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The map label for this gene is nhaB

Identifier: 218549117

GI number: 218549117

Start: 1820310

End: 1821854

Strand: Direct

Name: nhaB

Synonym: EFER_1769

Alternate gene names: 218549117

Gene position: 1820310-1821854 (Clockwise)

Preceding gene: 218549115

Following gene: 218549118

Centisome position: 39.67

GC content: 48.35

Gene sequence:

>1545_bases
ATGGAAATCTCCTGGGGCCGCGCCTTATGGCGTAACTTTCTGGGTCAGTCACCAGACTGGTACAAACTTGCACTTTTGAT
CTTCTTAATCATTAATCCGCTGATTTTTTTAGTTAATCCATTCATAGCTGGTTGGTTATTAGTCGCCGAATTTATATTTA
CCCTCGCAATGGCGCTTAAATGTTATCCACTCTTACCCGGTGGATTGCTGGCAATTGAAGCGGTGATGATCGGCATGACC
AGTCCATCACACGTACGTGCAGAGGTTGCGGCGAACCTTGAAGTATTACTGCTACTTATGTTTATGGTTGCAGGTATTTA
CTTTATGAAGCAATTGCTGCTGTTTATCTTCACCCGGTTGCTGCTCAGTATCCGTTCTAAGGCTTTACTCTCACTCTCTT
TCTGCCTTGCTGCAGCCTTCCTTTCTGCCTTCCTTGACGCCCTGACCGTCGTGGCAGTCGTAATAAGTGTTGCCGTGGGC
TTTTATGGTATCTATCACCGGGTGGCTTCTTCTCGTGGCGAAGATAACGATATTCTCGATGACAGCCATATTGATCAGCA
CTTTAAAGTTATTCTGGAGCAATTCCGTGGATTTCTGCGCAGCCTGATGATGCATGCTGGTGTGGGCACCGCGCTTGGCG
GGGTAATGACCATGGTTGGTGAACCGCAGAACTTGATTATTGCCAAAGCCGCAGGCTGGAGTTTTGGCGACTTCTTCCTG
CGTATGTCTCCCATTACTGTACCAGTGCTGGTTTGTGGTCTGTTGACTTGCTTGCTGGTAGAGAGGATGGGCTGGTTTGG
CTATGGCGAGAAGCTGCCGGAGAAAGTGCGTCAGGTGCTGCAACAGTACGACGATCAAAGCCGCCTTCAGCGTACCCGTC
AGGATAAAGTTCGCCTGATTGTCCAGGCACTGATTGGCATCTGGTTGGTTATCGCCCTCGCCCTGCATCTGGCTGAAGTT
GGTTTAATTGGTTTGTCAGTAATTATTATGGCGACATCACTGACAGGGGTAACCGACGAACACGCTATCGGTAAAGCCTT
CACTGAGTCACTACCGTTTACCGCACTGTTAACCGTCTTTTTCTCGATTGTCGCGGTGATCATTGATCAGAGTTTGTTCT
CACCGATTATTCATTTTGTATTGCAGGCGTCGGAACATGCGCAGTTAACATTGTTCTATCTGTTTAATGGTCTGCTTTCT
TCTATTTCCGATAACGTCTTCGTCGGTACGATCTATATCAACGAAGCTAAAGCGGCGATGGAAAACGGCACTATCTCCCT
TAATCAGTTTGAACTGTTGGCAGCAGCAATTAATACCGGAACCAACCTGCCTTCAGTCGCCACACCAAATGGTCAGGCGG
CCTTCTTGTTCCTGCTGACTTCCGCCCTTGCACCGCTGATTCGTCTCTCTTATGGTCGAATGGTATGGATGGCGCTGCCT
TATACCATTGTCCTGACCTGTGTCGGTTTGCTTTGCGTGGAATTTACGCTGGCCCCCATGACAGAATGGATGACTCAACA
AGGCTGGCTAGCTACACTTTCGTAA

Upstream 100 bases:

>100_bases
GCCTGCTGTAGTGAAGTTCTTGCCTGATTTCGTCACGATCGTTAAGCTTAGTTCGCTAAGATAAAAACACCATTTCATTT
TTATAGGTAAGGGAAACCAC

Downstream 100 bases:

>100_bases
CAACATACCGGGCTAATCACTGCCCGGTTTATTTTTTCGCCGATTAATTACCTAATTACACTTTATTTTATTTCGCGCTA
GTGGCGTGACAAATGAATTG

Product: sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaB

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MEISWGRALWRNFLGQSPDWYKLALLIFLIINPLIFLVNPFIAGWLLVAEFIFTLAMALKCYPLLPGGLLAIEAVMIGMT
SPSHVRAEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRLLLSIRSKALLSLSFCLAAAFLSAFLDALTVVAVVISVAVG
FYGIYHRVASSRGEDNDILDDSHIDQHFKVILEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWSFGDFFL
RMSPITVPVLVCGLLTCLLVERMGWFGYGEKLPEKVRQVLQQYDDQSRLQRTRQDKVRLIVQALIGIWLVIALALHLAEV
GLIGLSVIIMATSLTGVTDEHAIGKAFTESLPFTALLTVFFSIVAVIIDQSLFSPIIHFVLQASEHAQLTLFYLFNGLLS
SISDNVFVGTIYINEAKAAMENGTISLNQFELLAAAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
YTIVLTCVGLLCVEFTLAPMTEWMTQQGWLATLS

Sequences:

