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The map label for this gene is ansP [H]

Identifier: 218548817

GI number: 218548817

Start: 1498316

End: 1499815

Strand: Reverse

Name: ansP [H]

Synonym: EFER_1456

Alternate gene names: 218548817

Gene position: 1499815-1498316 (Counterclockwise)

Preceding gene: 218548827

Following gene: 218548815

Centisome position: 32.68

GC content: 52.07

Gene sequence:

>1500_bases
ATGAGATCACATAACAACGACGTAACTGATCAACACGCCGCGAAACGCCGCTGGCTTAATGCCCACGAAGAGGGGTATCA
CAAAGCGATGGGCAATCGGCAGGTACAGATGATCGCCATCGGCGGCGCGATTGGCACCGGCTTGTTTTTAGGTGCAGGAG
CCCGACTGCAAATGGCGGGTCCTGCGCTGGCACTGGTTTATTTAATATGTGGCTTGTTTTCGTTTTTTATTCTGCGGGCA
TTGGGTGAGCTGGTGTTACATCGCCCTTCCAGTGGCAGCTTTGTTTCTTATGCCCGAGAGTTTTTAGGAGAGAAAGCCGC
TTACGTTGCAGGCTGGATGTACTTCATTAACTGGGCGATGACCGGGATTGTCGATATCACCGCCGTCGCACTGTATATGC
ATTACTGGGGCGCATTTGGTGGTGTACCGCAGTGGGTATTTGCGCTCGCTGCGCTAACCATCGTTGGCACCATGAATATG
ATTGGTGTGAAATGGTTTGCGGAGATGGAGTTCTGGTTTGCGCTTATTAAAGTGCTCGCCATCGTGACCTTTTTGGTCGT
GGGTACAGTGTTCCTCGGTAGCGGTCAGCCGCTGGATGGCAACACCACTGGCTTTCATTTAATTACCGATAATGGCGGCT
TCTTCCCCCACGGTTTACTGCCTGCGCTGGTGTTGATTCAGGGCGTAGTGTTTGCTTTTGCCTCTATTGAAATGGTTGGA
ACAGCTGCCGGAGAATGTAAAGATCCGCAGACCATGGTGCCTAAAGCGATTAACAGCGTGATTTGGCGTATTGGCCTGTT
TTACGTCGGCTCCGTGGTGTTGCTGGTTATGTTATTGCCGTGGAGCGCGTATCAGGCTGGGCAAAGTCCGTTTGTGACGT
TCTTTTCCAAACTGGGCGTGCCGTACATCGGCAGCATTATGAACATTGTGGTGCTGACCGCTGCGCTCTCCAGCCTGAAC
TCAGGTCTGTACTGCACCGGACGTATTCTGCGCTCAATGGCGATGGGCGGTTCCGCACCGAGTTTTATGGCGAAAATGAG
CCGTCAGCATGTGCCGTATGCCGGGATCCTGGCGACGCTGGTCGTTTATGTTGTTGGCGTATTCCTTAACTATCTGGTGC
CGTCTCGCGTATTTGAGATTGTCCTGAACTTCGCGTCGCTGGGAATCATCGCTTCATGGGCGTTTATCATCGTGTGCCAG
ATGCGCCTGCGTAAAGCAATTAAAGAAGGCAAAGCAGCGGATGTCAGTTTTAAACTGCCTGGCGCGCCCTTCACTTCCTG
GCTGACATTACTGTTTTTACTGAGTGTCCTGGTGCTGATGGCGTTCGATTACCCGAACGGGACTTACACCATCGCGGCGC
TGCCGATTATCGGTATTCTGCTGGTTATAGGCTGGTTTGGTGTGCGCAAACGCGTTGCTGAAATTCACAGCACTGCGCCA
GTCGTCGAGGAAGATGAAGAAAAACAGGAAATTGTGTTTAAGCCTGAAACGGCGAGCTAA

Upstream 100 bases:

>100_bases
CCATAAAGAATAACGGTTAGCGCTGTCATAGTTACCGTGAATAAATAATGTATTTATGGTAACGAGAAACGTTAATTCAT
GAAGATCATCAGGGAGCGCA

Downstream 100 bases:

>100_bases
TTGCAAAATTGCCCGGCTACGTGCCGGGCAAATTTAGATTACTGACGAAGGTGTGCCGCATGCGGCGTGATAAAAGTCAG
GCAAGTCTCTCCGTTTTCAC

Product: L-asparagine transporter

Products: Proton [Cytoplasm]; L-asparagine [Cytoplasm] [C]

