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The map label for this gene is fumA [H]

Identifier: 218548786

GI number: 218548786

Start: 1464197

End: 1465843

Strand: Direct

Name: fumA [H]

Synonym: EFER_1425

Alternate gene names: 218548786

Gene position: 1464197-1465843 (Clockwise)

Preceding gene: 218548780

Following gene: 218548787

Centisome position: 31.91

GC content: 49.18

Gene sequence:

>1647_bases
ATGTCAAATAAACCCTTCCATTATCAGGCTCCTTTTCCACTCAAGAAAGATGATACCGAGTACTATCTGCTGTCCAGCGA
ACATGTCACTGTCTCTGAATTTGAAGGACAGGAGATCTTAAAAGTTGCTCCCGAAGCACTGACTCTGTTGGCGAGACAGG
CGTTTCATGATGCCTCATTTATGCTGCGTCCTGCCCATCAACAGCAGGTAGCTGATATTTTGCGTGACCCTGAAGCCAGT
GAAAACGATAAATATGTCGCACTGCAATTCCTGCGCAACTCGGATATCGCGGCAAAAGGCGTATTGCCAACCTGCCAGGA
TACCGGTACAGCCATTATCGTTGGCAAAAAAGGTCAGCGTGTATGGACTGGTGGAGGTGATGAAGCTGCGCTGGCACGTG
GTGTGTACGATACCTACATTGAAGATAACCTGCGCTATTCACAAAACGCCGCGCTTGATATGTACAAAGAAGTGAACACC
GGTACTAACCTGCCAGCCCAGATCGATCTCTACGCGGTTGATGGTGATGAGTATAAATTCCTTTGCATCGCGAAAGGTGG
TGGTTCGGCAAACAAAACGTATCTCTATCAGGAAACCAAAGCATTACTGACACCGGGGAAACTGAAAAATTACCTGGTTG
AGAAAATGCGCACACTGGGTACAGCAGCTTGCCCGCCTTATCATATTGCGTTTGTTATCGGTGGCACTTCTGCTGAAACC
AACCTTAAAACAGTGAAACTGGCGTCGGCGAAATACTATGACGAGCTACCAACGGAAGGGAATGAGCATGGTCAGGCATT
CCGTGATGTTGAGCTGGAAAAAGAGTTGCTGATCGAAGCACAGAATCTGGGGCTGGGAGCGCAGTTCGGCGGTAAATATT
TCGCCCATGATATCCGTGTCATCCGTCTGCCACGCCATGGAGCATCTTGCCCGGTTGGTATGGGCGTCTCATGTTCTGCT
GACCGTAATATTAAAGCGAAGATCAATCGCCAGGGTATCTGGATTGAGAAGCTGGAACATAACCCAGGGAAATACATTCC
TGAAGAGCTGCGTAAAGCGGGAGAAGGTGAGGCCGTACGTGTTGATTTAAATCGTCCGATGAGTGAAATCCTCGCTCAGT
TGTCGCAGTATCCGGTATCAACGCGCTTATCGCTGAACGGCACCATTATTGTCGGTCGTGATATTGCACACGCTAAACTG
AAAGAGCGTATGGATAACGGGGAAGGTCTGCCACAGTACATTAAAGATCACCCAATTTATTATGCAGGTCCGGCGAAAAC
ACCGGCAGGATACGCATCAGGATCTCTTGGCCCAACTACCGCCGGACGTATGGACTCTTATGTCGATCAGTTACAGTCCC
ACGGTGGCAGCATGATCATGCTGGCCAAAGGTAACCGTAGCCAGCAGTTAACAGATGCCTGTAAAAAACACGGTGGCTTC
TACCTCGGCAGTATTGGCGGCCCGGCAGCGGTCTTGGCCCAGGGCAGCATTAAGAGCCTTGAGTGTGTTGAGTACCCGGA
GCTTGGCATGGAAGCTATCTGGAAAATTGAAGTCGAAGATTTCCCGGCATTTATCCTTGTGGATGACAAAGGAAATGACT
TCTTCCAACAGATACAAATGTCGCAGTGTTCGCGCTGCGTCAAGTAA

Upstream 100 bases:

>100_bases
GTTTTTGTTAAATATTTGTGTATGATGCTGTTACCTGGTTTTTAACGAGTGTCGCAAGATACTGAGAGAAAAAACTGAAC
GAGCAGTAAGTGAGAGAACA

Downstream 100 bases:

>100_bases
TCGCCAGGTAAGCCGTCCTTCGGGGCGGCTTTTTATTGTTATTAAATAGTAAATACTTTGTTGGGTATGTGCTCTAAGTA
ATTTGAGTCGCAGCACATGT

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSNKPFHYQAPFPLKKDDTEYYLLSSEHVTVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEAS
ENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYDTYIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET
NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMSEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKL
KERMDNGEGLPQYIKDHPIYYAGPAKTPAGYASGSLGPTTAGRMDSYVDQLQSHGGSMIMLAKGNRSQQLTDACKKHGGF
YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQMSQCSRCVK

Sequences:

>Translated_548_residues
MSNKPFHYQAPFPLKKDDTEYYLLSSEHVTVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEAS
ENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYDTYIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET
NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMSEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKL
KERMDNGEGLPQYIKDHPIYYAGPAKTPAGYASGSLGPTTAGRMDSYVDQLQSHGGSMIMLAKGNRSQQLTDACKKHGGF
YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQMSQCSRCVK
>Mature_547_residues
SNKPFHYQAPFPLKKDDTEYYLLSSEHVTVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEASE
NDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYDTYIEDNLRYSQNAALDMYKEVNTG
TNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETN
LKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSAD
RNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMSEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLK
ERMDNGEGLPQYIKDHPIYYAGPAKTPAGYASGSLGPTTAGRMDSYVDQLQSHGGSMIMLAKGNRSQQLTDACKKHGGFY
LGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQMSQCSRCVK

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=97.8102189781022, Blast_Score=1119, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=89.7810218978102, Blast_Score=1031, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=168, Percent_Identity=27.9761904761905, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60204; Mature: 60073

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPFHYQAPFPLKKDDTEYYLLSSEHVTVSEFEGQEILKVAPEALTLLARQAFHDASF
CCCCCEEEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHH
MLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALARGVYDTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAVDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRV
CEEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEHHCCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVR
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCCEEE
VDLNRPMSEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIY
EECCCCHHHHHHHHHHCCCEEEEEECEEEEEECCHHHHHHHHHHCCCCCCHHHHHCCCEE
YAGPAKTPAGYASGSLGPTTAGRMDSYVDQLQSHGGSMIMLAKGNRSQQLTDACKKHGGF
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQM
EEECCCCCEEEEECCCCCCEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
SQCSRCVK
HHHHHHCC
>Mature Secondary Structure 
SNKPFHYQAPFPLKKDDTEYYLLSSEHVTVSEFEGQEILKVAPEALTLLARQAFHDASF
CCCCEEEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHH
MLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALARGVYDTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAVDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRV
CEEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEHHCCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVR
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCCEEE
VDLNRPMSEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIY
EECCCCHHHHHHHHHHCCCEEEEEECEEEEEECCHHHHHHHHHHCCCCCCHHHHHCCCEE
YAGPAKTPAGYASGSLGPTTAGRMDSYVDQLQSHGGSMIMLAKGNRSQQLTDACKKHGGF
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQM
EEECCCCCEEEEECCCCCCEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
SQCSRCVK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]