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The map label for this gene is holB [H]

Identifier: 218549174

GI number: 218549174

Start: 1881990

End: 1882994

Strand: Reverse

Name: holB [H]

Synonym: EFER_1828

Alternate gene names: 218549174

Gene position: 1882994-1881990 (Counterclockwise)

Preceding gene: 218549175

Following gene: 218549173

Centisome position: 41.04

GC content: 50.75

Gene sequence:

>1005_bases
ATGAAATGGTATCCATGGTTAAGACCAGACTTCGAAAAACTGGTAGCGAACTATCAGGCAGGAAGGGGTCACCATGCGCT
ACTGATTCAGGCGCTGCCAGGTATGGGCGATGACGCATTAATTTATGCGCTCAGCCGTTACCTGCTTTGCCAACAACCCC
AGGGTAATAAAAGCTGTGGGCACTGTCGTGGCTGCCAACTCATGCAAGCGGGTACGCATCCGGATTACTATGCCCTCATT
CCTGAAAAAGGCAAAAATGCGCAGGGCATTGACGCAGTGCGTGAGGTTAGCGAAAAACTCTATGAGCATTCCCGGCTTGG
GGGGGCAAAAGTGGTCTGGATACCTGATGCCGCTTTGCTCACAGATGCTGCAGCGAATGCACTGTTAAAAACGCTGGAAG
AGCCGCCGGCAGATACCTGGTTTTTCCTTGCCACTAAAGAACCTGCGCGTTTACTGGCAACTTTACGTAGCCGCTGTCTG
GTGCATCATCTGGCACCACCGCCAGAACAGTATGCGATTACCTGGCTTGCCCGGGAAGTGACGGCGTCACAAGAAGTGTT
ACTTGCTGCGTTGCGTTTAAGCTCAGGAGCGCCAGGAGCGGCATTGACATTGCTGCAATCTGAAGCATGGTCGCAAAGAG
ACACCTTATGCCAGGCGTTGTCCGGCAGTGTACAAAATGGTGACTGGTATTCATTGTTACCCAGTCTGAATCATGAACAC
GTTCCTGACAGATTGCACTGGCTGGCGACGTTGTTAACAGATGCTCTAAAATATCAGCATGGCGCGTCTTCTATGACCAA
TGCTGATGCGTATAGTCTGATTGCAACTATCGCACGGCAACTTTCTGCAACGCGAATTCAGGCGATCCTTCGTGACGTCT
GCCAGACTCGCGAACAGTTATTAACGGTAAACAGTTTGAATCGCGAGCTGTTGTTAACCGATCTCCTTCTTCGTATTGAG
AATTACCTGCAACCAGGCACAGTGCTGCCTGTTCCCCATCTTTAA

Upstream 100 bases:

>100_bases
AGGATGCCAGCATCCGCACTATCGATGCCACGCAATCGCTTGAGTGTGTCATGGCTGATATTCGACAAACTGTGACAACC
TGGGTGAAGGAGCAGGGCGC

Downstream 100 bases:

>100_bases
GCGAGATATTATGTTTTTAGTTGACTCACACTGTCATCTTGATGGTCTGGATTATCAATCCTTGCATAAGGACGTGGATG
ACGTGCTGGCAAAAGCCGCT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKWYPWLRPDFEKLVANYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGNKSCGHCRGCQLMQAGTHPDYYALI
PEKGKNAQGIDAVREVSEKLYEHSRLGGAKVVWIPDAALLTDAAANALLKTLEEPPADTWFFLATKEPARLLATLRSRCL
VHHLAPPPEQYAITWLAREVTASQEVLLAALRLSSGAPGAALTLLQSEAWSQRDTLCQALSGSVQNGDWYSLLPSLNHEH
VPDRLHWLATLLTDALKYQHGASSMTNADAYSLIATIARQLSATRIQAILRDVCQTREQLLTVNSLNRELLLTDLLLRIE
NYLQPGTVLPVPHL

Sequences:

>Translated_334_residues
MKWYPWLRPDFEKLVANYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGNKSCGHCRGCQLMQAGTHPDYYALI
PEKGKNAQGIDAVREVSEKLYEHSRLGGAKVVWIPDAALLTDAAANALLKTLEEPPADTWFFLATKEPARLLATLRSRCL
VHHLAPPPEQYAITWLAREVTASQEVLLAALRLSSGAPGAALTLLQSEAWSQRDTLCQALSGSVQNGDWYSLLPSLNHEH
VPDRLHWLATLLTDALKYQHGASSMTNADAYSLIATIARQLSATRIQAILRDVCQTREQLLTVNSLNRELLLTDLLLRIE
NYLQPGTVLPVPHL
>Mature_334_residues
MKWYPWLRPDFEKLVANYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGNKSCGHCRGCQLMQAGTHPDYYALI
PEKGKNAQGIDAVREVSEKLYEHSRLGGAKVVWIPDAALLTDAAANALLKTLEEPPADTWFFLATKEPARLLATLRSRCL
VHHLAPPPEQYAITWLAREVTASQEVLLAALRLSSGAPGAALTLLQSEAWSQRDTLCQALSGSVQNGDWYSLLPSLNHEH
VPDRLHWLATLLTDALKYQHGASSMTNADAYSLIATIARQLSATRIQAILRDVCQTREQLLTVNSLNRELLLTDLLLRIE
NYLQPGTVLPVPHL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=334, Percent_Identity=78.4431137724551, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1786676, Length=144, Percent_Identity=34.0277777777778, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36990; Mature: 36990

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWYPWLRPDFEKLVANYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGNKSCG
CCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCC
HCRGCQLMQAGTHPDYYALIPEKGKNAQGIDAVREVSEKLYEHSRLGGAKVVWIPDAALL
CCCCCHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
TDAAANALLKTLEEPPADTWFFLATKEPARLLATLRSRCLVHHLAPPPEQYAITWLAREV
HHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TASQEVLLAALRLSSGAPGAALTLLQSEAWSQRDTLCQALSGSVQNGDWYSLLPSLNHEH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCC
VPDRLHWLATLLTDALKYQHGASSMTNADAYSLIATIARQLSATRIQAILRDVCQTREQL
CHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVNSLNRELLLTDLLLRIENYLQPGTVLPVPHL
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKWYPWLRPDFEKLVANYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGNKSCG
CCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCC
HCRGCQLMQAGTHPDYYALIPEKGKNAQGIDAVREVSEKLYEHSRLGGAKVVWIPDAALL
CCCCCHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
TDAAANALLKTLEEPPADTWFFLATKEPARLLATLRSRCLVHHLAPPPEQYAITWLAREV
HHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TASQEVLLAALRLSSGAPGAALTLLQSEAWSQRDTLCQALSGSVQNGDWYSLLPSLNHEH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCC
VPDRLHWLATLLTDALKYQHGASSMTNADAYSLIATIARQLSATRIQAILRDVCQTREQL
CHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVNSLNRELLLTDLLLRIENYLQPGTVLPVPHL
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 8509334; 8905232; 9278503; 8505304; 1575709; 9363942 [H]