BacMap

Definition	Escherichia fergusonii ATCC 35469 chromosome, complete genome.
Accession	NC_011740
Length	4,588,711

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The map label for this gene is paaF [H]

Identifier: 218548955

GI number: 218548955

Start: 1647858

End: 1648625

Strand: Reverse

Name: paaF [H]

Synonym: EFER_1603

Alternate gene names: 218548955

Gene position: 1648625-1647858 (Counterclockwise)

Preceding gene: 218548956

Following gene: 218548954

Centisome position: 35.93

GC content: 54.3

Gene sequence:

>768_bases
ATGAACGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCTGCCGCACGTAATGCGCTGAA
TAATGCCCTGCTGACGCAACTGGTAAATGAACTGGACGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACTG
GTAATGCACGCTTTTTTGCCGCCGGGGCCGATCTCAACGAAATGGCAGAAAAAGATCTCGTGGCCACCTTAAACGATACA
CGCCCGCAGCTATGGGCGCGATTGCAGGCCTTTAACAAACCACTCATCGCAGCTGTCAACGGTTACGCGCTTGGTGCGGG
CTGCGAACTGGCATTGTTGTGTGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTTGGCA
TCATGCCTGGTGCAGGCGGAACGCAACGTTTAATTCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGGGCGGA
GAAAGCATCACTGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTTCCCAGCGATTTAACTCTCGAATATGC
CTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGG
AAGTGGCTCTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGC
ATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAA

Upstream 100 bases:

>100_bases
GTCTGGAACCGGATGAACTGGCCGCAGGCTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGAC
TTTGACGCTAAGGGGATGGC

Downstream 100 bases:

>100_bases
TGATGGAATTCATCCTCAGTCATATAGAAAAGGGCGTGATGACACTAACGCTCAACCGCCCGGAACGCCTGAACAGTTTT
AATGATGAGATGCACGCACA

Product: enoyl-CoA hydratase-isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNELIVSRQQRVLLLTLNRPAARNALNNALLTQLVNELDAAATDTSISVCVITGNARFFAAGADLNEMAEKDLVATLNDT
RPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLGG
ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
ISAFLQKRTPDFKGR

Sequences:

>Translated_255_residues
MNELIVSRQQRVLLLTLNRPAARNALNNALLTQLVNELDAAATDTSISVCVITGNARFFAAGADLNEMAEKDLVATLNDT
RPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLGG
ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
ISAFLQKRTPDFKGR
>Mature_255_residues
MNELIVSRQQRVLLLTLNRPAARNALNNALLTQLVNELDAAATDTSISVCVITGNARFFAAGADLNEMAEKDLVATLNDT
RPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLGG
ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
ISAFLQKRTPDFKGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=45.5284552845528, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=36.046511627907, Blast_Score=130, Evalue=8e-31,
Organism=Homo sapiens, GI31542718, Length=239, Percent_Identity=31.3807531380753, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI70995211, Length=232, Percent_Identity=32.7586206896552, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI150378541, Length=252, Percent_Identity=31.3492063492063, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI260274832, Length=263, Percent_Identity=26.2357414448669, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI45643119, Length=263, Percent_Identity=26.2357414448669, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI260275230, Length=263, Percent_Identity=26.2357414448669, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI20127408, Length=203, Percent_Identity=34.4827586206897, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI68989263, Length=252, Percent_Identity=30.952380952381, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI25453481, Length=259, Percent_Identity=26.6409266409266, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI51538573, Length=259, Percent_Identity=26.6409266409266, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI4757966, Length=253, Percent_Identity=28.8537549407115, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI51538581, Length=253, Percent_Identity=28.8537549407115, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI221136756, Length=264, Percent_Identity=28.030303030303, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI221136753, Length=264, Percent_Identity=28.030303030303, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4757968, Length=258, Percent_Identity=25.968992248062, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI49355787, Length=258, Percent_Identity=25.968992248062, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI221307494, Length=264, Percent_Identity=28.030303030303, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI116235487, Length=262, Percent_Identity=26.7175572519084, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI157694516, Length=223, Percent_Identity=28.2511210762332, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI213417737, Length=223, Percent_Identity=28.2511210762332, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI62530384, Length=229, Percent_Identity=26.6375545851528, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI261878539, Length=163, Percent_Identity=31.2883435582822, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157694520, Length=207, Percent_Identity=27.0531400966184, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=98.0392156862745, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=35.7692307692308, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=35.8778625954198, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788597, Length=245, Percent_Identity=32.2448979591837, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1788682, Length=153, Percent_Identity=40.5228758169935, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082183, Length=250, Percent_Identity=26, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=34.0782122905028, Blast_Score=95, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=47.2, Blast_Score=243, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=46.9135802469136, Blast_Score=221, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17540714, Length=235, Percent_Identity=31.4893617021277, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=31.1320754716981, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=217, Percent_Identity=29.9539170506912, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=208, Percent_Identity=32.6923076923077, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=29.1338582677165, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=233, Percent_Identity=29.6137339055794, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=262, Percent_Identity=28.2442748091603, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=222, Percent_Identity=25.6756756756757, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=147, Percent_Identity=36.734693877551, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=147, Percent_Identity=36.734693877551, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=149, Percent_Identity=36.241610738255, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=267, Percent_Identity=25.4681647940075, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=267, Percent_Identity=25.4681647940075, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=27.2251308900524, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=45.9677419354839, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=45.9677419354839, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=33.5849056603774, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI21357171, Length=253, Percent_Identity=29.6442687747036, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19920382, Length=263, Percent_Identity=30.0380228136882, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24654903, Length=212, Percent_Identity=28.7735849056604, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=24.7058823529412, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24650670, Length=257, Percent_Identity=26.4591439688716, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583165, Length=244, Percent_Identity=25.4098360655738, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921018, Length=235, Percent_Identity=26.3829787234043, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921000, Length=173, Percent_Identity=33.5260115606936, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583077, Length=173, Percent_Identity=33.5260115606936, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583079, Length=173, Percent_Identity=33.5260115606936, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27219; Mature: 27219

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELIVSRQQRVLLLTLNRPAARNALNNALLTQLVNELDAAATDTSISVCVITGNARFFA
CCCCHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
AGADLNEMAEKDLVATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAG
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCEEECCCCHHHHHHHHHHCC
ENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLGGESITAQQAQQAGLVSDVFPS
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCC
DLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHH
ISAFLQKRTPDFKGR
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MNELIVSRQQRVLLLTLNRPAARNALNNALLTQLVNELDAAATDTSISVCVITGNARFFA
CCCCHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
AGADLNEMAEKDLVATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAG
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCEEECCCCHHHHHHHHHHCC
ENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLGGESITAQQAQQAGLVSDVFPS
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCC
DLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHH
ISAFLQKRTPDFKGR
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]