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The map label for this gene is dadX [H]

Identifier: 218549113

GI number: 218549113

Start: 1815068

End: 1816138

Strand: Reverse

Name: dadX [H]

Synonym: EFER_1765

Alternate gene names: 218549113

Gene position: 1816138-1815068 (Counterclockwise)

Preceding gene: 218549114

Following gene: 218549110

Centisome position: 39.58

GC content: 52.57

Gene sequence:

>1071_bases
ATGACGCGACCTATACAGGCCAGTGTTGATTTACAGGCGTTGAAACAAAATCTGGCGATTGTCCGCCGGGCTGCGCCTGA
TGCGCGCGTCTGGTCAGTGGTAAAAGCCAATGCCTATGGACATGGGATCGAACGTATATGGAATGCACTTGCCTCGACAG
ATGGCTTTGCATTACTCAACATTGAAGATGCGGTGACACTGCGCGAACGCGGCTGGAAAGGGCCCATTCTGATGCTTGAG
GGTTTTTTCCATGCTGATGATTTAGCAGTCTATGACAAGTATCGTCTCACAACGAGTGTTCACAGCAACTGGCAGCTTAA
AGCATTGCAGAATGCCCGTCTGAATGCCCCGCTTGATATCTATGTGAAAGTCAACAGTGGCATGAACCGCCTGGGATTTC
CTCCCGAAAAGATACCCACTGTCTGGCAACAACTGCGGGGGATGAGCAATGTCGGAGAAATGACGCTGATGTCGCATTTT
GCTGATGCCGAACATCCTGAAGGTATTGTTCAGGCACTGGAAAAGGTGGAACAAGCCAGTGAAGGGATGGAGTGCCGTCG
TTCGCTGGCTAACTCCGCGGCAACACTTTGGCATCCGCAAGCGCATTTTGACTGGGTACGTCCGGGGATCATTTTGTATG
GTGCTTCGCCTACGGGACACTGGTGTGATATCGCCAATACCGGGCTTAAACCTGTTATGACGCTCAGCAGTGAAATTATC
GGAGTTCAGACATTAAAAGCTGGAGATCGTGTTGGCTATGGCGGGCGTTACACCGCCAGCGGTGAGCAACGGATCGGTAT
TGTTGCTGCGGGCTATGCCGATGGTTACCCCCGTCATGCCCCAACGGGTACGCCGGTACTGGTGGATGGTGTGCGAACCA
TTACCGTTGGCACCGTTTCAATGGATATGCTGGCGGTGGATTTAACACCTTGTCCGCAGGCAGGAATAGGTACGCCAGTA
GAACTGTGGGGTAAGGAGATCAAAATTGATGACGTGGCAGCCGCGGCGGGTACAGTGGGCTATGAGTTGATGTGTGCTCT
GGCACTGCGTGTGCCAGTAGTGACAGTGTAA

Upstream 100 bases:

>100_bases
CTATTCCTTACGATGATCTGAGCGTGGCGCGTTACAGCAGTGGTTTTAGCCCGGCAAGACCGCGTCATTTGCACGGTGTT
CATAACTAAAAGGAAACGAA

Downstream 100 bases:

>100_bases
CGCATTCATACCGGAGAAGACAGTCGTCTCTCTCCGGTAATGATGAGTTTATTCGGCAATCTCATCGGCGACACGAACGC
CCACTTTCATGACCTGATTA

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTRPIQASVDLQALKQNLAIVRRAAPDARVWSVVKANAYGHGIERIWNALASTDGFALLNIEDAVTLRERGWKGPILMLE
GFFHADDLAVYDKYRLTTSVHSNWQLKALQNARLNAPLDIYVKVNSGMNRLGFPPEKIPTVWQQLRGMSNVGEMTLMSHF
ADAEHPEGIVQALEKVEQASEGMECRRSLANSAATLWHPQAHFDWVRPGIILYGASPTGHWCDIANTGLKPVMTLSSEII
GVQTLKAGDRVGYGGRYTASGEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTITVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV

Sequences:

>Translated_356_residues
MTRPIQASVDLQALKQNLAIVRRAAPDARVWSVVKANAYGHGIERIWNALASTDGFALLNIEDAVTLRERGWKGPILMLE
GFFHADDLAVYDKYRLTTSVHSNWQLKALQNARLNAPLDIYVKVNSGMNRLGFPPEKIPTVWQQLRGMSNVGEMTLMSHF
ADAEHPEGIVQALEKVEQASEGMECRRSLANSAATLWHPQAHFDWVRPGIILYGASPTGHWCDIANTGLKPVMTLSSEII
GVQTLKAGDRVGYGGRYTASGEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTITVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
>Mature_355_residues
TRPIQASVDLQALKQNLAIVRRAAPDARVWSVVKANAYGHGIERIWNALASTDGFALLNIEDAVTLRERGWKGPILMLEG
FFHADDLAVYDKYRLTTSVHSNWQLKALQNARLNAPLDIYVKVNSGMNRLGFPPEKIPTVWQQLRGMSNVGEMTLMSHFA
DAEHPEGIVQALEKVEQASEGMECRRSLANSAATLWHPQAHFDWVRPGIILYGASPTGHWCDIANTGLKPVMTLSSEIIG
VQTLKAGDRVGYGGRYTASGEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTITVGTVSMDMLAVDLTPCPQAGIGTPVE
LWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=356, Percent_Identity=86.7977528089888, Blast_Score=653, Evalue=0.0,
Organism=Escherichia coli, GI1790487, Length=346, Percent_Identity=42.7745664739884, Blast_Score=280, Evalue=9e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 38579; Mature: 38448

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPIQASVDLQALKQNLAIVRRAAPDARVWSVVKANAYGHGIERIWNALASTDGFALLN
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHEEECCCCCCHHHHHHHHHHCCCCEEEEE
IEDAVTLRERGWKGPILMLEGFFHADDLAVYDKYRLTTSVHSNWQLKALQNARLNAPLDI
EHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCEEE
YVKVNSGMNRLGFPPEKIPTVWQQLRGMSNVGEMTLMSHFADAEHPEGIVQALEKVEQAS
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EGMECRRSLANSAATLWHPQAHFDWVRPGIILYGASPTGHWCDIANTGLKPVMTLSSEII
HHHHHHHHHHCCHHEEECCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCHHHHHHHHHH
GVQTLKAGDRVGYGGRYTASGEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTITVGTVS
EEEEECCCCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCEEECCEEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
EEEEEEECCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEECC
>Mature Secondary Structure 
TRPIQASVDLQALKQNLAIVRRAAPDARVWSVVKANAYGHGIERIWNALASTDGFALLN
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHEEECCCCCCHHHHHHHHHHCCCCEEEEE
IEDAVTLRERGWKGPILMLEGFFHADDLAVYDKYRLTTSVHSNWQLKALQNARLNAPLDI
EHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCEEE
YVKVNSGMNRLGFPPEKIPTVWQQLRGMSNVGEMTLMSHFADAEHPEGIVQALEKVEQAS
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EGMECRRSLANSAATLWHPQAHFDWVRPGIILYGASPTGHWCDIANTGLKPVMTLSSEII
HHHHHHHHHHCCHHEEECCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCHHHHHHHHHH
GVQTLKAGDRVGYGGRYTASGEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTITVGTVS
EEEEECCCCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCEEECCEEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
EEEEEEECCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]