BacMap

Definition	Escherichia fergusonii ATCC 35469 chromosome, complete genome.
Accession	NC_011740
Length	4,588,711

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The map label for this gene is paaG [H]

Identifier: 218548954

GI number: 218548954

Start: 1647070

End: 1647858

Strand: Reverse

Name: paaG [H]

Synonym: EFER_1602

Alternate gene names: 218548954

Gene position: 1647858-1647070 (Counterclockwise)

Preceding gene: 218548955

Following gene: 218548953

Centisome position: 35.91

GC content: 53.99

Gene sequence:

>789_bases
ATGATGGAATTCATCCTCAGTCATATAGAAAAGGGCGTGATGACACTAACGCTCAACCGCCCGGAACGCCTGAACAGTTT
TAATGATGAGATGCACGCACAACTGGCAGAGTGCCTGAAACAGGCGGAGCGCGACGACACTATCCGTTGCCTGTTAATTA
CAGGTGCCGGACGTGGATTTTGTGCTGGTCAGGATCTCAACGATCGTAACGTTGATCCCACTGGCCCCGCACCGGATTTA
GGTATGTCAGTGGAGCGATTCTATAACCCGCTGGTACGTCGCATGGCAAAACTGCCAAAACCAGTGATCTGTGCAGTCAA
TGGCGTGGCGGCAGGGGCTGGCGCAACACTGGCACTGGGGTGCGATATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCA
TGGCCTTTAGTAAATTAGGTTTAGTACCCGATTGCGGTGGTACCTGGTTACTGCCACGCGTTGCCGGGCGAGCGCGCGCC
ATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGATCTGGCAGGTTGTTGATGA
TGAAACGCTGGCAGATACCGCGCAACAGCAGGCACTGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGC
AAGCGATAAATAGCGCTGAAACTAACACGTTCGATACGCAGCTGGATCTGGAACGCGACTATCAGCGACTTGCCGGACGG
AGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAG

Upstream 100 bases:

>100_bases
AGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACG
CCCGACTTTAAAGGACGCTA

Downstream 100 bases:

>100_bases
CATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCAGAAGTTGCCGCCAGTC
ATGGACATCAGGTTTTACTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MMEFILSHIEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQAERDDTIRCLLITGAGRGFCAGQDLNDRNVDPTGPAPDL
GMSVERFYNPLVRRMAKLPKPVICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQQALHLATQPTFGLGLIKQAINSAETNTFDTQLDLERDYQRLAGR
SADYREGVSAFLAKRSPQFTGK

Sequences:

>Translated_262_residues
MMEFILSHIEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQAERDDTIRCLLITGAGRGFCAGQDLNDRNVDPTGPAPDL
GMSVERFYNPLVRRMAKLPKPVICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQQALHLATQPTFGLGLIKQAINSAETNTFDTQLDLERDYQRLAGR
SADYREGVSAFLAKRSPQFTGK
>Mature_262_residues
MMEFILSHIEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQAERDDTIRCLLITGAGRGFCAGQDLNDRNVDPTGPAPDL
GMSVERFYNPLVRRMAKLPKPVICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQQALHLATQPTFGLGLIKQAINSAETNTFDTQLDLERDYQRLAGR
SADYREGVSAFLAKRSPQFTGK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=30.6451612903226, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI45643119, Length=219, Percent_Identity=29.2237442922374, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI260275230, Length=219, Percent_Identity=29.2237442922374, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI260274832, Length=219, Percent_Identity=29.2237442922374, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=31.3432835820896, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI70995211, Length=204, Percent_Identity=30.8823529411765, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI157694516, Length=231, Percent_Identity=29.004329004329, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI213417737, Length=231, Percent_Identity=29.004329004329, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI20127408, Length=189, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI37594469, Length=181, Percent_Identity=31.4917127071823, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4757968, Length=208, Percent_Identity=27.8846153846154, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI49355787, Length=208, Percent_Identity=27.8846153846154, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI37594471, Length=181, Percent_Identity=31.4917127071823, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI221307494, Length=168, Percent_Identity=30.952380952381, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI221136756, Length=168, Percent_Identity=30.952380952381, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI221136753, Length=168, Percent_Identity=30.952380952381, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI25453481, Length=208, Percent_Identity=27.4038461538462, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI51538573, Length=208, Percent_Identity=27.4038461538462, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757966, Length=208, Percent_Identity=27.4038461538462, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI51538581, Length=208, Percent_Identity=27.4038461538462, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI68989263, Length=218, Percent_Identity=29.3577981651376, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI150378541, Length=262, Percent_Identity=28.2442748091603, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=27.0588235294118, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI116235487, Length=187, Percent_Identity=26.2032085561497, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI157694520, Length=206, Percent_Identity=27.6699029126214, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=96.5648854961832, Blast_Score=489, Evalue=1e-140,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=35.8778625954198, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=30.2681992337165, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=28.4046692607004, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=28.7937743190661, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=28.4210526315789, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=190, Percent_Identity=29.4736842105263, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=32.4, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=30.0395256916996, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17549921, Length=193, Percent_Identity=29.5336787564767, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=248, Percent_Identity=25.8064516129032, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=203, Percent_Identity=27.5862068965517, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=29.004329004329, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=256, Percent_Identity=28.90625, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=25, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=24.9027237354086, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25141363, Length=180, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=195, Percent_Identity=27.6923076923077, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=187, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=164, Percent_Identity=28.0487804878049, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=164, Percent_Identity=28.0487804878049, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=164, Percent_Identity=28.0487804878049, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=228, Percent_Identity=25.4385964912281, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=34.4827586206897, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=34.4827586206897, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=31.5175097276265, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=25.5725190839695, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357171, Length=223, Percent_Identity=29.5964125560538, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24654903, Length=201, Percent_Identity=25.8706467661692, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24650670, Length=212, Percent_Identity=26.8867924528302, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571730, Length=178, Percent_Identity=28.6516853932584, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571729, Length=178, Percent_Identity=28.6516853932584, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28447; Mature: 28447

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEFILSHIEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQAERDDTIRCLLITGAGRGF
CHHHHHHHHCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
CAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRMAKLPKPVICAVNGVAAGAGATLALG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEC
CDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARAMGLALLGNQLSAEQAHEWGM
CEEEEEECCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHCHHHHHHCCE
IWQVVDDETLADTAQQQALHLATQPTFGLGLIKQAINSAETNTFDTQLDLERDYQRLAGR
EEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
SADYREGVSAFLAKRSPQFTGK
CCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MMEFILSHIEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQAERDDTIRCLLITGAGRGF
CHHHHHHHHCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
CAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRMAKLPKPVICAVNGVAAGAGATLALG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEC
CDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARAMGLALLGNQLSAEQAHEWGM
CEEEEEECCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHCHHHHHHCCE
IWQVVDDETLADTAQQQALHLATQPTFGLGLIKQAINSAETNTFDTQLDLERDYQRLAGR
EEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
SADYREGVSAFLAKRSPQFTGK
CCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]