Alternate protein names: L-asparagine transport protein [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MRSHNNDVTDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAGPALALVYLICGLFSFFILRA
LGELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVG
TAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLN
SGLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQ
MRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGILLVIGWFGVRKRVAEIHSTAP
VVEEDEEKQEIVFKPETAS

Sequences:

>Translated_499_residues
MRSHNNDVTDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAGPALALVYLICGLFSFFILRA
LGELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVG
TAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLN
SGLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQ
MRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGILLVIGWFGVRKRVAEIHSTAP
VVEEDEEKQEIVFKPETAS
>Mature_499_residues
MRSHNNDVTDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAGPALALVYLICGLFSFFILRA
LGELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVG
TAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLN
SGLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQ
MRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGILLVIGWFGVRKRVAEIHSTAP
VVEEDEEKQEIVFKPETAS

Specific function: Unknown

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Homo sapiens, GI4507047, Length=430, Percent_Identity=23.0232558139535, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI258645169, Length=415, Percent_Identity=21.6867469879518, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI87081915, Length=499, Percent_Identity=98.7975951903808, Blast_Score=997, Evalue=0.0,
Organism=Escherichia coli, GI1786302, Length=456, Percent_Identity=36.8421052631579, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1786789, Length=420, Percent_Identity=37.6190476190476, Blast_Score=307, Evalue=8e-85,
Organism=Escherichia coli, GI1790653, Length=459, Percent_Identity=37.2549019607843, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1786602, Length=453, Percent_Identity=36.4238410596026, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI48994972, Length=421, Percent_Identity=38.7173396674584, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI87081708, Length=438, Percent_Identity=33.7899543378995, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1789017, Length=423, Percent_Identity=33.5697399527187, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1788480, Length=470, Percent_Identity=32.3404255319149, Blast_Score=228, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI17533459, Length=436, Percent_Identity=22.4770642201835, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17532491, Length=437, Percent_Identity=21.7391304347826, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324061, Length=465, Percent_Identity=29.0322580645161, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6320772, Length=438, Percent_Identity=29.4520547945205, Blast_Score=190, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6322892, Length=431, Percent_Identity=33.1786542923434, Blast_Score=186, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6324990, Length=502, Percent_Identity=26.6932270916335, Blast_Score=180, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6324924, Length=513, Percent_Identity=28.4600389863548, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321629, Length=424, Percent_Identity=29.0094339622642, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324059, Length=467, Percent_Identity=28.6937901498929, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321053, Length=461, Percent_Identity=27.3318872017354, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324553, Length=399, Percent_Identity=30.5764411027569, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6322967, Length=534, Percent_Identity=26.7790262172285, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6320717, Length=416, Percent_Identity=28.125, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324981, Length=423, Percent_Identity=28.3687943262411, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320251, Length=458, Percent_Identity=26.6375545851528, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319824, Length=434, Percent_Identity=26.2672811059908, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6319543, Length=424, Percent_Identity=27.3584905660377, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6319608, Length=458, Percent_Identity=25.5458515283843, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319542, Length=410, Percent_Identity=26.0975609756098, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320364, Length=503, Percent_Identity=21.0735586481113, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI221512776, Length=424, Percent_Identity=21.2264150943396, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24666159, Length=424, Percent_Identity=21.2264150943396, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24668806, Length=441, Percent_Identity=22.4489795918367, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356285, Length=441, Percent_Identity=22.4489795918367, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24668802, Length=441, Percent_Identity=22.4489795918367, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 54286; Mature: 54286

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHNNDVTDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAG
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCEEECC
PALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
TGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNMIGVKWFAEMEFWFALIKVLA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVG
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHCCC
PYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHH
VVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQMRLRKAIKEGKAADVSFKLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
GAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGILLVIGWFGVRKRVAEIHSTAP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVEEDEEKQEIVFKPETAS
CCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MRSHNNDVTDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAG
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCEEECC
PALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
TGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNMIGVKWFAEMEFWFALIKVLA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVG
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHCCC
PYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHH
VVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQMRLRKAIKEGKAADVSFKLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
GAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGILLVIGWFGVRKRVAEIHSTAP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVEEDEEKQEIVFKPETAS
CCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-asparagine [Periplasm] + Proton [Periplasm] = Proton [Cytoplasm] + L-asparagine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]