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The map label for this gene is manA [H]

Identifier: 218548785

GI number: 218548785

Start: 1462826

End: 1464061

Strand: Reverse

Name: manA [H]

Synonym: EFER_1424

Alternate gene names: 218548785

Gene position: 1464061-1462826 (Counterclockwise)

Preceding gene: 218548788

Following gene: 218548784

Centisome position: 31.91

GC content: 48.87

Gene sequence:

>1236_bases
TTGCCAGAGAATTCTTGCTTTAATAACGCCAACAAATTACACATGAAACAGGGCTTGATAATGCAAAAACTCATTAACTC
AGTGCAGAACTATGCCTGGGGAAGTAAAACAGCGTTAACGGATCTTTACGGTATCGCCAACCCATCCCAACAACCGATGG
CCGAGTTATGGATGGGCGCACACCCGAAAAGCAGCTCAAAAGTTGAAACATCTGAAGGACAAATCGTCTCACTGCGTGAT
GTTATTGAGAACGATAAATCAGCCCTGCTTGGGGATGCAGTCGCGGAGCGTTTTGGCGAACTCCCGTTTTTGTTCAAAGT
GTTGTGTGCCGCGCAACCGCTCTCTATTCAGGTGCACCCTAACAAACGTAACTCTGAAATCGGCTTTGCCAAAGAAAATG
CAGCAGGTATTCCAATGGATGCCGCCGAGCGAAATTATAAAGATCCCAATCATAAACCAGAGTTAGTTTTTGCCCTGACA
CCGTTCCTTGCAATGAACGCGTTCCGTGAATTCTCTGAGATTGTTTCGTTGCTGCAACCTGTTGCGGGGGCGCATCCGGC
AATTGCCCATTTTTTACAGCAACCTGATGCCGAACGTTTAAGTGAACTGTTTGCCGCCCTGCTCAATATGCAGGGAGAGG
AAAAATCCCGCGCGCTGGCCATTCTGAAATCGGCACTCGACACTCAGCAGGGTGAACCGTGGCAGACGATTCGCGTGATC
GCGCAGTTTTATCCAGATGACAGCGGCCTGTTCTCACCACTGCTGCTCAATGTGGTGAAACTGAATCCTGGTGAAGCCAT
GTTCCTGTTTGCCGAAACGCCACACGCTTATTTGCAGGGCGTGGCTCTGGAAGTCATGGCCAACTCCGATAACGTATTAC
GCGCCGGTTTGACACCAAAATATATTGATATTCCTGAGCTGGTTGCCAACGTTAAGTTTGAAGCGAAACCCGCGGGACAA
TTGTTGACGCAGCCCATCAAAAACGGTCATGAACTTGACTTCCCTATTCCGGTTGAGGATTTTGCGTTTGCTTTGCATGA
CCTGACGAAAGACGAAGCTGCCGTTAACCAGCAAAGTGCGGCAATCCTGTTCTGCGTTGAAGGTGAAGCCTGTTTGCGCA
AAGGTGATCAACAGTTACATCTGAAGCCTGGCGAATCTGCTTTTATAGCCGCAAACGAGTCGCCGGTTTTAGTGAGTGGC
AATGGTCGTTTAGCCCGCGTTTACAACAAGCTGTAA

Upstream 100 bases:

>100_bases
TCAGTATCTTGCGACACTCGTTAAAAACCAGGTAACAGCATCATACACAAATATTTAACAAAAACTGAGACTAGTACGAC
TTTTTAAGCCCACAGGTTAC

Downstream 100 bases:

>100_bases
TGGCATACTGAAATATTTAACAACTCTTGCTAAGCTTATTAAAGGCTTATAACACCACCAGGCGGTATGTTCCGCCTGGT
TTCATTTTACGGATAAAAAA

Product: mannose-6-phosphate isomerase

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MPENSCFNNANKLHMKQGLIMQKLINSVQNYAWGSKTALTDLYGIANPSQQPMAELWMGAHPKSSSKVETSEGQIVSLRD
VIENDKSALLGDAVAERFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALT
PFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERLSELFAALLNMQGEEKSRALAILKSALDTQQGEPWQTIRVI
AQFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPAGQ
LLTQPIKNGHELDFPIPVEDFAFALHDLTKDEAAVNQQSAAILFCVEGEACLRKGDQQLHLKPGESAFIAANESPVLVSG
NGRLARVYNKL

