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The map label for this gene is phoQ [H]

Identifier: 218549148

GI number: 218549148

Start: 1855562

End: 1857022

Strand: Direct

Name: phoQ [H]

Synonym: EFER_1801

Alternate gene names: 218549148

Gene position: 1855562-1857022 (Clockwise)

Preceding gene: 218549147

Following gene: 218549149

Centisome position: 40.44

GC content: 47.78

Gene sequence:

>1461_bases
ATGAAAAAACTATTGCGTCATATTTTTCCCCTGTCGTTACGGCTGCGTTTTTTGCTGGCAACGGCAGCGGTTGTTCTGGT
GCTTTCACTGGCTTATGGCATGGTGGCACTTATTGGCTATAGCGTCAGTTTCGATAAAACGACCTTCCGCCTGTTGCGCG
GTGAAAGTAATCTTTTCTATACGCTTGCCAAATGGGACGACAACCAGTTGCATGTTGAGTTGCCGGAGAATATTGACCGG
CAAAGTCCAACGATGTCGATCATTTATGATGAAAATGGCAAATTGCTTTGGGCGCAACGCAATGTACCGTGGTTAATTAA
ACAGATTCGACCGGAGTGGTTAAAAACAAACGGTTTTCATGAAATCGAAACGGATGTTAATTCCACCAGCAGTTTACTGA
CTAAAGATCACAGCGTTCAGGAGCAGCTTAATGAAGTACGGGAAGATGATAGTGACGCCGAAATGACGCATTCGGTGGCG
GTAAATGTGTATCCGGCAACCGCGCAAATGCCGCAGTTGACGATTGTGGTGGTGGACACTGTTCCCGTAGAACTTAAACG
TTCTTATATGGTGTGGAGCTGGTTTGTCTATGTGTTGATAGCAAACCTGTTACTGGTAATTCCTCTTCTTTGGGTCGCCG
CATGGTGGAGTTTGCGTCCAATTGAAGCGCTGGCCCGTGAGGTGCATGAACTGGAAGAGCATCACCGCGAAATGCTCAAT
CCCGATACCACCCGTGAGCTCACCAGTCTGGTGCGCAATCTTAACCGCCTGTTAAAAAGCGAACGTGAACGTTATGACAA
ATACCGCACCACGCTCACAGACCTTACCCATAGCCTGAAAACGCCTCTCGCTGTTTTACAAAGCACCTTGCGCTCTCTGC
GTAACGGAAAAATGACCGTCAATGATGCTGAACCCATAATGCTGGAACAAATCAGCCGTATTTCGCAGCAAATTGGTTAT
TATTTACACCGAGCGAATATGCGCGGCGGCGGATTACTCAGCCGGGAGCTACATCCTGTCGCGCCGCTGTTGGATAATCT
CATTTGTGCATTAAATAAGGTTTATCAGCGTAAAGGGGTGACAATAACTGTCGATATTTCCCCAGAAATCAGTTTTGTGG
GCGAGAAAAATGATTTTGTCGAAGTCATGGGAAATGTGCTCGATAACGCCTGCAAATATTGTCTTGAGTTTGTTGAGATT
TCCGCCCGCCTGACTGATGATGCGTTGCATATCATTGTGGAAGATGATGGCCCTGGTATCCCACCTGAGAAACGAGAAAT
GGTGTTTGATCGCGGGCAACGAGTCGATACCCTTCGTCCTGGTCAGGGGGTTGGACTGGCTGTGGCACGCGAGATCACCG
AACAGTATGATGGGCAAATCATAGCCGGAGAAAGCCTACCTGGCGGTGCCAGAATGGAGGTCATTTTTGGACGTCAGCAA
GCAGGTCAGAAAAATGATTAA

Upstream 100 bases:

>100_bases
ATGTATTAATGGGGCGACTACGTAAAAAAATTCAGGCCCAGTATCCGGATGAGGTCATTACCACTGTGCGTGGTCAGGGA
TATTTATTCGAACTTCACTG

Downstream 100 bases:

>100_bases
CCTGCATGTAATAAATATGAGCAGATTGCCCTAATAAGGTTAAGCATCCGTTATAATCGCTGACAGATATCAGTCTGTGG
ATGCTCATTATGGAATACCA

Product: sensor protein PhoQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKKLLRHIFPLSLRLRFLLATAAVVLVLSLAYGMVALIGYSVSFDKTTFRLLRGESNLFYTLAKWDDNQLHVELPENIDR
QSPTMSIIYDENGKLLWAQRNVPWLIKQIRPEWLKTNGFHEIETDVNSTSSLLTKDHSVQEQLNEVREDDSDAEMTHSVA
VNVYPATAQMPQLTIVVVDTVPVELKRSYMVWSWFVYVLIANLLLVIPLLWVAAWWSLRPIEALAREVHELEEHHREMLN
PDTTRELTSLVRNLNRLLKSERERYDKYRTTLTDLTHSLKTPLAVLQSTLRSLRNGKMTVNDAEPIMLEQISRISQQIGY
YLHRANMRGGGLLSRELHPVAPLLDNLICALNKVYQRKGVTITVDISPEISFVGEKNDFVEVMGNVLDNACKYCLEFVEI
SARLTDDALHIIVEDDGPGIPPEKREMVFDRGQRVDTLRPGQGVGLAVAREITEQYDGQIIAGESLPGGARMEVIFGRQQ
AGQKND

Sequences:

