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The map label for this gene is rna [H]

Identifier: 218549802

GI number: 218549802

Start: 2545854

End: 2546666

Strand: Direct

Name: rna [H]

Synonym: EFER_2483

Alternate gene names: 218549802

Gene position: 2545854-2546666 (Clockwise)

Preceding gene: 218549801

Following gene: 218549803

Centisome position: 55.48

GC content: 49.32

Gene sequence:

>813_bases
TTGATCATGAAATTACTCAGATATTCCTGCACATTATTGGCTATCTCACTGCTTCCCTTCACCTCTGCTCGTGCTGACGA
ACTACAGCCTAAGCAGTATGCTGATTTCGATCGCTATGTTCTGGCACTCTCCTGGCAAACAGGCTTTTGTCAAAGCCAGC
ATGACCGTGGGCGCAAAGAACCTGTCGAATGTCGTTTGCAAAAAGAAACCGAAGATAAATCCGCTTTCCTGACTGTTCAC
GGGCTTTGGCCTGGCTTGCCAAAATCAGTAGCGGCACGCGGAGTTGATGAGCGACGCTGGATGCGCTATGGCTGTGCCAC
TCGCCCTATTCCAAATTTTCCTGAAGTACGTGCCAGCCGGAAGTGTTCCTCTCCTGAAACTGGATTATCTCTGGAAACAG
CAGCCAGACTTAGCGAAGTGATGCCCGGAGCAGGCGGGCGTTCATGCCTGGAACGCTATGAATACGCCAAACATGGGGCC
TGTTTTGGCTTCGATCCGGATGCTTACTTCGAGACTATGGTACGCCTGAATAAAGAATTCAAAAGCAGTGCCGTTGGCGA
GTTCCTTGTTGAAAATTACGGTAAAACCGTTTCGCGCAGTGATTTTGACGCTGCTATTGCCAAAAGCTGGGGAAGTGAAA
ACGTCAAAGCGGTAAAATTAAGCTGTCAGGGCAAACCGGCTTATCTGACAGAGATTCAAATCTCCCTGCGCGCCAACCAA
ATCAACGCCCCATTATCTGCCAGTTCCCTCCAACCGCAGCCACACCCGGGGAATTGCGGTAATCAATTTATCATTGATAA
AGTGGGTTATTGA

Upstream 100 bases:

>100_bases
ACCACTGTTGAATAAAGTATCAGGCTCGCCGGGTAAACACGCCCGACGAGCTTTTTTATGGTTACAATAGATAATATTCA
GCATTTGTATATGTTTCGGA

Downstream 100 bases:

>100_bases
CGTGTTGGCAGGGGATTCCAACAAAGCATTCACCTTATGGATGTATGGGATTTGCCTTAATGTGACCTGAATCACTTCAA
AGAGTGTTAAACTCTCAGTA

Product: ribonuclease I

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MIMKLLRYSCTLLAISLLPFTSARADELQPKQYADFDRYVLALSWQTGFCQSQHDRGRKEPVECRLQKETEDKSAFLTVH
GLWPGLPKSVAARGVDERRWMRYGCATRPIPNFPEVRASRKCSSPETGLSLETAARLSEVMPGAGGRSCLERYEYAKHGA
CFGFDPDAYFETMVRLNKEFKSSAVGEFLVENYGKTVSRSDFDAAIAKSWGSENVKAVKLSCQGKPAYLTEIQISLRANQ
INAPLSASSLQPQPHPGNCGNQFIIDKVGY

Sequences:

>Translated_270_residues
MIMKLLRYSCTLLAISLLPFTSARADELQPKQYADFDRYVLALSWQTGFCQSQHDRGRKEPVECRLQKETEDKSAFLTVH
GLWPGLPKSVAARGVDERRWMRYGCATRPIPNFPEVRASRKCSSPETGLSLETAARLSEVMPGAGGRSCLERYEYAKHGA
CFGFDPDAYFETMVRLNKEFKSSAVGEFLVENYGKTVSRSDFDAAIAKSWGSENVKAVKLSCQGKPAYLTEIQISLRANQ
INAPLSASSLQPQPHPGNCGNQFIIDKVGY
>Mature_270_residues
MIMKLLRYSCTLLAISLLPFTSARADELQPKQYADFDRYVLALSWQTGFCQSQHDRGRKEPVECRLQKETEDKSAFLTVH
GLWPGLPKSVAARGVDERRWMRYGCATRPIPNFPEVRASRKCSSPETGLSLETAARLSEVMPGAGGRSCLERYEYAKHGA
CFGFDPDAYFETMVRLNKEFKSSAVGEFLVENYGKTVSRSDFDAAIAKSWGSENVKAVKLSCQGKPAYLTEIQISLRANQ
INAPLSASSLQPQPHPGNCGNQFIIDKVGY

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=268, Percent_Identity=80.2238805970149, Blast_Score=457, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 30092; Mature: 30092

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKLLRYSCTLLAISLLPFTSARADELQPKQYADFDRYVLALSWQTGFCQSQHDRGRKE
CHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHCCCCEEEEEEECCCCCCCCHHHCCCCC
PVECRLQKETEDKSAFLTVHGLWPGLPKSVAARGVDERRWMRYGCATRPIPNFPEVRASR
CHHEEECCCCCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHC
KCSSPETGLSLETAARLSEVMPGAGGRSCLERYEYAKHGACFGFDPDAYFETMVRLNKEF
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KSSAVGEFLVENYGKTVSRSDFDAAIAKSWGSENVKAVKLSCQGKPAYLTEIQISLRANQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEEEEEECC
INAPLSASSLQPQPHPGNCGNQFIIDKVGY
CCCCCCCCCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MIMKLLRYSCTLLAISLLPFTSARADELQPKQYADFDRYVLALSWQTGFCQSQHDRGRKE
CHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHCCCCEEEEEEECCCCCCCCHHHCCCCC
PVECRLQKETEDKSAFLTVHGLWPGLPKSVAARGVDERRWMRYGCATRPIPNFPEVRASR
CHHEEECCCCCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHC
KCSSPETGLSLETAARLSEVMPGAGGRSCLERYEYAKHGACFGFDPDAYFETMVRLNKEF
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KSSAVGEFLVENYGKTVSRSDFDAAIAKSWGSENVKAVKLSCQGKPAYLTEIQISLRANQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEEEEEECC
INAPLSASSLQPQPHPGNCGNQFIIDKVGY
CCCCCCCCCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]