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The map label for this gene is pcaR [H]

Identifier: 218548835

GI number: 218548835

Start: 1515028

End: 1515831

Strand: Reverse

Name: pcaR [H]

Synonym: EFER_1478

Alternate gene names: 218548835

Gene position: 1515831-1515028 (Counterclockwise)

Preceding gene: 218548844

Following gene: 218548833

Centisome position: 33.03

GC content: 50.87

Gene sequence:

>804_bases
ATGGCGCATACAAAGAGGGAATCGATGAGTGATGTTATTGGGTACGCAAAAAATGATCCTAACTTTATGTTGTCGCTGGC
GCGAGGGCTTGAGGTACTCAATGCATTTACGCCACAGCGACAACGACTGACTATCTCGCAATTAAGTCAGAAAACACAGA
TTTCTCGCGCCTCTGTACGTCGCTGCCTCTACACTCTCGCTGCACTTGGCATGGTGCACAGCCCGGATGGTCGCCATTAC
GAATTACTGCCCCGCGTACTGGCGGTTGGTCATGCATATCTTGCCGGAACGCCATTAGCAAAGGTGGCTCAGACGGCGCT
TGATAGCCTGGGTAAGAGCCTTGGACAATCCTGTTCTGCTGCAACTCTTGATGGTGATAACGTGCTCTATATTGCCCGCT
CAGCAGTCAATAATTTGCTTAGTGTAGATATTGGTCGTGGTAGTCGATTACCCGCATGGGCGACCTCTATGGGGAGAGTT
CTGCTGAGTGCACTACCTGATGAACAATTAAACGTGACGTTGTCACGTATAAACATGATTCGCTATACCCCGCATACCCT
GAATTCACTTTCTGCTTTACGTGAAGAACTGGCGCGAGTGCGCAGACAAGGCTATGCCCTGGCAGATCGGCAAATCGAGG
TCGGTCTGTGTTCTCTGGCGGTCCCGTTATTATCCCGAAATAACCAGGTGGTTGCCGCGTTGAACGTGGGTGTCCCGTCT
GCGTCTATGAGTGGCGCGGAACTGAAAGAACTAGCACTTCCCGCTTTGCGCCGTGTCGCTATGGATTTATCCTTACAGTT
GTAA

Upstream 100 bases:

>100_bases
ATCGGCACAAATGTTAACTTGAGGTTAATTGTGTGATCGAGGTGGTATAATGCAGTACTGCTGATGCCACATTTTTGGCA
ATGGGTATAGTAAATTTCGC

Downstream 100 bases:

>100_bases
TCATTACTGTTTAATTCAATATGGGGAATTATCCCTTCGGGCAAACGAAAATTGCCTGATTAACTACGCTAATCAGGCCT
TTAGTCTCTCTGTAATACAC

Product: transcriptional regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MAHTKRESMSDVIGYAKNDPNFMLSLARGLEVLNAFTPQRQRLTISQLSQKTQISRASVRRCLYTLAALGMVHSPDGRHY
ELLPRVLAVGHAYLAGTPLAKVAQTALDSLGKSLGQSCSAATLDGDNVLYIARSAVNNLLSVDIGRGSRLPAWATSMGRV
LLSALPDEQLNVTLSRINMIRYTPHTLNSLSALREELARVRRQGYALADRQIEVGLCSLAVPLLSRNNQVVAALNVGVPS
ASMSGAELKELALPALRRVAMDLSLQL

Sequences:

>Translated_267_residues
MAHTKRESMSDVIGYAKNDPNFMLSLARGLEVLNAFTPQRQRLTISQLSQKTQISRASVRRCLYTLAALGMVHSPDGRHY
ELLPRVLAVGHAYLAGTPLAKVAQTALDSLGKSLGQSCSAATLDGDNVLYIARSAVNNLLSVDIGRGSRLPAWATSMGRV
LLSALPDEQLNVTLSRINMIRYTPHTLNSLSALREELARVRRQGYALADRQIEVGLCSLAVPLLSRNNQVVAALNVGVPS
ASMSGAELKELALPALRRVAMDLSLQL
>Mature_266_residues
AHTKRESMSDVIGYAKNDPNFMLSLARGLEVLNAFTPQRQRLTISQLSQKTQISRASVRRCLYTLAALGMVHSPDGRHYE
LLPRVLAVGHAYLAGTPLAKVAQTALDSLGKSLGQSCSAATLDGDNVLYIARSAVNNLLSVDIGRGSRLPAWATSMGRVL
LSALPDEQLNVTLSRINMIRYTPHTLNSLSALREELARVRRQGYALADRQIEVGLCSLAVPLLSRNNQVVAALNVGVPSA
SMSGAELKELALPALRRVAMDLSLQL

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=226, Percent_Identity=31.4159292035398, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788131, Length=234, Percent_Identity=29.0598290598291, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI87082359, Length=231, Percent_Identity=25.1082251082251, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1789999, Length=255, Percent_Identity=24.3137254901961, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786715, Length=222, Percent_Identity=24.7747747747748, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28867; Mature: 28736

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHTKRESMSDVIGYAKNDPNFMLSLARGLEVLNAFTPQRQRLTISQLSQKTQISRASVR
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RCLYTLAALGMVHSPDGRHYELLPRVLAVGHAYLAGTPLAKVAQTALDSLGKSLGQSCSA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
ATLDGDNVLYIARSAVNNLLSVDIGRGSRLPAWATSMGRVLLSALPDEQLNVTLSRINMI
EEECCCCEEEEEHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCHHHEEEHHHHEEE
RYTPHTLNSLSALREELARVRRQGYALADRQIEVGLCSLAVPLLSRNNQVVAALNVGVPS
EECCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCC
ASMSGAELKELALPALRRVAMDLSLQL
CCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AHTKRESMSDVIGYAKNDPNFMLSLARGLEVLNAFTPQRQRLTISQLSQKTQISRASVR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RCLYTLAALGMVHSPDGRHYELLPRVLAVGHAYLAGTPLAKVAQTALDSLGKSLGQSCSA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
ATLDGDNVLYIARSAVNNLLSVDIGRGSRLPAWATSMGRVLLSALPDEQLNVTLSRINMI
EEECCCCEEEEEHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCHHHEEEHHHHEEE
RYTPHTLNSLSALREELARVRRQGYALADRQIEVGLCSLAVPLLSRNNQVVAALNVGVPS
EECCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCC
ASMSGAELKELALPALRRVAMDLSLQL
CCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]