>Translated_486_residues
MKKLLRHIFPLSLRLRFLLATAAVVLVLSLAYGMVALIGYSVSFDKTTFRLLRGESNLFYTLAKWDDNQLHVELPENIDR
QSPTMSIIYDENGKLLWAQRNVPWLIKQIRPEWLKTNGFHEIETDVNSTSSLLTKDHSVQEQLNEVREDDSDAEMTHSVA
VNVYPATAQMPQLTIVVVDTVPVELKRSYMVWSWFVYVLIANLLLVIPLLWVAAWWSLRPIEALAREVHELEEHHREMLN
PDTTRELTSLVRNLNRLLKSERERYDKYRTTLTDLTHSLKTPLAVLQSTLRSLRNGKMTVNDAEPIMLEQISRISQQIGY
YLHRANMRGGGLLSRELHPVAPLLDNLICALNKVYQRKGVTITVDISPEISFVGEKNDFVEVMGNVLDNACKYCLEFVEI
SARLTDDALHIIVEDDGPGIPPEKREMVFDRGQRVDTLRPGQGVGLAVAREITEQYDGQIIAGESLPGGARMEVIFGRQQ
AGQKND
>Mature_486_residues
MKKLLRHIFPLSLRLRFLLATAAVVLVLSLAYGMVALIGYSVSFDKTTFRLLRGESNLFYTLAKWDDNQLHVELPENIDR
QSPTMSIIYDENGKLLWAQRNVPWLIKQIRPEWLKTNGFHEIETDVNSTSSLLTKDHSVQEQLNEVREDDSDAEMTHSVA
VNVYPATAQMPQLTIVVVDTVPVELKRSYMVWSWFVYVLIANLLLVIPLLWVAAWWSLRPIEALAREVHELEEHHREMLN
PDTTRELTSLVRNLNRLLKSERERYDKYRTTLTDLTHSLKTPLAVLQSTLRSLRNGKMTVNDAEPIMLEQISRISQQIGY
YLHRANMRGGGLLSRELHPVAPLLDNLICALNKVYQRKGVTITVDISPEISFVGEKNDFVEVMGNVLDNACKYCLEFVEI
SARLTDDALHIIVEDDGPGIPPEKREMVFDRGQRVDTLRPGQGVGLAVAREITEQYDGQIIAGESLPGGARMEVIFGRQQ
AGQKND

Specific function: Member of the two-component regulatory system phoQ/phoP involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane- associated p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=486, Percent_Identity=85.59670781893, Blast_Score=873, Evalue=0.0,
Organism=Escherichia coli, GI1788393, Length=272, Percent_Identity=26.1029411764706, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1790346, Length=260, Percent_Identity=27.3076923076923, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787894, Length=273, Percent_Identity=27.4725274725275, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786783, Length=275, Percent_Identity=23.6363636363636, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786912, Length=273, Percent_Identity=28.9377289377289, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789808, Length=228, Percent_Identity=26.7543859649123, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790551, Length=280, Percent_Identity=26.7857142857143, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786600, Length=257, Percent_Identity=22.568093385214, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55350; Mature: 55350

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLRHIFPLSLRLRFLLATAAVVLVLSLAYGMVALIGYSVSFDKTTFRLLRGESNLFY
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEE
TLAKWDDNQLHVELPENIDRQSPTMSIIYDENGKLLWAQRNVPWLIKQIRPEWLKTNGFH
EEEECCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHCHHHHHCCCCE
EIETDVNSTSSLLTKDHSVQEQLNEVREDDSDAEMTHSVAVNVYPATAQMPQLTIVVVDT
EECCCCCCHHHHHHCCHHHHHHHHHHHCCCCCHHHHHEEEEEEEECCCCCCEEEEEEEEC
VPVELKRSYMVWSWFVYVLIANLLLVIPLLWVAAWWSLRPIEALAREVHELEEHHREMLN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
PDTTRELTSLVRNLNRLLKSERERYDKYRTTLTDLTHSLKTPLAVLQSTLRSLRNGKMTV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
NDAEPIMLEQISRISQQIGYYLHRANMRGGGLLSRELHPVAPLLDNLICALNKVYQRKGV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
TITVDISPEISFVGEKNDFVEVMGNVLDNACKYCLEFVEISARLTDDALHIIVEDDGPGI
EEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
PPEKREMVFDRGQRVDTLRPGQGVGLAVAREITEQYDGQIIAGESLPGGARMEVIFGRQQ
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEECCCC
AGQKND
CCCCCC
>Mature Secondary Structure
MKKLLRHIFPLSLRLRFLLATAAVVLVLSLAYGMVALIGYSVSFDKTTFRLLRGESNLFY
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEE
TLAKWDDNQLHVELPENIDRQSPTMSIIYDENGKLLWAQRNVPWLIKQIRPEWLKTNGFH
EEEECCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHCHHHHHCCCCE
EIETDVNSTSSLLTKDHSVQEQLNEVREDDSDAEMTHSVAVNVYPATAQMPQLTIVVVDT
EECCCCCCHHHHHHCCHHHHHHHHHHHCCCCCHHHHHEEEEEEEECCCCCCEEEEEEEEC
VPVELKRSYMVWSWFVYVLIANLLLVIPLLWVAAWWSLRPIEALAREVHELEEHHREMLN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
PDTTRELTSLVRNLNRLLKSERERYDKYRTTLTDLTHSLKTPLAVLQSTLRSLRNGKMTV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
NDAEPIMLEQISRISQQIGYYLHRANMRGGGLLSRELHPVAPLLDNLICALNKVYQRKGV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
TITVDISPEISFVGEKNDFVEVMGNVLDNACKYCLEFVEISARLTDDALHIIVEDDGPGI
EEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
PPEKREMVFDRGQRVDTLRPGQGVGLAVAREITEQYDGQIIAGESLPGGARMEVIFGRQQ
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEECCCC
AGQKND
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]