Sequences:

>Translated_411_residues
MPENSCFNNANKLHMKQGLIMQKLINSVQNYAWGSKTALTDLYGIANPSQQPMAELWMGAHPKSSSKVETSEGQIVSLRD
VIENDKSALLGDAVAERFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALT
PFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERLSELFAALLNMQGEEKSRALAILKSALDTQQGEPWQTIRVI
AQFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPAGQ
LLTQPIKNGHELDFPIPVEDFAFALHDLTKDEAAVNQQSAAILFCVEGEACLRKGDQQLHLKPGESAFIAANESPVLVSG
NGRLARVYNKL
>Mature_410_residues
PENSCFNNANKLHMKQGLIMQKLINSVQNYAWGSKTALTDLYGIANPSQQPMAELWMGAHPKSSSKVETSEGQIVSLRDV
IENDKSALLGDAVAERFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTP
FLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERLSELFAALLNMQGEEKSRALAILKSALDTQQGEPWQTIRVIA
QFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPAGQL
LTQPIKNGHELDFPIPVEDFAFALHDLTKDEAAVNQQSAAILFCVEGEACLRKGDQQLHLKPGESAFIAANESPVLVSGN
GRLARVYNKL

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide [H]

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family [H]

Homologues:

Organism=Homo sapiens, GI4505235, Length=406, Percent_Identity=34.2364532019704, Blast_Score=186, Evalue=5e-47,
Organism=Escherichia coli, GI1787899, Length=391, Percent_Identity=89.2583120204604, Blast_Score=723, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71997620, Length=404, Percent_Identity=30.9405940594059, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17557650, Length=418, Percent_Identity=28.2296650717703, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320839, Length=414, Percent_Identity=33.3333333333333, Blast_Score=183, Evalue=3e-47,
Organism=Drosophila melanogaster, GI21356061, Length=401, Percent_Identity=32.4189526184539, Blast_Score=161, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01238 PMI_typeI [H]

EC number: =5.3.1.8 [H]

Molecular weight: Translated: 45021; Mature: 44890

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00965 PMI_I_1 ; PS00966 PMI_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPENSCFNNANKLHMKQGLIMQKLINSVQNYAWGSKTALTDLYGIANPSQQPMAELWMGA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHCCC
HPKSSSKVETSEGQIVSLRDVIENDKSALLGDAVAERFGELPFLFKVLCAAQPLSIQVHP
CCCCCCCEECCCCCEEEHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECC
NKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
VAGAHPAIAHFLQQPDAERLSELFAALLNMQGEEKSRALAILKSALDTQQGEPWQTIRVI
HCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
AQFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPK
HHHCCCCCCCHHHHHHHHHEECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEEECCCCC
YIDIPELVANVKFEAKPAGQLLTQPIKNGHELDFPIPVEDFAFALHDLTKDEAAVNQQSA
CCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCC
AILFCVEGEACLRKGDQQLHLKPGESAFIAANESPVLVSGNGRLARVYNKL
EEEEEECCHHHHHCCCCEEEECCCCCEEEEECCCCEEEECCCCEEHHHHCC
>Mature Secondary Structure 
PENSCFNNANKLHMKQGLIMQKLINSVQNYAWGSKTALTDLYGIANPSQQPMAELWMGA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHCCC
HPKSSSKVETSEGQIVSLRDVIENDKSALLGDAVAERFGELPFLFKVLCAAQPLSIQVHP
CCCCCCCEECCCCCEEEHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECC
NKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
VAGAHPAIAHFLQQPDAERLSELFAALLNMQGEEKSRALAILKSALDTQQGEPWQTIRVI
HCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
AQFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPK
HHHCCCCCCCHHHHHHHHHEECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEEECCCCC
YIDIPELVANVKFEAKPAGQLLTQPIKNGHELDFPIPVEDFAFALHDLTKDEAAVNQQSA
CCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCC
AILFCVEGEACLRKGDQQLHLKPGESAFIAANESPVLVSGNGRLARVYNKL
EEEEEECCHHHHHCCCCEEEECCCCCEEEEECCCCEEEECCCCEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6397402; 9097039; 9278503 [H]