>Translated_514_residues
MEISWGRALWRNFLGQSPDWYKLALLIFLIINPLIFLVNPFIAGWLLVAEFIFTLAMALKCYPLLPGGLLAIEAVMIGMT
SPSHVRAEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRLLLSIRSKALLSLSFCLAAAFLSAFLDALTVVAVVISVAVG
FYGIYHRVASSRGEDNDILDDSHIDQHFKVILEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWSFGDFFL
RMSPITVPVLVCGLLTCLLVERMGWFGYGEKLPEKVRQVLQQYDDQSRLQRTRQDKVRLIVQALIGIWLVIALALHLAEV
GLIGLSVIIMATSLTGVTDEHAIGKAFTESLPFTALLTVFFSIVAVIIDQSLFSPIIHFVLQASEHAQLTLFYLFNGLLS
SISDNVFVGTIYINEAKAAMENGTISLNQFELLAAAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
YTIVLTCVGLLCVEFTLAPMTEWMTQQGWLATLS
>Mature_514_residues
MEISWGRALWRNFLGQSPDWYKLALLIFLIINPLIFLVNPFIAGWLLVAEFIFTLAMALKCYPLLPGGLLAIEAVMIGMT
SPSHVRAEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRLLLSIRSKALLSLSFCLAAAFLSAFLDALTVVAVVISVAVG
FYGIYHRVASSRGEDNDILDDSHIDQHFKVILEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWSFGDFFL
RMSPITVPVLVCGLLTCLLVERMGWFGYGEKLPEKVRQVLQQYDDQSRLQRTRQDKVRLIVQALIGIWLVIALALHLAEV
GLIGLSVIIMATSLTGVTDEHAIGKAFTESLPFTALLTVFFSIVAVIIDQSLFSPIIHFVLQASEHAQLTLFYLFNGLLS
SISDNVFVGTIYINEAKAAMENGTISLNQFELLAAAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
YTIVLTCVGLLCVEFTLAPMTEWMTQQGWLATLS

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3067

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaB Na(+)/H(+) (TC 2.A.34) antiporter family

Homologues:

Organism=Escherichia coli, GI1787435, Length=513, Percent_Identity=90.0584795321637, Blast_Score=912, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAB_ESCF3 (B7LSJ8)

Other databases:

- EMBL:   CU928158
- RefSeq:   YP_002382908.1
- EnsemblBacteria:   EBESCT00000122626
- GeneID:   7119989
- GenomeReviews:   CU928158_GR
- KEGG:   efe:EFER_1769
- GeneTree:   EBGT00050000011272
- HOGENOM:   HBG296776
- ProtClustDB:   PRK09547
- BioCyc:   EFER585054:EFER_1769-MONOMER
- HAMAP:   MF_01599
- InterPro:   IPR004671
- TIGRFAMs:   TIGR00774

Pfam domain/function: PF06450 NhaB

EC number: NA

Molecular weight: Translated: 56572; Mature: 56572

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x295b0cdc)-; HASH(0x29237390)-; HASH(0x278b6628)-; HASH(0x2997f66c)-; HASH(0x29854e80)-; HASH(0x298b7a6c)-; HASH(0x27ebc2a4)-; HASH(0x29404984)-; HASH(0x297b0ed4)-; HASH(0x29520764)-; HASH(0x1f64e4f8)-; HASH(0x265b6fc8)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISWGRALWRNFLGQSPDWYKLALLIFLIINPLIFLVNPFIAGWLLVAEFIFTLAMALK
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CYPLLPGGLLAIEAVMIGMTSPSHVRAEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRL
HHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSIRSKALLSLSFCLAAAFLSAFLDALTVVAVVISVAVGFYGIYHRVASSRGEDNDILD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DSHIDQHFKVILEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWSFGDFFL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
RMSPITVPVLVCGLLTCLLVERMGWFGYGEKLPEKVRQVLQQYDDQSRLQRTRQDKVRLI
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VQALIGIWLVIALALHLAEVGLIGLSVIIMATSLTGVTDEHAIGKAFTESLPFTALLTVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHH
FSIVAVIIDQSLFSPIIHFVLQASEHAQLTLFYLFNGLLSSISDNVFVGTIYINEAKAAM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECHHHHH
ENGTISLNQFELLAAAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
CCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTIVLTCVGLLCVEFTLAPMTEWMTQQGWLATLS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECH
>Mature Secondary Structure
MEISWGRALWRNFLGQSPDWYKLALLIFLIINPLIFLVNPFIAGWLLVAEFIFTLAMALK
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CYPLLPGGLLAIEAVMIGMTSPSHVRAEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRL
HHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSIRSKALLSLSFCLAAAFLSAFLDALTVVAVVISVAVGFYGIYHRVASSRGEDNDILD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DSHIDQHFKVILEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWSFGDFFL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
RMSPITVPVLVCGLLTCLLVERMGWFGYGEKLPEKVRQVLQQYDDQSRLQRTRQDKVRLI
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VQALIGIWLVIALALHLAEVGLIGLSVIIMATSLTGVTDEHAIGKAFTESLPFTALLTVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHH
FSIVAVIIDQSLFSPIIHFVLQASEHAQLTLFYLFNGLLSSISDNVFVGTIYINEAKAAM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECHHHHH
ENGTISLNQFELLAAAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
CCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTIVLTCVGLLCVEFTLAPMTEWMTQQGWLATLS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3 Proton [Periplasm]; Na (I) [Cytoplasm] [C]

Specific reaction: 3 Proton [Periplasm] + 2 Na (I) [Cytoplasm] = 2 Na (I) [Periplasm] + 3 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA