Click here to switch to the map view.

The map label for this gene is emtA

Identifier: 218549110

GI number: 218549110

Start: 1811565

End: 1812176

Strand: Reverse

Name: emtA

Synonym: EFER_1762

Alternate gene names: 218549110

Gene position: 1812176-1811565 (Counterclockwise)

Preceding gene: 218549113

Following gene: 218549108

Centisome position: 39.49

GC content: 50.16

Gene sequence:

>612_bases
GTGAAATTGAGATGGTTTGCCTTTTTAGTTGTGTTACTCGCTGGTTGTGCGACGAAGAGAGATTATGAAAATCCTCCATG
GAATGCCAAAGTCCCGGTACAGCGCGCAATGCAGTGGATGCCGATAAGCCAAAAGGCCGGAGCGGCCTGGAATGTCGATC
CGCAGTTAATTACTGCGATTATTGCCATTGAGTCTGGCGGTAATCCTAACGCAGTCAGTAAATCGAATGCTGTTGGCCTG
ATGCAACTCAAAGCCTCAACATCCGGTCGTGATGTTTATCGCCGTATGGGCTGGAGTGGTGAACCGACGACCAGTGAGCT
AAAAAACCCGGAGCGAAACATCTCCATGGGGGCAGCTTATTTGAACATTCTGGAAACCGGCCCCCTTGCCGGGATTAAAG
ATCCGCAGGTTTTACAATATGCGCTGGTGGTCTCCTATGCCAACGGTGCGGGGGCATTGCTACGGACCTTTTCATCTGAT
CGCAAAAAGGCGATCACTAAAATCAATGATCTGAGCGCAGATGAGTTTTTTGACCATGTGGCAAAAAATCATCCGGCACC
TCAGGCGCCGCGTTACATCTGGAAACTGCAACGCGCGCTGGATGAAATGTAG

Upstream 100 bases:

>100_bases
ATGGCAATTCCTTGCTGACAACTGAATGCAAATGTATATCATGCCGCGTAGGTATTGTGTTGTCACCTCAGAGACAAATT
CCGGCATAAGGATAGACGAA

Downstream 100 bases:

>100_bases
CGTCCTGGTACTTAGTTACGCACTTTACTGGCGCGTTCGCGCTCTTCCCGTTCAAGGGAAAATATAATCCGCTGTAATTC
CCGCTCCATCGCCGGGCCGA

Product: lytic murein endotransglycosylase E

Products: NA

Alternate protein names: Peptidoglycan lytic endotransglycosylase

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MKLRWFAFLVVLLAGCATKRDYENPPWNAKVPVQRAMQWMPISQKAGAAWNVDPQLITAIIAIESGGNPNAVSKSNAVGL
MQLKASTSGRDVYRRMGWSGEPTTSELKNPERNISMGAAYLNILETGPLAGIKDPQVLQYALVVSYANGAGALLRTFSSD
RKKAITKINDLSADEFFDHVAKNHPAPQAPRYIWKLQRALDEM

Sequences:

>Translated_203_residues
MKLRWFAFLVVLLAGCATKRDYENPPWNAKVPVQRAMQWMPISQKAGAAWNVDPQLITAIIAIESGGNPNAVSKSNAVGL
MQLKASTSGRDVYRRMGWSGEPTTSELKNPERNISMGAAYLNILETGPLAGIKDPQVLQYALVVSYANGAGALLRTFSSD
RKKAITKINDLSADEFFDHVAKNHPAPQAPRYIWKLQRALDEM
>Mature_203_residues
MKLRWFAFLVVLLAGCATKRDYENPPWNAKVPVQRAMQWMPISQKAGAAWNVDPQLITAIIAIESGGNPNAVSKSNAVGL
MQLKASTSGRDVYRRMGWSGEPTTSELKNPERNISMGAAYLNILETGPLAGIKDPQVLQYALVVSYANGAGALLRTFSSD
RKKAITKINDLSADEFFDHVAKNHPAPQAPRYIWKLQRALDEM

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Preferentially cleaves at a distance of more than two disaccharide units from the ends of the glycan chain

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI87081855, Length=203, Percent_Identity=91.1330049261084, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI87082191, Length=166, Percent_Identity=39.1566265060241, Blast_Score=133, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EMTA_ESCF3 (B7LSJ1)

Other databases:

- EMBL:   CU928158
- RefSeq:   YP_002382901.1
- EnsemblBacteria:   EBESCT00000123113
- GeneID:   7119935
- GenomeReviews:   CU928158_GR
- KEGG:   efe:EFER_1762
- GeneTree:   EBGT00050000011649
- HOGENOM:   HBG366204
- ProtClustDB:   PRK15470
- BioCyc:   EFER585054:EFER_1762-MONOMER
- HAMAP:   MF_01381
- InterPro:   IPR008258
- InterPro:   IPR000189

Pfam domain/function: PF01464 SLT

EC number: 3.2.1.- [C]

Molecular weight: Translated: 22386; Mature: 22386

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00922 TRANSGLYCOSYLASE; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRWFAFLVVLLAGCATKRDYENPPWNAKVPVQRAMQWMPISQKAGAAWNVDPQLITAI
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHH
IAIESGGNPNAVSKSNAVGLMQLKASTSGRDVYRRMGWSGEPTTSELKNPERNISMGAAY
HEEECCCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHH
LNILETGPLAGIKDPQVLQYALVVSYANGAGALLRTFSSDRKKAITKINDLSADEFFDHV
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AKNHPAPQAPRYIWKLQRALDEM
HHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLRWFAFLVVLLAGCATKRDYENPPWNAKVPVQRAMQWMPISQKAGAAWNVDPQLITAI
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHH
IAIESGGNPNAVSKSNAVGLMQLKASTSGRDVYRRMGWSGEPTTSELKNPERNISMGAAY
HEEECCCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHH
LNILETGPLAGIKDPQVLQYALVVSYANGAGALLRTFSSDRKKAITKINDLSADEFFDHV
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AKNHPAPQAPRYIWKLQRALDEM
HHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA