEEHHCCCCHHHHHEECCCCCCEEEECCCCHHHH
KSVLLSGMETLMKKIDHHLKQELPLHAQLTTDERLKLLQEIRQLTAQIHILLSSMFEDLV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEENGKDSLTQLLNRRFIPTIMRREISIALHSRKPFALAMLDIDYFKQINDRYGHNAGDN
HCCCCHHHHHHHHHHCCCHHHHHHHHHEEEECCCCEEEEEECHHHHHHHHHHCCCCCCCH
TLKNVSAMIYEHIRSSDYVFRYGGEEFMILLVETELSQAKIILENLRNKMAGLRIQASAS
HHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHCCEEEECCC
ESFSVTVSIGVAEYDYHPDYKLLIEKADSALYYAKSQGRNRVEVFQPHMTNG
CCEEEEEEEEEEECCCCCCHHHHEECCCCCEEEEHHCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]